Bi8858 Protein Preparation and Characterization II - Biocatalysis and Enzyme Technology

Přírodovědecká fakulta
podzim 2009
Rozsah
2/0. 2 kr. (příf plus uk plus > 4). Ukončení: zk.
Vyučující
prof. RNDr. Zbyněk Prokop, Ph.D. (přednášející)
Garance
prof. Mgr. Jiří Damborský, Dr.
Ústav experimentální biologie – Biologická sekce – Přírodovědecká fakulta
Kontaktní osoba: prof. RNDr. Zbyněk Prokop, Ph.D.
Rozvrh
Út 11:00–12:50 C04/211
Předpoklady
! C8858 Biocatalysis
- basic course of biochemistry recommended - course of microbiology and molecular biology helpful
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Cíle předmětu
Enzyme technology – a subfield of biotechnology – offers modern solutions for the development of new products, processes and services to meet various human needs. The goal of this inovative approach utilising enzymes as biocatalysts is not only to design competetive processes but also meet criteria of sustainability (reduction of raw material, energy and water consumption, reduction of waste production and overall environmental impact together with increasing usage of renewable materials such as biomass). The aim of the course is to provide insight into the fundamentals of enzyme technology and applied biocatalysis. The course covers all important steps of multidisciplinary process of enzyme technology development overlapping areas of cell and molecular biology, biochemistry and process engineering. The examples of succesfull “green” enzyme technology aplications are presented in the course.

At the end of this course, students should be able to: be familiar with the interdisciplinary approach required to utilize the catalytic potential of natural biomolecules, enzymes, or whole cells for the production of useful compounds understand the concept of sustainability and modern, environmentaly acceptable trends in industrial chemistry understand the basic principles of enzymatic reactions and when these catalysts are prefered have overview of reaction types, conditions and method for monitoring of biocatalytic proceses have knowledge how to obtain and optimise/improve enzyme catalysts select optimal biocatalyst (enzyme) for desired reaction design the basic biocatalytic process
Osnova
  • 1. Opening Lecture
  • 2. Introduction to Enzyme Technology
  • 3. Basics of Enzymes as Biocatalysts
  • 4. Kinetics of Enzyme Catalysed Reaction
  • 5. Principles and Application of Enzyme Selectivity
  • 6. Enzymes in Organic Synthesis I.
  • 7. Enzymes in Organic Synthesis II.
  • 8. Enzyme Production and Purification
  • 9. Engineering of Biocatalysts
  • 10. Immobilization of Enzymes and Cells
  • 11. Reaction Engineering and Process Technology
  • 12. Biotechnology Informatics
Literatura
  • BUCHHOLZ, Klaus, Volker KASCHE a Uwe BORNSCHEUER. Biocatalysts and enzyme technology. Weinheim: Wiley-VCH, 2005, xvii, 448. ISBN 3527304975. info
  • FABER, Kurt. Biotransformations in organic chemistry : a textbook. 5th rev. and corr. ed. Berlin: Springer-Verlag, 2004, xi, 454. ISBN 3540200975. info
Výukové metody
lectures with powerpoint presentation and pdf materials for printing, short training written tests at the beginning of each lecture and short exercises during lecture
Metody hodnocení
written test in English, 45 questions, maximum 45 points (successfulness/classification: >40/A, 30-39/B, 22-29/C, 16-21/D, 11-15/E, <11/F)
Vyučovací jazyk
Angličtina
Informace učitele
http://loschmidt.chemi.muni.cz/peg/
Další komentáře
Studijní materiály
Předmět je vyučován každoročně.
Předmět je zařazen také v obdobích podzim 2010 - akreditace, podzim 2008, podzim 2010, podzim 2011, podzim 2011 - akreditace.