BLIM0411c Clinical Immunology - practice

Faculty of Medicine
spring 2025
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
prof. MUDr. Jiří Litzman, CSc. (lecturer)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor), Mgr. Julie Štíchová (deputy)
RNDr. Jana Nechvátalová, Ph.D. (seminar tutor)
Mgr. Peter Slanina (seminar tutor)
Mgr. Julie Štíchová (seminar tutor)
Mgr. Marcela Vlková, Ph.D. (seminar tutor)
Mgr. Martina Vráblíková (assistant)
Guaranteed by
Mgr. Marcela Vlková, Ph.D.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Miroslava Bučková
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Prerequisites
BLFY0222p Physiology II-lec.
Completion of subjects BLFY0222p
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The practical course is are focussed to methods used in laboratory diagnostics of immunopathological states, to indication and interpretation of laboratory immunological test and diagnostic algorithms of immunopathological diseases.
Learning outcomes
After finishing the course the student will be able to apply and interpret basic immunological laboratory tests. The sudents must manage laboraty tests protocol preparation of the tests used.
Syllabus
  • Antigens, immunization, serum, antisera preparation.
  • Monoclonal antibodies.
  • Principles of reaction of antigen plus antiboidy.
  • Agglutination.
  • Precipitation in gel and solution. Immunoelectrophoresis, immunofixation, nephelometry, turbidimetry.
  • Immunoassays, imunofluorescence, ELISA, RIA, LIA.
  • Isolation of the cells of the immune system from blood and other biological material.
  • Flow cytometry.
  • Lymphocyte cultivation in vivo, proliferation tests, cytokine production. Tests for in-vitro production of antibodies, ELISPOT.
  • Phagocytic tests.
  • Complement system evaluation.
  • Good laboratory practice.
Literature
  • LITZMAN, Jiří, Zita CHOVANCOVÁ, Vojtěch THON, Marcela VLKOVÁ, Vlastimil KRÁL and Tomáš FREIBERGER. Základy vyšetření v klinické imunologii (Investigations in clinical immunology). 2., přepr. vyd. Brno: Masarykova univerzita, 2015, 71 pp. ISBN 978-80-210-7853-6. info
  • Vyšetřovací metody v imunologii. Edited by Jiřina Bartůňková - Milan Paulík. 2., přeprac. a dopl. vyd. Praha: Grada, 2011, 164 s. ISBN 9788024735337. info
Teaching methods
Laboratory practice. Home preparation for the practice is required.
Assessment methods
To obtain the credit presence in 90% of the lectures is required. The quality of laboratory protocols is assessed.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2007, Spring 2008, Autumn 2008, Spring 2009, Autumn 2009, Spring 2010, Autumn 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.
  • Enrolment Statistics (spring 2025, recent)
  • Permalink: https://is.muni.cz/course/med/spring2025/BLIM0411c