C4182 Biochemistry II

Faculty of Science
Spring 2025
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 17. 2. to Sat 24. 5. Tue 10:00–11:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024.

C4182 Biochemistry II

Faculty of Science
Spring 2024
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 19. 2. to Sun 26. 5. Tue 10:00–11:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2023
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 1. 3. to Fri 14. 5. Tue 10:00–11:50 online_BCH1
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2020
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 10:00–11:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2019
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 18. 2. to Fri 17. 5. Mon 14:00–15:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
spring 2018
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 14:00–15:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Learning outcomes
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology. They will learn metabolism of nitrogen containing compounds, genetic code, principles of replication, transcription and tranlation.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2017
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 20. 2. to Mon 22. 5. Mon 14:00–15:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2016
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 14:00–15:50 B11/132
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2015
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 14:00–15:50 B11/132
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2014
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 12:00–13:50 B11/132
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Syllabus
  • Biochemistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslational modifications. Point mutations, insertion, deletion. Regulation of genes expression in prokaryotes (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosemonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetic balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decarboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffusion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation). Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones). Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2013
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 8:00–9:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
This course is an extension of the Biochemistry I. It carries next important knowledges about biochemical reactions in living cell. It describes the metabolism of proteins and nucleic acids, proteosynthesis and its regulation, basic metods of study and practical applications. It describes also other functions of proteins, carbohydrates and lipids, some special metabolic pathways and indicates the connections between chemical reactions and physiological features in an organism.
Students will acquire important knowledges here, necessary for full understanding of basic principles of biochemical basis of life, and required for next special courses in biochemistry and molecular biology.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2012
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 9:00–10:50 B11/205
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 11:00–12:50 B11/132
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2010
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Tue 9:00–10:50 aula_Vinařská
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2009
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Fri 10:00–11:50 A,01026
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2008
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 14:00–15:50 A,01026
Prerequisites
C3190 Biochemistry I - sem.
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemie II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolismus nukleových kyselin a proteosyntéza. Syntéza a odbourání bazí. Degradace a syntéza NK. Fosfodiesterasy, palindrom, restrikční endonukleasy. Replikace DNA, replikační vidlička, DNA polymerasa. Transkripce DNA a její faktory, mRNA, kodon, souvislost mezi strukturou bílkoviny a kodony, reverzní transkripce, translace, struktura tRNA, antikodon, ribosomy, translace, posttranslační modifikace. Mutace bodové, inzeční a deleční, Regulace exprese genů u prokaryontů (inducibilní, represibilní systém, operon, represor, regulátorový gen) 4. Metabolismus sacharidů, štěpení a syntéza polysacharidů (škrob, glykogen). Interkonverse monosacharidů. Přímá oxidace glukosy, význam. Pentosový cyklus. Anaerobní glykolýza, její jednotlivé kroky, energetická bilance. Substrátová fosforylace. Glukoneogeneze, syntéza PEP. Coriho cyklus.. Oxidační dekarboxylace pyruvátu. 5. Metabolismus lipidů, odbourání a syntéza tuků a fosfolipidů. Odbourání a syntéza mastných kyselin. Ketonické látky. 6. Citrátový cyklus, reakce, význam, energetická bilance. Anabolický význam, anaplerotické reakce, glyoxylátový cyklus. 7. Redoxní reakce v biochemii. Respirační řetězec, jeho komponenty (cytochromy, ubichinon), struktura komplexů 1-4. Oxidační fosforylace, chemiosmotická teorie, protonmotivní síla a transmembránový potenciál. Syntéza ATP, struktura ATPsyntasy. Inhibitory respirace a syntéza ATP, rozpojovače, ionofory. Bilance oxidační fosforylace. Alternativní respirace. Mikrosomální elektronový transport, cyt P450. Nitrogenasový systém. 8. Fotosyntéza, světelná fáze, chlorofyly, struktura fotosyntetického centra, Komponenty přenosu elektronů (cytochromy, chinony, plastocyanin, ferredoxin), FS-2, FS-1, mechanismus syntézy ATP. Rovnice světelné fáze a její bilance. Temná fáze fotosyntézy (Calvinův cyklus), RUBISCO, mechanismus fixace CO2. 9. Membránový transport, usnadněná difuze, aktivní transport, permeasy a iontové kanály. Transport aminokyselin, cukrů a iontů. Symport, antiport. Fúze membrán. Struktura a funkce K-Na-ATPasy, laktosový přenašeč, struktura K-kanálu. Přenos nervového vzruchu. 10. Principy metabolických regulací, úrovně, mechanismy (regulace konečným produktem, energetický náboj. Allosterie, kooperativita, Hillova rovnice). Molekulové základy hormonální regulace, hierarchie a struktura. 11. Isoprenoidy, karotenoidy, steroidy, (cholesterol, jeho syntéza, konformace, žlučové kyseliny, vitamin D, steroidní hormony). Syntéza porfyrinů - hemu, odbourání hemu, regulace. 12. Základní procesy syntézy aromátů (šikimátová a polyketidová cesta). Sekundární metabolity, obecné reakce syntézy alkaloidů.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2007
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Timetable
Mon 9:00–10:50 02004
Prerequisites
C3190 Biochemistry I - sem.
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2006
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Timetable
Mon 9:00–10:50 02004
Prerequisites
C3190 Biochemistry I - sem.
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2005
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Timetable
Tue 9:00–10:50 02004
Prerequisites
C3190 Biochemistry I - sem.
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2004
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2002
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites (in Czech)
C3181 Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2001
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites (in Czech)
C3181 Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2000
Extent and Intensity
2/2/0. 5 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites (in Czech)
C3181 Biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 18 fields of study the course is directly associated with, display
Syllabus
  • Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Language of instruction
Czech
Further Comments
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
spring 2012 - acreditation

The information about the term spring 2012 - acreditation is not made public

Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2011 - only for the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Petr Zbořil, CSc. (lecturer)
Guaranteed by
doc. RNDr. Petr Zbořil, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Prerequisites
C3181 Biochemistry I
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
At the end of the course students should be able to understand and explain - Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • Biochemiistry II 1. Catabolism of proteins and amino acids. Peptidases, classification, importance, specificity.Degradation of amino acids, transamination, biogenic amines. Special catabolic pathways (viz. aromatic and essential.) Genetic diseases in amino acid metabolism. 2. Nitrogen excretion, the role of glutamate dehydrogenase, glutamine synthetase, ornithine cycle. Uric acid. Ammonia assimilation. 3. Metabolism of nucleic acids and proteosynthesis. Synthesis and degradation of bases. Breakdown and synthesis of nucleic acids. Phosphodiesterases, palindromes, restriction endonucleases. DNA replication, replication fork, DNA polymerase. Transcription of DNA and its factors, mRNA, codon, relationship between protein structure and codons, reverse transcription, tRNA structure, anticodon, ribosomes, translation, posttranslation modifications. Point mutations, insertion, deletion. Regulation of genes expression in procaryonts (inducible and repressive systems, operon, repressor). 4. Carbohydrate metabolism, degradation and synthesis of polysaccharides (starch, glycogen). Interconversion of monosaccharides. Hexosomonophosphate pathway, importance. Pentose cycle. Glycolysis, individual steps, energetical balance. Substrate-level phosphorylation. Gluconeogenesis, PEP synthesis. Cori cycle. Oxidative decyrboxylation of pyruvate. 5. The metabolism of lipids. Degradation and synthesis of fats and phospholipids. Degradation and synthesis of fatty acids. Ketone bodies. 6. Tricarboxylic acids cycle, reactions, importance, energetic balance. Anabolic aspects, anaplerotic reactions, glyoxylate shunt. 7. Redox reactions in biochemistry. Respiratory chain, its components (cytochrome, ubiquinone), structure of complexes I - IV. Oxidative phosphorylation, chemiosmotic coupling, protonmotive force, transmembrane potential. ATP synthesis, structure of ATP synthase. Inhibitors of respiration and ATP synthesis, uncouplers, ionophores. Energetic balance. Alternative respirations. Microsomal electron transport, cyt P450. Nitrogenase system. 8. Photosynthesis, light reactions, chlorophylls, structure of reaction centre. Components of electron transfer (cytochromes, quinones, plastocyanine, ferredoxin), PS-1, PS-2, mechanism of ATP synthesis. Equation and balance of dark phase. Dark reactions (Calvin cycle), RUBISCO, mechanism of CO2 fixation. 9. Membrane transport, facilitated diffussion, active transport, permeases, ion channels. Transport of ions, sugars and amino acids. Symport and antiport. Membrane fusion. Structure and function of Na-K-ATPase, lactose transporter, K-channel structure. Neurotransmission. 10. Principles of metabolic regulations, levels, mechanisms (endproduct regulation - feedback inhibition, energetic potential, allosteric effects, cooperativity, Hill equation.) Molecular backgrounds of humoral regulation, structure and hierarchy of hormone system. 11. Isoprenoids, carotenoids, steroids (cholesterol, its synthesis, conformation, bile acids, vitamins D, steroid hormones.) Porphyrins, heme synthesis and degradation, regulation. 12. Basic reaction and pathways in synthesis of aromates (shikimate and polyketide pathways.) Secondary metabolites, general reactions in synthesis of alkaloids.
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • ŠÍPAL, Zdeněk. Biochemie. 1. vyd. Praha: Státní pedagogické nakladatelství, 1992, 479 s. ISBN 8004217362. info
  • Mikes, V. - Základní pojmy z biochemie
Teaching methods
Lectures.
Assessment methods
Basic lecture. Written examination.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.

C4182 Biochemistry II

Faculty of Science
Spring 2008 - for the purpose of the accreditation
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Vladimír Mikeš, CSc. (lecturer)
Guaranteed by
prof. RNDr. Vladimír Mikeš, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: prof. RNDr. Vladimír Mikeš, CSc.
Prerequisites
C3190 Biochemistry I - sem.
Basic knowledge of biochemistry I
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 17 fields of study the course is directly associated with, display
Course objectives
Basic catabolic and anabolic pathways. Glycolysis, citrate cycle, respiration. Synthesis and degradation of aminoacids, fatty acids, proteins, cabohydrates. Photosynthesis. Regulation of meabolic pathways. Physiological processes and their biochemical nature.
Syllabus
  • 1. Methods of isolation of biopolymers, synthesis of biopolymers 2. Methods of characterization of proteins, synthesis of nucleotides, DNA, RNA 3. Inhibitions of enzymes activity, two-substrate reactions, regulations of enzymes activity (pH, temperature, allostery, cooperativity, 4. Covalent modification, feed back inhibiton 5. Biochemistry of hemoglobin, pathology of hemoglobin 6. DNA replication, DNA polymerase, transcription, translation, mRNA, codon, anticodon, ribosomes, synthesis of proteins in procaryotes, regulation of gene expression 7. Krebs cycle, oxidatuve decarboxylation, pentosaphosphate pathway 8. Degradation and synthesis of lipids, oxidation of fatty acids, synthesis of fatty acids, acetogenesis 9. Metabolism of aminoacids 10. Urea cycle, nitrogen metabolism 11. Redox reaction, respiration chain, oxidative phosphorylation, chemiosmotic theory, ATP stnthesis 12. Membrane transport 13. Photosynthesis, chlorophyll, dark phase, RUBISCO 14. Physiological biochemistry
Literature
  • Biochemie. Edited by Zdeněk Vodrážka. 2. opr. vyd. Praha: Academia, 1996, 191 s. ISBN 80-200-0600-1. info
  • Biochemie. Edited by Daniel Voet - Judith G. Voet, Translated by A. Maelicke - W. Müller-E. Weinheim: VCH Verlagsgesellschaft, 1992, 1237 s. ISBN 3-527-28242-4. info
  • Mikes, V. - Základní pojmy z biochemie
Assessment methods (in Czech)
Základní přednáška doplněná seminářem, navazuje základní biochemické praktikum.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Listed among pre-requisites of other courses

Zobrazit další předměty

The course is also listed under the following terms Spring 2011 - only for the accreditation, Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (recent)