BLAT051 Applicated analytical and instrumental techniques in laboratory medicine

Faculty of Medicine
Autumn 2011
Extent and Intensity
3/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslava Beňovská, Ph.D. (lecturer)
RNDr. Petr Breinek (lecturer)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
prof. RNDr. Zdeněk Glatz, CSc. (lecturer)
Mgr. Jana Gottwaldová (lecturer)
doc. RNDr. Oldřich Janiczek, CSc. (lecturer)
RNDr. Alena Mikušková (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Timetable
Tue 14:30–16:10 C05/107
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of the theoretical preparation part is to provide a theoretical base of physical principles used in analytical and laboratory technology. To familiarize students in detail with the application of these physical principles in specific instruments including existing modifications in contemporary medical laboratory equipment. To observe variations and modifications of specific examples and determine their purpose, advantages and critical sections in seminars, demonstrations and practical exercises.; A student will be able to do the following upon completing the course: Have an overview of new instrumental technologies; Understand the technical parameters of laboratory instruments and be able to compare them with one another; Judge the advantages, disadvantages and limitations of individual instrumental and analytical systems; Apply acquired theoretical knowledge to practical work in a laboratory; Follow standard technical operational procedures;
Syllabus
  • Analytical and perianalytical atomation and robotization: technical solutions, IT support,LIS/HIS,teleadministration, communications standards
  • Oint of care testing: applications in patients,technical recruiments, teleadministration, laboratory supersision,
  • Electrophoretic separations technics: agarophoresis, isoelectric focusation,cappilary electrophoresis, immunofixation,2D electrophorese
  • Chromatography:TLC-drugs, LC, HPLC, GC, MS/MS-tandem
  • Atomi absorption sepektrometry: phlame and electrothermal atomisation, background corrections,optimalisation
  • ELISA: principals of medical applications
  • Chemiluminiscence, electrochemiluminiscence, immunochemical automatic analyzers
  • FPIA, MEI:immunochemical automatic analyzers
  • DELFIA: medical applications
  • Blood gases and acidbase analyzers
  • Ionselective electrodes, biosensores
  • Potenciometry, conductometry, voltametry
  • Flow-cytometry: applications in clinical hematology and biochemistry
  • Osmometry: kryoscopic and ebulioscopic principals
  • Molecular and biological technics
Literature
  • Clinical chemistry : techniques, principles, correlations. Edited by Michael L. Bishop - Edward P. Fody - Larry E. Schoeff. 6th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins, 2010, xx, 732. ISBN 9780781790451. info
  • Tietz fundamentals of clinical chemistry. Edited by Carl A. Burtis - Edward R. Ashwood. 5th ed. Philadelphia: W.B. Saunders Company, 2001, xxv, 1091. ISBN 0721686346. info
  • DASTYCH, Milan. Instrumentální technika : Obor zdravotní laborant. 1. dotisk 1. vyd. Brno: Masarykova univerzita, 2010, 131 pp. ISBN 978-80-210-4226-1. info
  • KOMÁREK, Josef. Atomová absorpční spektrometrie. 1. vyd. Brno: Masarykova univerzita, 2000, 85 s. ISBN 802102500X. info
Teaching methods
a lecture with the use of audiovisual teaching aids (pp-presentations, commented videos) including interactive input;
Assessment methods
oral exam, written test, multiple choise test
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
The course is also listed under the following terms Autumn 2012, Autumn 2013, Autumn 2014.
  • Enrolment Statistics (Autumn 2011, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2011/BLAT051