Curriculum Vitae

Curriculum Vitae

Person Identification

• Prof. Zbynek Prokop, Ph.D.
  born May 07, 1974 in Brno, Czech Republic

Affiliation and Address

• Loschmidt Laboratories, Institute of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno,Czech Republic

Professor

• Head, Laboratory of Kinetics and Microfluidics

Education and Academic Qualifications

• 2011: Prof., Environmental Chemistry, Masaryk University, Brno, Czech Republic
• 2011: Assoc. Prof., Environmental Chemistry, Masaryk University, Brno, Czech Republic
• 2001: Ph.D., Environmental Chemistry, Masaryk University, Brno, Czech Republic

Employment Summary

• 2000 – present: Faculty of Science, Masaryk University, Brno, Czech Republic
• 2013 – present: International Clinical Research Center, St. Anne's University Hospital Brno

Pedagogical Activities

• Bi7430 Molecular biotechnology
• Bi7430c Molecular biotechnology - practice
• C5855 Biophysical chemistry
• C7880 New Methods in Bioanalytical Chemistry
• Bi5111, Bi6009, Bi7623, Bi8624, Bi9625, Bi0626 Seminar of microbiology
• Bi9280 Summer school of protein engineering
• Bi7893 Seminar of Loschmidt laboratories
• Bi7894 Seminar of research teams in Loschmidt laboratories

Research Projects

• Enzyme Mechanism and Kinetics
• Enzyme-Based Technologies
• Protein Engineering
• Microfluidics

Academical Stays

• 4-5/2016: Technische Universität Wien, Austria
• 7-9/2014: ETH Zurich, Switzerland (EU-ERA Grant)
• 2/2012: University of Cambridge, United Kingdom
• 8/2011: University of Cambridge, United Kingdom
• 1/2010: Eötvös University, Hungary (EMBO Networking Grant)
• 2/2005: University of Hannover, Germany (NATO Collaborative Linkage Grant)
• 11/2004: EMBL Heidelberg, Germany (EMBO Networking Grant)
• 2-4/2004: University of Groningen, The Netherlands (EMBO Fellowship)
• 1-2/2002: University of Groningen, The Netherlands (NATO Science Fellowship)
• 10-11/2001: University of Groningen, The Netherlands (FEMS Fellowship)
• 3-5/1999: University of Ghent, Belgium (EU Socrates Student Exchange Program)

International Courses

• 2021: EMBO Laboratory Leadership Course
• 2017: EMBO Course of Transient Kinetics, University of Kent, United Kingdom
• 2016: Time-resolved Fluorescence Spectroscopy, Berlin, Germany
• 2016: Time-resolved microscopy and correlation spectroscopy, Berlin, Germany
• 2014: Course on modern enzyme kinetics, University of Texas, Austin, USA
• 2011: EMBO Laboratory Management Course, DE-Leimen, Germany
• 2008: EMBO Course of Transient Kinetic Methods, University of Kent, United Kingdom
• 2006: Advanced Course on Biocatalysis, Technical University of Delft, The Netherlands
• 2002: Course on CD-Spectrometry, University of Warwick, United Kingdom
• 2001: Course on CD-Spectrometry, University of Budapest, Hungary

Membership in Scientific Organizations

• Czech Society or Biochemistry and Molecular Biology
• Czech Chemical Society
• Czechoslovak Society of Microbiology
• Czech Society for Analytical Cytology

Appreciation by Scientific Community

• 2023: MUNI vice-rector's award for supervisors for excellent results in doctoral studies
• 2021: MUNI Innovation Award, Masaryk University
• 2020: MUNI Scientist Award, Masaryk University
• 2015: The best technology paper in 2014, Environmental Science & Technology, ACS
• 2015: Werner von Siemens Award for Excellence in Innovation, Siemens
• 2014: The Best Academy/Industry Collaboration of The Year, 2nd Price, American Chamber of Commerce and Czech Association for Foreign Investment
• 2008: J. V. Kostir Prize for Biochemistry, Czech Society of Biochememistry and Molecular Biology
• 2005: Alfred Bader Prize for Bioorganic Chemistry, Czech Chemical Society
• 2005: Excellence in Inovation Award of the Rector of Masaryk University
• 2002: Award for the Young Czech and Slovak Microbiologist of the Year, Czechoslovak Microbiological Society
• 2000: First place, Student Poster Competition, 5-th Internation Symposium on Environmental Contamination in Central and Eastern Europe

Selected Publications

• FRUHAUF, Sebastian, Dominic PUHRINGER, Michaela THAMHESL, Patricia FAJTL, Elisavet KUNZ-VEKIRU, Andreas HOBARTNER-GUSSL, Gerd SCHATZMAYR, Gerhard ADAM, Jiří DAMBORSKÝ, Kristina DJINOVIC-CARUGO, Zbyněk PROKOP and Wulf-Dieter MOLL. Bacterial Lactonases ZenA with Noncanonical Structural Features Hydrolyze the Mycotoxin Zearalenone. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2024, vol. 14, No 5, p. 3392-3410. ISSN 2155-5435. Available from: https://dx.doi.org/10.1021/acscatal.4c00271. URL info
• SLÁNSKÁ, Michaela, Lenka ŠTACKOVÁ, Sérgio Manuel MARQUES, Peter ŠTACKO, Marek MARTÍNEK, Luboš JÍLEK, Martin TOUL, Jiří DAMBORSKÝ, David BEDNÁŘ, Petr KLÁN and Zbyněk PROKOP. Azobenzene-Based Photoswitchable Substrates for Advanced Mechanistic Studies of Model Haloalkane Dehalogenase Enzyme Family. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2024, vol. 14, No 15, p. 11635-11645. ISSN 2155-5435. Available from: https://dx.doi.org/10.1021/acscatal.4c03503. URL info
• AKITA, Masaki, Paul GIRVAN, Mário ŠPÍREK, Jiří NOVÁČEK, David RUEDA, Zbyněk PROKOP and Lumír KREJČÍ. Mechanism of BCDX2-mediated RAD51 nucleation on short ssDNA stretches and fork DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2024, vol. 52, No 19, p. 11738-11752. ISSN 0305-1048. Available from: https://dx.doi.org/10.1093/nar/gkae770. URL info
• KUNKA, Antonín, Sérgio Manuel MARQUES, Martin HAVLÁSEK, Michal VAŠINA, Nikola VELÁTOVÁ, Lucia CENGELOVÁ, David KOVÁŘ, Jiří DAMBORSKÝ, Martin MAREK, David BEDNÁŘ and Zbyněk PROKOP. Advancing Enzyme's Stability and Catalytic Efficiency through Synergy of Force-Field Calculations, Evolutionary Analysis, and Machine Learning. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2023, vol. 13, No 19, p. 12506-12518. ISSN 2155-5435. Available from: https://dx.doi.org/10.1021/acscatal.3c02575. URL info
• TOUL, Martin, Dmitri NIKITIN, Martin MAREK, Jiří DAMBORSKÝ and Zbyněk PROKOP. Extended Mechanism of the Plasminogen Activator Staphylokinase Revealed by Global Kinetic Analysis: 1000-fold Higher Catalytic Activity than That of Clinically Used Alteplase. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2022, vol. 12, No 7, p. 3807-3814. ISSN 2155-5435. Available from: https://dx.doi.org/10.1021/acscatal.1c05042. URL info
• HESS, David, Veronika DOČKALOVÁ, Piia Pauliina KOKKONEN, David BEDNÁŘ, Jiří DAMBORSKÝ, Andrew DEMELLO, Zbyněk PROKOP and Stavros STAVRAKIS. Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling. Chem. Cambridge: Cell Press, 2021, vol. 7, No 4, p. 1066-1079. ISSN 2451-9294. Available from: https://dx.doi.org/10.1016/j.chempr.2021.02.011. URL info
• MAZURENKO, Stanislav, Zbyněk PROKOP and Jiří DAMBORSKÝ. Machine Learning in Enzyme Engineering. ACS Catalysis. Washington, D.C.: American Chemical Society, 2020, vol. 10, No 2, p. 1210-1223. ISSN 2155-5435. Available from: https://dx.doi.org/10.1021/acscatal.9b04321. URL info
• JINDAL, Garima, Kateřina SLÁNSKÁ, Veselin KOLEV, Jiří DAMBORSKÝ, Zbyněk PROKOP and Arieh WARSHEL. Exploring the challenges of computational enzyme design by rebuilding the active site of a dehalogenase. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: NATL ACAD SCIENCES, 2019, vol. 116, No 2, p. 389-394. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1804979115. URL info
• KOKKONEN, Piia Pauliina, J. SYKORA, Zbyněk PROKOP, A. GHOSE, David BEDNÁŘ, M. AMARO, Koen BEERENS, Šárka NEVOLOVÁ, Michaela SLÁNSKÁ, Jan BREZOVSKÝ, Jiří DAMBORSKÝ and M. HOF. Molecular Gating of an Engineered Enzyme Captured in Real Time. Journal of the American Chemical Society. Washington: American Chemical Society, 2018, vol. 140, No 51, p. 17999-18008. ISSN 0002-7863. Available from: https://dx.doi.org/10.1021/jacs.8b09848. URL info
• MAZURENKO, Stanislav, Antonín KUNKA, Koen BEERENS, Christopher M. JOHNSON, Jiří DAMBORSKÝ and Zbyněk PROKOP. Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating. Scientific Reports. London: NATURE PUBLISHING GROUP, 2017, vol. 7, November, p. nestránkováno, 14 pp. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-017-16360-y. URL info
• PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA and Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, vol. 49, No 35, p. 6111-6115. ISSN 1433-7851. URL info
• PROKOP, Zbyněk, Marta MONINCOVÁ, Radka CHALOUPKOVÁ, Martin KLVAŇA, Yuji NAGATA, Dick B. JANSSEN and Jiří DAMBORSKÝ. Catalytic mechanism of the haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26. Journal of Biological Chemistry. 2003, vol. 278, No 46, p. 45094-45100. ISSN 0021-9258. URL info

2025/01/05