C9100 Biosensors

Faculty of Science
Autumn 2022
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Petr Skládal, CSc. (lecturer)
Guaranteed by
prof. RNDr. Petr Skládal, CSc.
Department of Biochemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science
Timetable
Mon 11:00–12:50 B11/335
Prerequisites
basic overview of biochemistry
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Students will gain overview of biosensors, including electrochemical and optical sensors. The experience on sensors based on affinity interactions as surface plasmon resonance (SPR) and piezoelectric transducers will serve for characterization of affinity interactions. The obtained knowledge will help to understand principles of the instrumentation available at the Faculty of Science and CEITEC MU. Students will learn applications of biosensors in clinical analysis, environmental monitoring, food production and security systems.
Learning outcomes
Student will learn principles of biocatalytic and affinity biosensors, properties of enzymes, antibodies and cells as biorecognition elements. Student will gain basic knowledge on the immobilization of biomolecules and applications of biosensors in clinical medicine, food industry, military and biotechnologies.
Syllabus
  • 1. Definition of the biosensor. Historic overview. Characteristics of ideal biosensors. Basic measuring techniques. 2. Electrochemical biosensors, enzyme electrodes. Potentiometric systems and ISFETs. Reference electrodes. 3. Amperometric measurement of oxygen, hydrogen peroxide and NADH, biosensors based on oxidases and dehydrogenases. 4. Electron transfer from enzymes to electrodes using mediators. Composite and organometallic electrodes. 5. Impedimetric and conductometric biosensors. Voltammetric techniques 6. Spectrophotometric, fluorimetric a chemiluminescent sensors, optical fibers. Optical biocatalytic sensors. Bioluminescence. 7. Biosensors for detection of inhibitors. Recycling enzyme systems. Microbial, tissue and receptor-based sensors. 8. Affinity biosensors based on indirect detection of labels. Immunosensors. 9. Hybridization biosensors for detection of nucleic acids and oligonucleotide sequences. 10. Direct optical affinity sensors. Evanescent wave and surface plasmon resonance utilized for monitoring of bioaffinity interactions in real time. Integrated optical systems, interferometers and related techniques. 11. Immobilization of biomolecules for construction of biosensors. Membrane techniques. Electropolymerization. 12. Activation of sensing surfaces. Covalent immobilization of biomolecules. 13. Miniaturization and mass production of biosensors. Screen-printing, litography and mass production of biosensors. Biosensors in integrated analytical microsystems. Biochips. 14. Commercial biosensors. Perspectives of biosensors, applications in clinical medicine, food production, environmental monitoring, military application.
Literature
  • VOET, Donald, Judith G. VOET and Charlotte W. PRATT. Fundamentals of biochemistry : life at the molecular level. 3rd ed. Hoboken: John Wiley & Sons, 2008, xxx, 1099. ISBN 9780470129302. info
Teaching methods
lecture course
Assessment methods
Participation at lectures required, oral examination - random selection of 3 questions from the subject, 10 min preparation of answers, discussion with the teacher, min. 50% of correct answers. Alternatively, the course might be finished as a colloquium.
Language of instruction
Czech
Further Comments
Study Materials
The course can also be completed outside the examination period.
The course is taught annually.
Teacher's information
http://www.sci.muni.cz/~skladal/
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 1999, Autumn 2010 - only for the accreditation, Autumn 2000, Autumn 2001, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2022/C9100