Bi5491 Molecular Diagnostics - Practice I

Faculty of Science
Autumn 2007 - for the purpose of the accreditation
Extent and Intensity
0/4/0. 4 credit(s) (plus extra credits for completion). Type of Completion: z (credit).
Teacher(s)
učitelé GMB (seminar tutor)
doc. RNDr. Petr Kuglík, CSc. (seminar tutor)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. Mgr. Petr Beneš, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Petr Kuglík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Petr Kuglík, CSc.
Prerequisites
(SOUHLAS || ( B4070 Obecná mikrobiologie || B4090 General microbiology || B4090p General microbiology -l || Bi4090c General microbiology - pr. || BX090 || BMB22 General microbiology || B2050 General microbiology || Bi2050c General microbiology pr. )) && ( Bi4035 Molecular biology - lab.pract. || Bi4020c Molecular biology - practice )
Molecular structure a function of procaryotic and eucaryotic genome. Cultiovation of bacteria, estimation of titre, CFU.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 0/15, only registered: 0/15
fields of study / plans the course is directly associated with
Course objectives
The subject is a part of basic course Molecular biology for students of discipline Molecular and cellular diagnostic. It comprises the tasks that offer acquiring of basic methods in molecular biology of bacteria and their viruses.
Syllabus
  • 1. Makrorestrikční analýza genomové DNA pomocí pulzní-gelové elektroforézy. 2. Neradioaktivní značení sond digoxigeninem. 3. Southernův přenos a selektivní hybridizace bakteriální DNA se specifickými sondami. 4. PCR detekce specifických genů u bakterií. 5. Izolace proteinu z zivocisnych bunek, elektroforeza SDS-PAGE, westernovy prenos, protilatkova detekce specifickeho proteinu. 6. Stanovení počtu živočišných buněk v populaci s použitím hemocytometru a mikroskopie. 7.-12. Specializované úlohy z molekulární diagnostiky prováděné na externích odborných pracovištích.
Literature
  • Návody k úlohám jsou dostupné u vyučujících
  • PCR Protocols for Emerging Infectious Diseases :a supplement to Diagnostic molecular microbiology : principles and applications. Edited by David H. Persing. Washington: ASM Press, 1996, xii, 180 s. ISBN 1-55581-108-6. info
  • PERSING, D.H., T.F. SMITH, F.C. TENOVER and T.J. WHITE. Diagnostic molecular microbiology. Washington, D.C.: ASM Press, 1993, 642 pp. ISBN 1-55581-056-X. info
  • MILLER, Jeffrey H. A Short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press, 1992, 456 s. ISBN 0879693495. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009.