Curriculum Vitae

Curriculum vitae

Name, first names, scientific degrees

• Vrabel Patrik, Mgr.
  date of birth November 27th, 1975 in Olomouc, Moravia, Czech Republic.

Department/Faculty/University

• Department of Analytical Chemistry,
  Faculty of Science, Masaryk University,
  Kotlarska 2, 61137 Brno, Czech Republic.

Function, current position

• Ph.D. fulltime student.

Education and academic qualifications

• 2003 - present: Doctoral degree, Analytical Chemistry, expected graduation in June 2006.
  Title of Doctoral Thesis: The Proteome Study of Bacteriophage B812 Using Capillary Electrophoresis and Mass Spectrometry.
  Masaryk University, Faculty of Science, Supervisor: Josef Havel, Scientific Supervisor: Jan Preisler.
• 1997 - 2003: Masters degree, Analytical Chemistry and Didactics of Chemistry, Mgr.
  Title of Diploma Thesis: Combination of Capillary Electrophoresis with Mass Spectrometry for Analysis of Peptides.
  Masaryk University, Faculty of Science, Supervisor: Jan Preisler.

Research experience

• Chemistry instrumentation development
  In cooperation with my supervisor and technician in our department I constructed and tested 532 nm laser induced fluorescence detection system for Capillary Electrophoresis. I was responsible to measurement, improvement and service of CELIF and CEUV instrument for 5 years. I gained practical knowledge about setting optics, photomultiplier tubes (PMT) applications, solid state lasers application, fluorescence measurements and data aquisition throughout I/O digitizing PC card.
  During my Ph.D. study I constructed and tested subatmospheric pressure deposition device for coupling CE separation with MALDI MS detection. I gained practical knowledge about microfluidic technology, coupling CE to MS, crystallizing process and vacuum technology.
  I learned application programming for PMT data aquisition and driving stepper motors in NI LabVIEW 6.1 software.
• Proteomic Analysis
  Aim of my Ph.D. thesis is an alternative proteomics analysis of bacteriophage 812 proteins utilizing developed platform mentioned above (protein fraction>CE-deposition>on-target LIF identification>on-target digestion>MS). The obtained proteomic data are evaluated to genome database, gained proteome map is compared to classic proteomic approach (protein fraction>PAGE>digestion in solution>MS). The main advantages of the alternative approach are higher sensitivity, very low consumption of a sample and complementary information output to the classic approach.
• Methodology
  Column Separation: I gained experience with CE, ITP and HPLC separation of rhodamine based dyes, aminoacids, peptides and proteins, nucleotides and fluorescent derivatized biomolecules containing amino groups.
  Fluorescence: Measuring and detection of fluorescence in-solution or in-solid state after excitation with Xe lamp or 532/266 nm solid state lasers. I studied effect of organic solvents/water electrolytes to fluorescence in solution and effect of additives in case of solid state fluorescence.
  Mass Spectrometry: I have 5 years experience with MALDITOF MS measurements of peptides and proteins, rhodamine based dyes derivatized peptides & proteins and digested proteins. Automation of mass spectrometric measurement & data processing.

Teaching activities

• "Informatics - basic" and "Informatics - computer application for chemists".
• "Advanced Laboratory Course" in CELIF and CE-MS laboratory.
• Full-time teaching of "Chemistry" on high school during study of Didactics of Chemistry.
• Teaching of chemistry, informatics and biology at an elementary school from September 2006.

Research activities, projects, grants

• 2002 - 2003: Grant Project of Czech Ministry of Youth, Sport and Education #793/2002.
  Title: Capillary Electrophoresis with Laser Induced Fluorescence (LIF) Detection System for Advanced Laboratory Course.
  Applicant: Jan Preisler, Co-Applicants: Josef Havel, Patrik Vrabel.
  State: successfully resolved.
• 2003 - 2005: Grant Project of Grant Agency of the Czech Republic #203/03/0515.
  Title: Integrated Analysis of Genome and Proteome of Therapeutically Important Bacteriophages.
  Applicant: Jan Preisler, Co-Applicants: Karel Kleparnik.
  State: on-going.
• 2005: Grant Project of Czech Ministry of Youth, Sport and Education #2897/2005.
  Title: Study of Derivatization of Peptides with Fluorescent Reagent Using Matrix-Assisted Laser Desorption/Ionization Spectrometry.
  Applicant: Patrik Vrabel, Co-Applicants: Jan Preisler.
  State: successfully resolved.

Professional stays abroad

• International Erasmus intensive programme for teachers - Teaching in Foreign Languages:
  TiFoLa 2, January 8-22, 2005 in Brno, Faculty of Didactics, Masaryk University, Czech Republic.
  TiFoLa 3, January 8-22, 2006 in Limerick, Mary Immaculate College, Ireland.

Other academic activities, conferences, talks

• 53rd Annual ASMS Conference (American Society for Mass Spectrometry), San Antonio, Texas, June 5-9, 2005.
  Poster #1 Title: Capillary Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Native Fluorescence Detection for Protein Analysis.
  Poster #2 Title: Study of Derivatization of Peptides and Proteins for Fluorescence Detection Using Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.
• Biologically Active peptides IX, Prague, Czech Republic, April 20-22, 2005.
  Talk Title: Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection.
• 3rd International and 28th European Peptide Symposium, Prague, Czech Republic, September 5-10, 2004.
  Poster Title: Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Fluorescently Labeled Peptides for Optimization of Laser Induced Fluorescence Detection.
• 17th International Symposium on Microscale Separation and Capillary Electrophoresis, Salzburg, Austria, February 8-12, 2004.
  Poster Title: Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry.
• 54th Meeting of Czech Chemical Society, Brno, Czech Republic, July 1-4, 2002.
  Poster Title: Development and Characterization of Capillary Electrophoresis Instrument Utilizing Laser Induced Fluorescence Detection System.

Major publications

• VRÁBEL, Patrik, Petr TÁBORSKÝ, Markéta RYVOLOVÁ, Josef HAVEL and Jan PREISLER. Sensitive Detection and Separation of Fluorescent Derivatives using Capillary Electrophoresis with Laser-Induced Fluorescence Detection with 532 nm Nd:YAG Laser. Journal of Luminescence. Holland: Elsevier, 2006, vol. 118, No 2, p. 283-292. ISSN 0022-2313. URL info
• RYVOLOVÁ, Markéta, Petr TÁBORSKÝ, Patrik VRÁBEL, Josef HAVEL and Jan PREISLER. Derivatizace aminokyselin, peptidů a proteinů pro laserem indukovanou fluorescenční detekci v kapilární elektroforéze (Derivatization of Amino Acids, Peptides and Proteins for Laser-Induced Fluorescence Detection in Capillary Electrophoresis). Chemické listy. Praha: Asociace českých chemických společností, 2006, vol. 100, No 3, p. 191-195, 6 pp. ISSN 0009-2770. URL info
• PREISLER, Jan, Patrik VRÁBEL, Ondřej PEŠ, Helena ŘEHULKOVÁ and Josef HAVEL. Capillary Electrophoresis Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Native Fluorescence Detection for Protein Analysis. In Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied topics. 2005. info
• VRÁBEL, Patrik, Petr TÁBORSKÝ, Markéta RYVOLOVÁ, Helena ŘEHULKOVÁ, Josef HAVEL and Jan PREISLER. Study of Fluorescently Labelled Peptides and Small Proteins Using Capillary Electrophoresis Combined with Laser Induced Fluorescence and MALDI MS Detection. In Collection Symposium Series, Biollogicaly Active Peptides. 8th ed. Praha: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, 2005, p. 103-106. ISBN 80-86241-26-2. URL info
• VRÁBEL, Patrik, Jan PREISLER, Markéta RYVOLOVÁ, Helena ŘEHULKOVÁ and Petr TÁBORSKÝ. Study of Derivatization of Peptides and Proteins for Fluorescence Detection Using Capillary Electrophoresis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. In Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied topics. 2005. URL info
• PREISLER, Jan, Patrik VRÁBEL, Jaromíra NOVÁKOVÁ, Michal SPĚŠNÝ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Pavel KRÁSENSKÝ, Luděk EYER, Jiří DOŠKAŘ, Roman PANTŮČEK, František FORET and Karel KLEPÁRNÍK. Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry. In Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis. 2004. URL info
• VRÁBEL, Patrik, Petr TÁBORSKÝ, Markéta RYVOLOVÁ, Josef HAVEL and Jan PREISLER. Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Fluorescently Labeled Peptides for Optimization of Laser Induced Fluorescence Detection. In Journal of Peptide Science, 3rd International and 28th European PEPTIDE Symposium. Supplement to Vol. 10. Prague, Czech Republic: WILEY, 2004, p. 282. ISBN 965-90833-0-0. URL info
• VRÁBEL, Patrik, Jan PREISLER, Josef HAVEL and Pavel KRÁSENSKÝ. Vývoj a charakterizace přístroje pro kapilární elektroforézu s laserem indukovanou fluorescenční detekcí (Development and Characterization of a Capillary Electrophoresis Instrument with Laser-Induced Fluorescence Detection). In Chemické listy, Sborník. ročník 96, vydání 6. Praha: Česká společnost chemická, 2002, p. 459-460. ISSN 0009-2770. info

2006/11/07

Another Variant: Czech(1)