Bi8090 Gene engineering

Faculty of Science
Spring 2011
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
prof. RNDr. Jiří Doškař, CSc. (lecturer)
Guaranteed by
prof. RNDr. Jiří Doškař, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: prof. RNDr. Jiří Doškař, CSc.
Timetable
Thu 8:00–9:50 B11/306
Prerequisites
Ex_2979 Metody molekulární biologie || Ex_2980 Metody molekulární genetiky || B6390 Molecular Methods || B6400 Methods of molecular biology || Bi6400 Methods of molecular biology || NOW( Bi6400 Methods of molecular biology ) || SOUHLAS
Going through the basic lecture from Molecular biology and Methods of Molecular Biology; it is recommended to go through the lectures from Molecular Biology of Prokaryotes, Molecular Biology of Viruses, Molecular biology of Eukaryotes
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course students should be able to understand the basic principles of gene engineering, preparation of transgenic organisms and their use in the research and in industry, medicine and agriculture.
Syllabus
  • Definition of gene engineering, its history and perspectives
  • Mutagenesis in vitro
  • Protein engineering
  • Optimalization of gene expression
  • Gene cloning in bacteria
  • Gene cloning in plants
  • gene cloning in animals
  • Characteristics of cloning vectors
  • Gene transfer in embryonal cells
  • Gene therapy
  • Preparation of pharmacologically important substances in heterologous systems
  • Risc of manipulations with GMO
  • Ethical problems
Literature
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
  • PRIMROSE, S. B. and Richard M. TWYMAN. Principles of gene manipulation and genomics. 7th ed. Malden, Mass.: Blackwell Publishing, 2006, xxii, 644. ISBN 1405135441. info
Teaching methods
Lectures are based on power-point pictures, tables and texts elaborated according to textbooks, monographs and scientific papers wich are explained and complemented with live commentaries. All the presentations are available on IS.
Assessment methods
Oral examination follows the written test in which students should answer 8-10 questions covering main topics of the lecture. During the oral examinations the students have to prove their knowledge on particular examples. To pass the exam they should answer 70% of the answers.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2011, recent)
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