LF:VLMB011 Imaging and analytical methods - Course Information

VLMB011 Modern imaging and analytical methods in cellular biology

Extent and Intensity

0/0/.6. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching

Teacher(s)

Mgr. Jan Frič, Ph.D., MHA (lecturer)
Mgr. Kamila Bendíčková, Ph.D. (lecturer)
Mgr. Marcela Hortová Kohoutková, Ph.D. (lecturer)
Mgr. Petra Lázničková, Ph.D. (lecturer)
Mgr. Veronika Bosáková (seminar tutor)
Mgr. Filip Kafka (seminar tutor)
Ioanna Papatheodorou, MSc (seminar tutor)
Mgr. Zuzana Tomášiková (seminar tutor)
Bc. Gabriela Blažková (seminar tutor)

Guaranteed by

Mgr. Jan Frič, Ph.D., MHA
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Zuzana Tomášiková
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine

Timetable

Fri 1. 11. 9:00–15:00 Virtuální místnost LF/Virtual room LF, Fri 15. 11. 9:00–15:00 A 1.08

Prerequisites

VLBI0222p Biology II-lect. || ZLBI0222p Biology II-lect. || PřF:Bi1700 Cell Biology || VSBI0222p Biology II-lect.
The prerequisite is knowledge of cell biology at the first-year university level, demonstrated by passing an exam (VLBI0222p, ZLBI0222p, or Bi1700).

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 15 student(s).
Current registration and enrolment status: enrolled: 6/15, only registered: 0/15

fields of study / plans the course is directly associated with

• General Medicine (programme LF, M-VL) (2)
• Dentistry (programme LF, M-ZL) (2)

Course objectives

After completing the course, the student will gain an overview of the currently used methods for imaging and analyzing cells and molecules and will become practically familiar with some of their applications. The student will visit laboratories and become acquainted with instruments for visualizing biomolecules in cells and tissues (cell imaging), such as fluorescence microscopes with confocal imaging, flow cytometry, and other imaging techniques. The student will understand the principles and applications of these methods, gain a deeper understanding of modern biological studies, develop an idea of how these methods are used in biomedical research and modern medicine, and be able to perform basic interpretation of results obtained through these methods.

Learning outcomes

Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.

Syllabus

• Friday, November 1, 2024:
• Online lectures from 9:00 AM to 3:00 PM (MS Teams).
• Lectures will be recorded and made available in the study materials, but attendance at the online lecture is highly recommended.
• Topics:
• fluorescent labeling and confocal microscopy,
• principles of flow cytometry and its applications,
• cell sorting using flow cytometry,
• advanced cytometric techniques (spectral and mass cytometry),
• advanced microscopy techniques,
• other methods for the analysis and visualization of biomolecules.
• Friday, November 15, 2024 - Mandatory attendance:
• Meeting at the Biology Park (near the University Campus Bohunice)
• Morning session 9:00 AM - 11:15 AM:
• Tour of the laboratory with demonstrations of the discussed methods directly in the lab.
• Afternoon session 12:00 PM - 1:45 PM:
• Application of imaging and analytical methods in biomedical research.
• Final test 2:00 PM:
• 2 open-ended questions from each discussed method covering the basic principles of the methods.
• Contact person: zuzana.tomasikova@fnusa.cz

Literature

recommended literature
• prezentace z přednášek

Teaching methods

seminar, lab demonstration

Assessment methods

Course completion requires attendance and passing a final written test (10 questions about the basic principles of demonstrated methods).
Attendance at an online seminar is highly recommended; in-person seminars require full attendance. Attendance at all seminars is mandatory

Language of instruction

Czech

Further comments (probably available only in Czech)

The course is taught annually.
Information on the extent and intensity of the course: 10.
Information on course enrolment limitations: Pokud nemá student zkoušku z Biologie II, musí mít absolvovaný předmět Bunečná biologie Bi1700 na PřF.

Teacher's information

https://is.muni.cz/auth/predmet/med/podzim2024/VLMB011
The teaching takes place in blocks on two Fridays (1.11. a 15.11.2024).

• Enrolment Statistics (recent)
  
• Permalink: https://is.muni.cz/course/med/autumn2024/VLMB011