Životopis

Curriculum Vitae

Person Identification

• Prof. Zbynek Prokop, Ph.D.
  born May 07, 1974 in Brno, Czech Republic

Affiliation and Address

• Loschmidt Laboratories, Institute of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno,Czech Republic

Professor

• Head, Laboratory of Kinetics and Microfluidics

Education and Academic Qualifications

• 2011: Prof., Environmental Chemistry, Masaryk University, Brno, Czech Republic
• 2011: Assoc. Prof., Environmental Chemistry, Masaryk University, Brno, Czech Republic
• 2001: Ph.D., Environmental Chemistry, Masaryk University, Brno, Czech Republic

Employment Summary

• 2000 – present: Faculty of Science, Masaryk University, Brno, Czech Republic
• 2013 – present: International Clinical Research Center, St. Anne's University Hospital Brno

Pedagogical Activities

• Bi7430 Molecular biotechnology
• Bi7430c Molecular biotechnology - practice
• C5855 Biophysical chemistry
• C7880 New Methods in Bioanalytical Chemistry
• Bi5111, Bi6009, Bi7623, Bi8624, Bi9625, Bi0626 Seminar of microbiology
• Bi9280 Summer school of protein engineering
• Bi7893 Seminar of Loschmidt laboratories
• Bi7894 Seminar of research teams in Loschmidt laboratories

Research Projects

• Enzyme Mechanism and Kinetics
• Enzyme-Based Technologies
• Protein Engineering
• Microfluidics

Academical Stays

• 4-5/2016: Technische Universität Wien, Austria
• 7-9/2014: ETH Zurich, Switzerland (EU-ERA Grant)
• 2/2012: University of Cambridge, United Kingdom
• 8/2011: University of Cambridge, United Kingdom
• 1/2010: Eötvös University, Hungary (EMBO Networking Grant)
• 2/2005: University of Hannover, Germany (NATO Collaborative Linkage Grant)
• 11/2004: EMBL Heidelberg, Germany (EMBO Networking Grant)
• 2-4/2004: University of Groningen, The Netherlands (EMBO Fellowship)
• 1-2/2002: University of Groningen, The Netherlands (NATO Science Fellowship)
• 10-11/2001: University of Groningen, The Netherlands (FEMS Fellowship)
• 3-5/1999: University of Ghent, Belgium (EU Socrates Student Exchange Program)

International Courses

• 2021: EMBO Laboratory Leadership Course
• 2017: EMBO Course of Transient Kinetics, University of Kent, United Kingdom
• 2016: Time-resolved Fluorescence Spectroscopy, Berlin, Germany
• 2016: Time-resolved microscopy and correlation spectroscopy, Berlin, Germany
• 2014: Course on modern enzyme kinetics, University of Texas, Austin, USA
• 2011: EMBO Laboratory Management Course, DE-Leimen, Germany
• 2008: EMBO Course of Transient Kinetic Methods, University of Kent, United Kingdom
• 2006: Advanced Course on Biocatalysis, Technical University of Delft, The Netherlands
• 2002: Course on CD-Spectrometry, University of Warwick, United Kingdom
• 2001: Course on CD-Spectrometry, University of Budapest, Hungary

Membership in Scientific Organizations

• Czech Society or Biochemistry and Molecular Biology
• Czech Chemical Society
• Czechoslovak Society of Microbiology
• Czech Society for Analytical Cytology

Appreciation by Scientific Community

• 2023: MUNI vice-rector's award for supervisors for excellent results in doctoral studies
• 2021: MUNI Innovation Award, Masaryk University
• 2020: MUNI Scientist Award, Masaryk University
• 2015: The best technology paper in 2014, Environmental Science & Technology, ACS
• 2015: Werner von Siemens Award for Excellence in Innovation, Siemens
• 2014: The Best Academy/Industry Collaboration of The Year, 2nd Price, American Chamber of Commerce and Czech Association for Foreign Investment
• 2008: J. V. Kostir Prize for Biochemistry, Czech Society of Biochememistry and Molecular Biology
• 2005: Alfred Bader Prize for Bioorganic Chemistry, Czech Chemical Society
• 2005: Excellence in Inovation Award of the Rector of Masaryk University
• 2002: Award for the Young Czech and Slovak Microbiologist of the Year, Czechoslovak Microbiological Society
• 2000: First place, Student Poster Competition, 5-th Internation Symposium on Environmental Contamination in Central and Eastern Europe

Selected Publications

• FRUHAUF, Sebastian, Dominic PUHRINGER, Michaela THAMHESL, Patricia FAJTL, Elisavet KUNZ-VEKIRU, Andreas HOBARTNER-GUSSL, Gerd SCHATZMAYR, Gerhard ADAM, Jiří DAMBORSKÝ, Kristina DJINOVIC-CARUGO, Zbyněk PROKOP a Wulf-Dieter MOLL. Bacterial Lactonases ZenA with Noncanonical Structural Features Hydrolyze the Mycotoxin Zearalenone. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2024, roč. 14, č. 5, s. 3392-3410. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.4c00271. URL info
• SLÁNSKÁ, Michaela, Lenka ŠTACKOVÁ, Sérgio Manuel MARQUES, Peter ŠTACKO, Marek MARTÍNEK, Luboš JÍLEK, Martin TOUL, Jiří DAMBORSKÝ, David BEDNÁŘ, Petr KLÁN a Zbyněk PROKOP. Azobenzene-Based Photoswitchable Substrates for Advanced Mechanistic Studies of Model Haloalkane Dehalogenase Enzyme Family. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2024, roč. 14, č. 15, s. 11635-11645. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.4c03503. URL info
• AKITA, Masaki, Paul GIRVAN, Mário ŠPÍREK, Jiří NOVÁČEK, David RUEDA, Zbyněk PROKOP a Lumír KREJČÍ. Mechanism of BCDX2-mediated RAD51 nucleation on short ssDNA stretches and fork DNA. Nucleic acids research. Oxford: Oxford University Press, 2024, 15 s. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkae770. URL info
• KUNKA, Antonín, Sérgio Manuel MARQUES, Martin HAVLÁSEK, Michal VAŠINA, Nikola VELÁTOVÁ, Lucia CENGELOVÁ, David KOVÁŘ, Jiří DAMBORSKÝ, Martin MAREK, David BEDNÁŘ a Zbyněk PROKOP. Advancing Enzyme's Stability and Catalytic Efficiency through Synergy of Force-Field Calculations, Evolutionary Analysis, and Machine Learning. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2023, roč. 13, č. 19, s. 12506-12518. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.3c02575. URL info
• TOUL, Martin, Dmitri NIKITIN, Martin MAREK, Jiří DAMBORSKÝ a Zbyněk PROKOP. Extended Mechanism of the Plasminogen Activator Staphylokinase Revealed by Global Kinetic Analysis: 1000-fold Higher Catalytic Activity than That of Clinically Used Alteplase. ACS Catalysis. WASHINGTON: AMER CHEMICAL SOC, 2022, roč. 12, č. 7, s. 3807-3814. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.1c05042. URL info
• HESS, David, Veronika DOČKALOVÁ, Piia Pauliina KOKKONEN, David BEDNÁŘ, Jiří DAMBORSKÝ, Andrew DEMELLO, Zbyněk PROKOP a Stavros STAVRAKIS. Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling. Chem. Cambridge: Cell Press, 2021, roč. 7, č. 4, s. 1066-1079. ISSN 2451-9294. Dostupné z: https://dx.doi.org/10.1016/j.chempr.2021.02.011. URL info
• MAZURENKO, Stanislav, Zbyněk PROKOP a Jiří DAMBORSKÝ. Machine Learning in Enzyme Engineering. ACS Catalysis. Washington, D.C.: American Chemical Society, 2020, roč. 10, č. 2, s. 1210-1223. ISSN 2155-5435. Dostupné z: https://dx.doi.org/10.1021/acscatal.9b04321. URL info
• JINDAL, Garima, Kateřina SLÁNSKÁ, Veselin KOLEV, Jiří DAMBORSKÝ, Zbyněk PROKOP a Arieh WARSHEL. Exploring the challenges of computational enzyme design by rebuilding the active site of a dehalogenase. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: NATL ACAD SCIENCES, 2019, roč. 116, č. 2, s. 389-394. ISSN 0027-8424. Dostupné z: https://dx.doi.org/10.1073/pnas.1804979115. URL info
• KOKKONEN, Piia Pauliina, J. SYKORA, Zbyněk PROKOP, A. GHOSE, David BEDNÁŘ, M. AMARO, Koen BEERENS, Šárka NEVOLOVÁ, Michaela SLÁNSKÁ, Jan BREZOVSKÝ, Jiří DAMBORSKÝ a M. HOF. Molecular Gating of an Engineered Enzyme Captured in Real Time. Journal of the American Chemical Society. Washington: American Chemical Society, 2018, roč. 140, č. 51, s. 17999-18008. ISSN 0002-7863. Dostupné z: https://dx.doi.org/10.1021/jacs.8b09848. URL info
• MAZURENKO, Stanislav, Antonín KUNKA, Koen BEERENS, Christopher M. JOHNSON, Jiří DAMBORSKÝ a Zbyněk PROKOP. Exploration of Protein Unfolding by Modelling Calorimetry Data from Reheating. Scientific Reports. London: NATURE PUBLISHING GROUP, 2017, roč. 7, November, s. nestránkováno, 14 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/s41598-017-16360-y. URL info
• PROKOP, Zbyněk, Yukari SATO, Jan BREZOVSKÝ, Tomáš MOZGA, Radka CHALOUPKOVÁ, Táňa KOUDELÁKOVÁ, Petr JEŘÁBEK, Veronika ŠTĚPÁNKOVÁ, Rio NATSUME, J.G.E. LEEWEN, D,B. JANSSEN, J. FLORIAN, Yuji NAGATA, T. SENDA a Jiří DAMBORSKÝ. Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering. Angewandte Chemie International Edition. Wiley-VCH Verlag GmbH & Co. KGaA, Weinhe, 2010, roč. 49, č. 35, s. 6111-6115. ISSN 1433-7851. URL info
• PROKOP, Zbyněk, Marta MONINCOVÁ, Radka CHALOUPKOVÁ, Martin KLVAŇA, Yuji NAGATA, Dick B. JANSSEN a Jiří DAMBORSKÝ. Catalytic mechanism of the haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26. Journal of Biological Chemistry. 2003, roč. 278, č. 46, s. 45094-45100. ISSN 0021-9258. URL info

2025/01/05