C5150 Trendy v analytické chemii

Přírodovědecká fakulta
podzim 2019
Rozsah
2/0/0. 2 kr. (plus ukončení). Ukončení: zk.
Vyučující
prof. Mgr. Jan Preisler, Ph.D. (přednášející)
Garance
prof. Mgr. Jan Preisler, Ph.D.
Ústav chemie – Chemická sekce – Přírodovědecká fakulta
Dodavatelské pracoviště: Ústav chemie – Chemická sekce – Přírodovědecká fakulta
Rozvrh
Po 9:00–10:50 C14/207
Předpoklady
Passing courses Analytical chemistry I (C3100) and Analytical chemistry II (C4050).
Omezení zápisu do předmětu
Předmět je nabízen i studentům mimo mateřské obory.
Mateřské obory/plány
Cíle předmětu
During the course consisting of lectures given by experts of the Institute of Analytical Chemistry of Academy of Sciences of the Czech Republic students will learn advanced analytical techniques. The course also involves a visit of the Institute of Analytical Chemistry of ASCR.
Výstupy z učení
The course will widen students' knowledge of modern analytical chemistry. Students will visit a top scientific institution in the country.
Osnova
  • Outline of the Trends in Analytical Chemistry course
  • 21. 9. 2017 doc. RNDr. Michal Roth, CSc. Compressed fluids in analytical separations. Supercritical fluids and compressed liquids – similarities and differences, applications in chromatography and sample preparation, supercritical fluid chromatography, supercritical fluid extraction, pressurized hot water extraction. Ionic liquids – motivation, properties, applications. Supercritical water as an agent to treat fused silica capillaries.
  • 5. 10. 2017 Mgr. Markéta Vaculovičová, Ph.D. Nanoparticles in bioanalytical applications. List of nanoparticles used for in vitro and in vivo detection applications and imaging. Highlight of basic properties of various nanoparticle types, which are employed in bioanalysis and diagnostics.
  • 12. 10. 2017 doc. RNDr. Karel Šlais, DrSc. Preparative and analytical isoelectric focusing. Principles and variants of instrumental arrangement (capillary, gel, preparative channel), detection, staining, standards and pI-markers, applications in analysis of proteins and microorganisms, continuous methods, coupling to liquid chromatography and mass spectrometry. Current results – new electrolyte systems and focusing devices.
  • 19. 10. 2017 RNDr. Pavel Kubáň, Ph.D. DSc. Application of membrane-based pre-separation techniques in analysis of environmental, biological and clinical samples. New trends in treatment and pre-concentration of complex samples, examples of off-line and on-line couplings with current analytical instruments, examples of practical solutions of real-world problems.
  • 26. 10. 2017 Ing. František Foret, CSc. Principles of microfabrications for analytical instrumentation. Coupling of microseparation techniques to mass spectrometry.
  • 2. 11. 2017 doc. RNDr. Bohumil Dočekal, CSc. New approaches in atomic spectroscopy. Laboratories for trace analysis – “clean environment”.
  • 9. 11. 2017 Ing. Karel Klepárník, CSc. Current instruments for genetic research, medical diagnostics and molecular identification of organisms (principles of capillary electrophoresis and mass spectrometry for DNA analysis, separation media, laser-induced fluorescence, mutations and polydispersity of DNA, sample preparation for DNA analysis (cloning, restriction fission, DNA amplification by polymerase chain reaction, Sanger sequenation reaction), DNA sequenation, analysis of polymorphism of restriction fragments, ssDNA conformation polymorphism analysis, denaturation analysis, applications of capillary electrophoresis and mass spectrometry for medical diagnostics and molecular identification of organisms. Nanotechnology for genomic DNA sequenation.
  • 16. 11. 2017 doc. RNDr. Petr Kubáň, Ph.D. Analytical chemistry in point-of-care (POC) diagnostics. Definition of concepts, history, types of POC devices, examples. Indicator papers, lateral flow assays, paper-based microfluidics, portable analysers. Types of reactions, principles, detection. Example applications from our laboratory. New trends.
  • 23. 11. 2017 Mgr. Jana Křenková, Ph.D., Ing. Dana Moravcová, Ph.D. Monoliths in separation techniques. A short overview of monoliths employed in separation techniques with emphasis on synthesis, characterization and applications of monoliths in analysis of biological samples.
  • 30. 11. 2017 RNDr. Pavel Mikuška, CSc. Atmospheric aerosols. Basic definitions and terms, effects of aerosols on environment and human health, physical properties, aerosol sources, size distribution and concentration measurements, chemical composition, specific types of aerosols (bioaerosols, nanoaerosols, indoor aerosols), aerosol concentrations in the Czech Republic.
  • The course will terminate on December 7, 2017 with an excursion to the labs of The Institute of Analytical Chemistry of the Czech Academy of Sciences.
Literatura
  • Supplementary materials will be provided by the external lecturers
Výukové metody
Lectures and a visit of external workplace. The lectures are given in English.
Metody hodnocení
Final written examination
Vyučovací jazyk
Angličtina
Další komentáře
Studijní materiály
Předmět je zařazen také v obdobích podzim 2010 - akreditace, podzim 2009, podzim 2010, podzim 2011, podzim 2011 - akreditace, podzim 2012, podzim 2013, podzim 2014, podzim 2015, podzim 2016, podzim 2017, podzim 2018, podzim 2021, podzim 2023.