BLKB0321c Clinical Biochemistry I - practice

Faculty of Medicine
autumn 2018
Extent and Intensity
0/2/0. 5 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
RNDr. Miroslava Beňovská, Ph.D. (seminar tutor)
RNDr. Hana Dobrovolná (seminar tutor)
RNDr. Pavla Hložková (seminar tutor)
Ing. Martina Podborská, Ph.D. (seminar tutor)
Mgr. Jana Tomanová (seminar tutor)
Mgr. Ondřej Wiewiorka, Ph.D. (seminar tutor)
Petr Coufal (assistant)
Michaela Gregorovičová (assistant)
Bc. Dana Peňázová (assistant)
Eva Střítecká (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Timetable of Seminar Groups
BLKB0321c/01: Wed 13:30–15:10 N04902
BLKB0321c/02: Wed 13:30–15:10 N05079
BLKB0321c/03: Wed 13:30–15:10 N05079
Prerequisites
BLBC0211c Biochemistry -p. && BLBC0211s Biochemistry -sem. && BLIT0222p Instrument Technology II - l.
completion of subject Biochemistry BLBC0211c && BLBC0211s && BLIT0222p
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of the theoretical preparation part is to provide detailed information about methods that utilize the latest knowledge in the field with regard to the contemporary level of routine use of new procedures and technologies. The basics of indication and interpretation of laboratory procedures with regard to clinical units and diagnoses. In the practical preparation part, the goal is to gain practical skills on a level that will allow the graduates to easily be included in clinical laboratory work teams.;
Learning outcomes
A student will be able to do the following upon completing the course: Perform and verify analytical methods and release biochemical findings; Determine the correctness of extra-laboratory preanalytical procedures; Evaluate the quality of an analytical sample; Use the laboratory information system; Know and adhere to the policies of correct laboratory work during the preanalytical, analytical and postanalytical process;
Syllabus
  • Practical skills; Reception and identification of biological material-paper and electronic order form, barcodes, barcode readers and printers; Laboratory information systems, on-line and off-line communication, pipeting list, printing output, supervise of results, monitoring of economy; Preparation of analytical sample, tubes, labeling, centrifugation, aliquoting, sorting; Automatic biochemistry analyzers, chemical parameters of methods, preparation of reagens, monitoring of analytical reactions, lag-phase, linearity, substrate consumption; Chemical and morfological urine investigation-manual and automatic; Measurement of serum/urine osmolality, electrolytes, glucose, urea – osmolality calculations; Measurement of acid-base parameters and blood gases (pH, pO2 , pCO2 ), impact of air bubbles on oxygen parameters; Protein electrophoresis on agarose gel, denzitometry, immunofixation; HPLC – sample preparation; Atomic absorptions spectrometry – determination of magnesium (phlame technik); copper determination (electrothermic atomization; graphite cuvette); Morphological investigation of cerebrospinal fluid – quantitative cytology (Fuchs-Rosenthal chamber), preparation of cytospine specimen; Biochemical investigation of cerebrospinal fluid, spectrophotometric curve; Internal and external quality assurane – Westgard rules; performance, assessment; Documents of good laboratory praxis and management quality according the ISO standards;
Literature
  • DASTYCH, Milan. Klinická biochemie : bakalářský obor Zdravotní laborant. 3. vyd. Brno: Masarykova univerzita, 2015, 254 pp. ISBN 978-80-210-7788-1. info
Teaching methods
independent solving of tasks from the analytical part of the course by using analyzers, instruments and laboratory equipment in a teaching laboratory and during the routine operation of a clinical laboratory; Writing protocols that contain measured values, their evaluation and conclusion; Continuous oversight of the assigned tasks, possible corrections and evaluation are performed by the lecturer;
Assessment methods
credit based on active attendance at practical laboratory work, written protocols and verification of attained knowledge and practical skills;
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2018, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2018/BLKB0321c