C7072 Bioanalytics II - Analytical methods in clinical praxis

Faculty of Science
Spring 2022
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
doc. Mgr. Jan Havliš, Dr. (lecturer)
Guaranteed by
doc. Mgr. Jan Havliš, Dr.
National Centre for Biomolecular Research – Faculty of Science
Supplier department: National Centre for Biomolecular Research – Faculty of Science
Timetable
Thu 8:00–9:50 C02/211
Prerequisites
basic knowledge of biochemistry, of analytical, organic and inorganic chemistry are prerequisited.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
main objective of the course is to introduce the bioanalytics to its main field - laboratory medicine and diagnostics - to the students.
Learning outcomes
at the end of the course students should be able:
to define relation between bioanalysis and illness diagnosis
to evaluate and apply respective bioanalytical approaches starting with method selection, through analyte determination to results presentation
to understand principles of basic methodical approaches and common instrumental techniques.
Syllabus
  • 1. – introduction, samples – state specific analyte, sample types and their properties, preanalytical phase
  • 2. – classic instrumentation – analysers with liquid and solid reagencies; organisation, integration and miniaturisation of analyses; analytical sets
  • 3. – clinical metobolomics – metabolite, metabolome, metabolomics/metabonomics; basic concepts, importance, goals and demands; analytical platforms, data
  • 4. – advanced instrumentation – chromatography, electrophoresis, mass spectrometry
  • 5. – immunoanalysis – antigen, antibody; methods of Ag-Ab complex detection, immunodiagnostics, immunohistochemistry, immunocytochemistry
  • 6. – analysis of proteins – non-specific and specific, protein chips, enzyme analysis, enzyme as analyte and enzyme as analytical agent; role of buffers in bioanalysis
  • 7. – colourness – physical phenomenon and its utilisation in bioanalysis; medical microbiology, cultivation, biochemical and proteomic evidence, sensitivity to antibiotics
  • 8. – analysis of DNA – genetics and epigenetics in clinical praxis; NA preparation approaches, cytogenetics, identification of known and unknown sequence, methylation analysis
  • 9. – quality in clinical analysis – check and control; calibration and reference materials, operative quality control, validation, GLP
  • 10. – analytical methods – choice and optimisation; analytical method characteristics, choice of analytical methods
  • 11. – analytic result – its presentation; interpretation, uncertainty sources, variability; case study – development of a method for clinical diagnostics
  • 12. – selected analytes – inorganic, organic and macromolecular bioanalytes
Literature
  • CHROMÝ, Vratislav. Bioanalytika : analytická chemie v laboratorní medicíně. 1. vyd. Brno: Masarykova univerzita v Brně, 2002, 267 s. ISBN 80-210-2917-X. info
  • CHROMÝ, Vratislav and Jiří FISCHER. Analytické metody v klinické chemii. 1. vyd. Brno: Masarykova univerzita v Brně, 2000, 215 s. ISBN 8021023635. info
Teaching methods
the lecture is based on ppt presentation and its explication. presentation itself will be available as a study material (black-and-white printable pdf with high resolution and restricted access rights). it is recommended to attend the lecture, because of the explication, which significantly extends the presentation and because there are no available textbooks in Czech language covering certain parts of the subject.
Assessment methods
oral examination; students are required to understand and to be familiar with the principles and their applications. examination consists of three basic questions, which would be during the examination expanded to let the student demonstrate the extent of topic understanding.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
The course is also listed under the following terms Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2022/C7072