BLLM0321c Medical Microbiology I - practice

Faculty of Medicine
Autumn 2009
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
Michaela Studýnková (seminar tutor), MUDr. Ondřej Zahradníček (deputy)
Alena Svobodová (seminar tutor), MUDr. Ondřej Zahradníček (deputy)
Michaela Gregorovičová (assistant)
Iva Holešovská (assistant)
Guaranteed by
prof. MUDr. Miroslav Votava, CSc.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Timetable
Fri 7:00–9:30 MiÚ N02104, Fri 11:10–13:40 MiÚ N02104
Prerequisites
BLMB011p Biology - lecture
Basic knowledge of laboratory work expected.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to practically use following methods in medical microbiology:
microscopy (staining, work with microscoope)
culture (inoculation, use of different media)
identification of bacteria and yeasts
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Syllabus
  • 1. Safety and health protection in microbiological laboratory. Microscopy - wet mount, simple staining
  • 2. Diagnostic Gram staining
  • 3. Staining of capsulla, review
  • 4. Culure media, preparing blood agar
  • 5. Conditions of culture, blood agar culturing, growth of bacteria on culture media
  • 6. Evaluation of cultures, biochemical identification tests
  • 7. Antibiotic susceptibility testing - disc tests, MIC demostration, E-test demonstration
  • 8. Animal experiment
  • 9. Introduction to serology - reactions, serum dilution, titer counting, pipetting
  • 10. Slide agglutiation, agglutination on carriers
  • 11. Agglutination in test tubes, Widal reaction
  • 12. Serologic reactions – AKE, RRR
  • 13. Complementfixing test
  • 14. Neutralisation – ASO
  • 15. ELISA
  • (16.–30. see BLLM0322c)
Literature
  • VOTAVA, Miroslav, Vlastimil OBDRŽÁLEK, Petr ONDROVČÍK, Filip RŮŽIČKA, Ondřej ZAHRADNÍČEK and Vladana WOZNICOVÁ. Lékařská mikrobiologie II. Přehled vyšetřovacích metod v lékařské mikrobiologii (Medical Microbiology II. Survey of Diagnostic Methods in Medical Microbiology). 1st ed. Brno: Masarykova univerzita. Lékařská fakulta, 2000, 309 pp. ISBN 80-210-2272-8. info
  • Prezentace z přednášek
Teaching methods
practical training
Assessment methods
For credit, it is necessary:
(1) to absolve all preactical sessions, but:
- two absences, either justified of approved by a teacher are possible; even so student has to demonstrate completing the missing topics
- more absences should be substituted
- not justified absences are not tolerated
(2) complete report of all practicals
(3) succesfully written credit test, eventually also other current tests; information about all tests will be given in advance.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
Information on completion of the course: Podmínky zápočtu jsou uvedeny v Doplňkových údajích k předmětu
The course is taught annually.
Listed among pre-requisites of other courses
Teacher's information
http://www.medmicro.info
The course is also listed under the following terms Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (Autumn 2009, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2009/BLLM0321c