Course Information

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
In-person direct teaching

Teacher(s)

Mgr. Gabriel Demo, Ph.D. (lecturer)
RNDr. Petr Těšina, Ph.D. (lecturer)
prof. RNDr. Michaela Wimmerová, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Michaela Wimmerová, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Tue 15:00–16:50 C04/211

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course introduces students to the structure of proteins and to the dynamic behaviour of proteins & their combinations in relation to the biological functions of proteins.

Learning outcomes

The lecture summarises the basic knowledge about the structure and function of proteins. The first part of the lecture discusses the structural motifs of proteins, their stability and conformational dynamics. In the second part, selected biological functions of proteins (e.g., enzymes, membrane proteins, protein-ligand interactions) are discussed. In the third part, basic molecular biology techniques for protein isolation, gene cloning and expression, and structural biology for determining the 3D structures of proteins are presented.

Syllabus

• 1. Basic structural principles of protein architecture. Building blocks of proteins. Protein structure motifs. Post-translational modifications of proteins. Stability and dynamics of proteins.
• 2. Enzymes and enzyme catalysis. Proteins interacting with ligands (e.g., DNA and RNA molecules, transcription and translation factors). Membrane proteins, unstructured proteins.
• 3. Methods of preparation of recombinant DNA molecules. Isolation and cloning of genes. DNA sequencing. Production of recombinant proteins. Prediction and modelling of protein structure. Biophysical methods for the determination of the 3D structure of proteins (protein crystallography, cryo-electron microscopy, NMR, SAXS).

Literature

recommended literature
• KESSEL, Amit a BEN-TAL Nir. Introduction to proteins. Structure, function and motion. 2nd ed. CRC Press, Taylor and Francis, Boca Raton, Florida USA, 2018, 880 s. ISBN 978-1-4987-4717-2
• PETSKO Gregory & RINGE Dagmar. Protein structure and function. Oxford University Press, 2008, 224 stran. ISBN 9780199556847.
• WHITFORD, David. Proteins : structure and function. Chichester: John Wiley & Sons, 2005, xiv, 528. ISBN 0471498947. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi8202 Proteomics - a basic course
• CB070 Protein crystallography
• C6215 Advanced biochemistry and its methods
• C7270 Structural biology methods
• C9940 3-Dimensional Transmission Electron Microscopy (3DEM)

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

Mgr. Gabriel Demo, Ph.D. (lecturer)

Guaranteed by

Mgr. Gabriel Demo, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Tue 15:00–16:50 C04/211

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course introduces students to the structure of proteins and to the dynamic behaviour of proteins & their combinations in relation to the biological functions of proteins.

Learning outcomes

The lecture summarises the basic knowledge about the structure and function of proteins. The first part of the lecture discusses the structural motifs of proteins, their stability and conformational dynamics. In the second part, selected biological functions of proteins (e.g., enzymes, membrane proteins, protein-ligand interactions) are discussed. In the third part, basic molecular biology techniques for protein isolation, gene cloning and expression, and structural biology for determining the 3D structures of proteins are presented.

Syllabus

• 1. Basic structural principles of protein architecture. Building blocks of proteins. Protein structure motifs. Post-translational modifications of proteins. Stability and dynamics of proteins.
• 2. Enzymes and enzyme catalysis. Proteins interacting with ligands (e.g., DNA and RNA molecules, transcription and translation factors). Membrane proteins, unstructured proteins.
• 3. Methods of preparation of recombinant DNA molecules. Isolation and cloning of genes. DNA sequencing. Production of recombinant proteins. Prediction and modelling of protein structure. Biophysical methods for the determination of the 3D structure of proteins (protein crystallography, cryo-electron microscopy, NMR, SAXS).

Literature

recommended literature
• TORRES, Anton. Protein Science: Structure and Mechanism. States Academic Press, New York USA, 2022, 249 s. ISBN 1639894497
• KESSEL, Amit a BEN-TAL Nir. Introduction to proteins. Structure, function and motion. 2nd ed. CRC Press, Taylor and Francis, Boca Raton, Florida USA, 2018, 880 s. ISBN 978-1-4987-4717-2

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course introduces students to the structure of proteins and to the dynamic behaviour of proteins & their combinations in relation to the biological functions of proteins.

Learning outcomes

The lecture summarises the basic knowledge about the structure and function of proteins. The first part of the lecture discusses the structural motifs of proteins, their stability and conformational dynamics. In the second part, selected biological functions of proteins (e.g., enzymes, membrane proteins, protein-ligand interactions) are discussed. In the third part, basic molecular biology techniques for protein isolation, gene cloning and expression, and structural biology for determining the 3D structures of proteins are presented.

Syllabus

• 1. Basic structural principles of protein architecture. Building blocks of proteins. Protein structure motifs. Post-translational modifications of proteins. Stability and dynamics of proteins.
• 2. Enzymes and enzyme catalysis. Proteins interacting with ligands (e.g., DNA and RNA molecules, transcription and translation factors). Membrane proteins, unstructured proteins.
• 3. Methods of preparation of recombinant DNA molecules. Isolation and cloning of genes. DNA sequencing. Production of recombinant proteins. Prediction and modelling of protein structure. Biophysical methods for the determination of the 3D structure of proteins (protein crystallography, cryo-electron microscopy, NMR, SAXS).

Literature

recommended literature
• KESSEL, Amit a BEN-TAL Nir. Introduction to proteins. Structure, function and motion. 2nd ed. CRC Press, Taylor and Francis, Boca Raton, Florida USA, 2018, 880 s. ISBN 978-1-4987-4717-2

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 17. 9. to Fri 14. 12. Tue 17:00–18:50 C03

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 18. 9. to Fri 15. 12. Tue 15:00–16:50 C04/211

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course makes students familiar with structural motifs recurring in protein structures and their relation to biological functions of proteins, and their engineering.

Learning outcomes

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 19. 9. to Sun 18. 12. Mon 17:00–18:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 17:00–18:50 B11/306

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
Mgr. Tomáš Klumpler, Ph.D. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Wed 18:00–19:50 B11/306

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 17:00–18:50 B11/132

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 15:00–16:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Thu 16:00–17:50 B11/205

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 11:00–12:50 C04/211

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

zk (examination). Alternate Types of Completion: k (colloquium).**

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Fri 10:00–11:50 C04/211

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

zk (examination). Alternate Types of Completion: k (colloquium).**

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Tue 10:00–11:50 BFU

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Assessment methods

zk (examination). Alternate Types of Completion: k (colloquium).**

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

Study Materials
The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Mon 16:00–17:50 BFU

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Timetable

Tue 8:00–9:50 BFU

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Timetable

Fri 9:00–10:50 Kontaktujte učitele

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
RNDr. Alena Kuderová, CSc. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
RNDr. Alena Kuderová, CSc. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Proteinové inženýrství

Extent and Intensity

2/0/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
RNDr. Alena Kuderová, CSc. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

Předpokladem pro porozumění předmětu je absolvování základů biochemie nebo molekulární biologie.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives (in Czech)

Přednáška je uvedena shrnutím strukturních motivů polypeptidových řetězců a principů vytváření strukturních domén. Následně se zaměřuje se na roli jednotlivých motivů v biologické funkci proteinů. Budou diskutovány strukturní prvky podmiňující specificitu interakcí transkripčních faktorů a polymeráz s DNA.Bude probrána molekulární podmíněnost plasticity odpovědi imunitního systému na expozici novým antigenum. V oblasti membránových proteinů bude položen důraz na charakteristiky transmembranových domén, využití jejich strukturních specifik k předpovedi jejich výskytu na základě primárni sekvence aminokyselin u nově objevených proteinů, detailněji budou osvětleny strukturně-funkční vztahy u bakteriorhodopsinu,fotosyntetického reakčního centra, membranových receptorů, jejich interakce s G proteiny a strukturní poruchy jejichž manifestací jsou některá nadorová onemocnění. Bude diskutována enzymová katalýza a možnosti modifikace substrátové specificity a reakční rychlosti. Součástí přednášky budou také metody předpovídání sekundární a terciární struktury proteinů na základě jejich aminokyselinové sekvence, počítačové modelování struktur proteinů, jejich interakce s ligandy a modelování enzymatických reakcí a rovněž metody stanovení trojrozměrné struktury proteinů. Na závěr bude shrnuto využití technik genového inženýrství pro studium vztahu struktury a funkce proteinů. Na zajištění přednášky participují RNDr. Jiři Damborský,CSc. (Počítačové modelování v proteinovém inženýrství) a RNDr. Alena Kuderová, CSc. (Rozpoznávání cizorodých molekul imunitním systémem).

Syllabus (in Czech)

• 1.Základní strukturní principy architektury proteinů. 2.Role jednotlivých strukturních motivů v biologické funkci proteinů. -Proteiny interagující s DNA -Rozpoznávání cizorodých molekul imunitním systémem -Membránové proteiny (membránové receptory) -Enzymová katalýza -Předpovídání, modelování a navrhování cíleného obměňování struktury proteinů -Metody stanovení trojrozměrné struktury proteinů 3.Použití technik genového inženýrství pro studium vztahu struktury a funkce proteinů -Metody přípravy rekombinantních molekul DNA, izolace klonovaných genů, genetické elementy řídící expresi genů -Mutageneze in vitro -Produkce rekombinantních proteinů v heterologních expresních systémech

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• B8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Proteinové inženýrství

Extent and Intensity

2/0/0. 3 credit(s). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
RNDr. Alena Kuderová, CSc. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Prerequisites (in Czech)

Předpokladem pro porozumění předmětu je absolvování základů biochemie nebo molekulární biologie.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives (in Czech)

Přednáška je uvedena shrnutím strukturních motivů polypeptidových řetězců a principů vytváření strukturních domén. Následně se zaměřuje se na roli jednotlivých motivů v biologické funkci proteinů. Budou diskutovány strukturní prvky podmiňující specificitu interakcí transkripčních faktorů a polymeráz s DNA.Bude probrána molekulární podmíněnost plasticity odpovědi imunitního systému na expozici novým antigenum. V oblasti membránových proteinů bude položen důraz na charakteristiky transmembranových domén, využití jejich strukturních specifik k předpovedi jejich výskytu na základě primárni sekvence aminokyselin u nově objevených proteinů, detailněji budou osvětleny strukturně-funkční vztahy u bakteriorhodopsinu,fotosyntetického reakčního centra, membranových receptorů, jejich interakce s G proteiny a strukturní poruchy jejichž manifestací jsou některá nadorová onemocnění. Bude diskutována enzymová katalýza a možnosti modifikace substrátové specificity a reakční rychlosti. Součástí přednášky budou také metody předpovídání sekundární a terciární struktury proteinů na základě jejich aminokyselinové sekvence, počítačové modelování struktur proteinů, jejich interakce s ligandy a modelování enzymatických reakcí a rovněž metody stanovení trojrozměrné struktury proteinů. Na závěr bude shrnuto využití technik genového inženýrství pro studium vztahu struktury a funkce proteinů. Na zajištění přednášky participují RNDr. Jiři Damborský,CSc. (Počítačové modelování v proteinovém inženýrství) a RNDr. Alena Kuderová, CSc. (Rozpoznávání cizorodých molekul imunitním systémem).

Syllabus (in Czech)

• 1.Základní strukturní principy architektury proteinů. 2.Role jednotlivých strukturních motivů v biologické funkci proteinů. -Proteiny interagující s DNA -Rozpoznávání cizorodých molekul imunitním systémem -Membránové proteiny (membránové receptory) -Enzymová katalýza -Předpovídání, modelování a navrhování cíleného obměňování struktury proteinů -Metody stanovení trojrozměrné struktury proteinů 3.Použití technik genového inženýrství pro studium vztahu struktury a funkce proteinů -Metody přípravy rekombinantních molekul DNA, izolace klonovaných genů, genetické elementy řídící expresi genů -Mutageneze in vitro -Produkce rekombinantních proteinů v heterologních expresních systémech

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• B8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Proteinové inženýrství

Extent and Intensity

2/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
Chemistry Section – Faculty of Science

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Biochemistry (programme PřF, M-CH)
• Biochemistry (programme PřF, N-CH)

Language of instruction

Czech

Further Comments

The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

C7920 Structure and functions of proteins

The information about the term Autumn 2011 - acreditation is not made public

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

Oral exam or colloquium.

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

At the end of the course students should know principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Teaching methods

lectures

Assessment methods

zk (examination). Alternate Types of Completion: k (colloquium).**

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

C7920 Structure and functions of proteins

Extent and Intensity

2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).

Teacher(s)

prof. RNDr. Břetislav Brzobohatý, CSc. (lecturer)
prof. Mgr. Jiří Damborský, Dr. (lecturer)
doc. RNDr. Jaromír Marek, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Břetislav Brzobohatý, CSc.
National Centre for Biomolecular Research – Faculty of Science

Prerequisites

Completion of basic courses of biochemistry and/or molecular biology is a prerequisite for subscription to the course.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

there are 9 fields of study the course is directly associated with, display

Course objectives

The course summarizes principles of protein structure and function. In the first part, individual structural motifs recurring in protein structures are described, and ways are shown how proteins with quite unrelated functions are built up from combinations of these. In the second part, particular biological functions are considered and the different protein structural solutions are discussed that have evolved to fulfill them. In the third part, the basic techniques of protein structural biology are introduced and examples of engineering protein structure and function are shown.

Syllabus

• 1. Basic structural principles of protein architecture. The building blocks of proteins. Motifs of protein structure. Domain structure of proteins. 2. The roles of individual motifs of protein structure in protein biological functions. DNA interacting proteins, transcription factors, receptors. Molecular recognition of foreign molecules by immune system. Membrane proteins, membrane receptors. Enzyme catalysis. Prediction, engineering, and design of protein structures. Determination of protein structure. 3. Genetic engineering techniques for structure-function analysis in proteins. Recombinant DNA construction. Gene isolation and cloning. Gene expression regulating elements. DNA sequencing. In vitro mutagenesis. Recombinant protein production in heterologous expression systems.

Literature

• BRANDEN, Carl and John TOOZE. Introduction to protein structure. 2nd ed. New York: Garland Publishing, 1998, xiv, 410 s. ISBN 0-8153-2304-2. info

Language of instruction

Czech

Follow-Up Courses

• Bi7201 Genomics - a basic course
• Bi8202 Proteomics - a basic course

Further Comments

The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.

Listed among pre-requisites of other courses

• C6210 Biotechnology
  (Bi1700 || C7920 || CG010 || C1470 || souhlas) && !NOWANY(C6211)  

Teacher's information

http://ncbr.chemi.muni.cz/~jiri/courses.html

• Enrolment Statistics (recent)