aVLIM051c Immunology - practice

Faculty of Medicine
autumn 2019
Extent and Intensity
0/2/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. MUDr. Tomáš Freiberger, Ph.D. (seminar tutor)
MUDr. Roman Hakl, Ph.D. (seminar tutor)
MUDr. Eva Hlaváčková, Ph.D. (seminar tutor)
doc. MUDr. Zita Chovancová, Ph.D. (seminar tutor)
prof. MUDr. Jiří Litzman, CSc. (seminar tutor)
Guaranteed by
prof. MUDr. Jiří Litzman, CSc.
Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: prof. MUDr. Jiří Litzman, CSc.
Supplier department: Department of Clinical Immunology and Allergology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
aVLIM051c/30: Fri 1. 11. 15:00–16:40 ÚKIA N04303, Fri 15. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/31: Fri 1. 11. 15:00–16:40 ÚKIA N04303, Fri 15. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/32: Fri 1. 11. 15:00–16:40 ÚKIA N04303, Fri 15. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/33: Fri 1. 11. 15:00–16:40 ÚKIA N04303, Fri 15. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/34: Fri 8. 11. 15:00–16:40 ÚKIA N04303, Fri 22. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/35: Fri 8. 11. 15:00–16:40 ÚKIA N04303, Fri 22. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/36: Fri 8. 11. 15:00–16:40 ÚKIA N04303, Fri 22. 11. 15:00–16:40 ÚKIA N04303
aVLIM051c/37: Fri 8. 11. 15:00–16:40 ÚKIA N04303, Fri 22. 11. 15:00–16:40 ÚKIA N04303
Prerequisites (in Czech)
( aVLBC0422p Biochemistry II - lect. || VSBC041p Biochemistry II -l ) && ( VSFY0422p Physiology II - lecture || aVLFY0422p Physiology II - lecture )
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
After finishing this course the student will understand the pathogenesis of immunopathological diseases and obtain the basic knowledge that he/she will aply in diagnosis and treatment of the most common disturbances of the immune system. In the laboratory part the students will be introduced to the techniques used in immunological laboratory, both serological (agglutination, precipitation, immunofluorescence, ELISA, RIA) and cellular (flow cytometry, lymphocyte proliferation tests, neutophil function assessment) to allow him to interpret the most common laboratory immunology methods in medicine. In the clinical part the mechanisms leading to immunopathological diseases such as clinical manifestation of those diseases will be discussed to allow the students to apply their knowledge in clinical medicine. The student will also discuss immunological problems associated with transplantation and malignancies.
Learning outcomes
The student will get acquainted with the basic methods of serological immunological diagnostics;
Th student will be acquainted with the basic methodologies for examining the number and function of the immune system cells;
The student will understand the importance, principle and clinical value of the diagnostic tests necessary for the diagnosis of allergic, autoimmune and immunodeficiency diseases;
The students will become acquainted with immunological laboratory aspects of transplantation and tumor growth.
Syllabus
  • Significance of the past history and of basic clinical and laboratory investigations for indication of immunological tests. Blood count. Serum proteins. Laboratory markers of inflammation. Blood collection. Diagnostic antisera.
  • Immunoglobulins and their biological functions. Monoclonal antibodies as diagnostic and therapeutic tools. Interaction between antigen and antibody in vitro. Primary and secondary immunological interactions.
  • Serological reactions - overview. Specificity and sensitivity. Titration of serum. Direct and indirect agglutination. Precipitation. Double immune diffusion. Turbidimetry, nephelometry. Immunofluorescence tests. Enzyme analysis (ELISA). RIA. Blotting techniques. Circulating and localised immune complexes. Complement system evaluation
  • Major histocompatibility complex: structure, function. HLA typing. Molecular genetic in immunology.
  • Innate or natural immunity: phagocytosis, NK cells, cytotoxicity, interferon system, complement system, acute phase reaction, inflammation. Complement system evaluation.
  • Cells of the immune system CD nomenclature of leukocyte antigens. Phenotyping of lymphocytes. Flow cytometry. Lymphocyte proliferation tests. Cytotoxicity. Evaluation of phagocytic cells.
  • Autoimmunity and disease. Immunological tolerance. Autoantibodies and their laboratory detection. Clinically significant organ specific and organ non-specific autoantibodies.
  • Immunoelectrophoresis, immunofixation. Immunophenotyping of leukemia. Clinical significance of paraproteinemia
Literature
    recommended literature
  • ABBAS, Abul K., Andrew H. LICHTMAN and Shiv PILLAI. Cellular and molecular immunology. 7th ed. Philadelphia: Elsevier/Saunders, 2012, x, 545. ISBN 9780808924258. info
Teaching methods
The course is given in a form of seminars and demonstration of laboratory methods.
Assessment methods
interview
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2022, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2019, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2019/aVLIM051c