Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2011
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Timetable
Tue 13:00–14:50 C13/209
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2010
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Timetable
Tue 13:00–14:50 C13/209
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2009
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Timetable
Tue 13:00–14:50 C04/218
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered (the classification is dependent on the report quality and the correctness of results)
Language of instruction
English
Further Comments
The course is taught annually.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2023

The course is not taught in Autumn 2023

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2022

The course is not taught in Autumn 2022

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
autumn 2021

The course is not taught in autumn 2021

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2020

The course is not taught in Autumn 2020

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2019

The course is not taught in Autumn 2019

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2018

The course is not taught in Autumn 2018

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
autumn 2017

The course is not taught in autumn 2017

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2016

The course is not taught in Autumn 2016

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2015

The course is not taught in Autumn 2015

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2014

The course is not taught in Autumn 2014

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2013

The course is not taught in Autumn 2013

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2012

The course is not taught in Autumn 2012

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 26 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2011 - acreditation

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Literature
    recommended literature
  • Creighton, T.E., ed. Protein function: A practical approach. 2nd ed. 1997, Oxford University Press: Oxford. 334.
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2010 - only for the accreditation, Autumn 2009, Autumn 2010, Autumn 2011.

Bi8858c Protein Preparation and Characterization II - practice

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
0/2. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: graded credit. Other types of completion: z (credit).
Teacher(s)
prof. RNDr. Zbyněk Prokop, Ph.D. (seminar tutor)
doc. Mgr. Radka Chaloupková, Ph.D. (seminar tutor)
Guaranteed by
prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Zbyněk Prokop, Ph.D.
Prerequisites
NOW( Bi8858 Biocatalysis-Enzyme Technogy )
attending the theoretical course Bi8858 "Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology" is prerequisite for attending this practical course Bi8858c
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 10 student(s).
Current registration and enrolment status: enrolled: 0/10, only registered: 0/10, only registered with preference (fields directly associated with the programme): 0/10
fields of study / plans the course is directly associated with
there are 20 fields of study the course is directly associated with, display
Course objectives
The practical course will focus on methodology usefull for structural and kinetic characterisation of proteins/enzymes. The practical exercises will teach students to characterize secondary structure and thermostability of proteins by method of circular dichroism, quarternary structure will be probed by using of native electrophoresis and gel chromatography. The students will learn how to assess basic kinetic parametrs of enzymatic reactions by using colorimetric methods and gas chromatography.
Syllabus
  • 1. Protein folding and stability
  • 2. Quarternary structure of proteins
  • 3. Enzyme activity and kinetics
Teaching methods
laboratory practicals, protocols prepration, homeworks
Assessment methods
the course is finnished when all laboratory practicals are conducted and all corresponding reports are well prepared and delivered
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.
  • Enrolment Statistics (recent)