LF:BLMB011c Mol.andCell.Biol.-pract. - Course Information
BLMB011c Molecular and Cellular Biology - practice
Faculty of Medicineautumn 2021
- Extent and Intensity
- 0/3/0. 2 credit(s). Type of Completion: z (credit).
- Teacher(s)
- prof. RNDr. Ondřej Slabý, Ph.D. (lecturer)
Mgr. Kateřina Cetkovská, Ph.D. (seminar tutor)
Ing. Lívia Eiselleová, Ph.D. (seminar tutor)
Mgr. Kateřina Francová, Ph.D. (seminar tutor)
Jana Růžičková (assistant) - Guaranteed by
- prof. RNDr. Ondřej Slabý, Ph.D.
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Kateřina Francová, Ph.D.
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine - Timetable
- Mon 13. 9. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 20. 9. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 27. 9. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 4. 10. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 11. 10. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 18. 10. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 25. 10. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 1. 11. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 8. 11. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 15. 11. 7:00–8:00 B07/205a, 8:00–9:00 B07/217, 8:00–9:00 B07/205a, 9:00–9:30 B07/205a, Mon 22. 11. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 29. 11. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 6. 12. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a, Mon 13. 12. 7:00–8:00 B07/205a, 8:00–9:00 B07/205a, 8:00–9:00 B07/217, 9:00–9:30 B07/205a
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Laboratory Technologist (programme LF, B-LABD)
- Laboratory Assistant (programme LF, B-SZ)
- Course objectives
- The main objectives of the course are:
- learning to operate the light microscope while observing prokaryotic and eukaryotic cells and various cell structures and processes
- understanding the methods of risk evaluation for monogenic hereditary diseases with autosomal inheritance in patients based on family anamneses - Learning outcomes
- After completing the course the student will be able to:
- operate the light microscope
- perform some of the basic cytochemical staining techniques in observation of bacterial and human cells
- explain the basic principles and use of electron microscopy
- describe the basic techniques and applications of tissue and cell cultures
- deduce the impact of selected DNA mutations on the primary structure of proteins
- explain the methodology of assembling and examining the karyotype and its use, and to evaluate basic numerical and structural aberrations in assigned human karyotypes
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunctions and chromosomal translocations
- evaluate the risk of monogenic hereditary diseases with autosomal inheritance in patients based on assigned family anamneses of the diseases - Syllabus
- Instructions. Non-cellular organisms and infectious agents
- Prokaryotic cells and their medical significance, basics of light microscopy
- Microscopic observation of eukaryotic cells
- Principles of electron microscopy, cellular ultrastructure
- Study of DNA damage
- Diagnostics of pathogenic bacteria
- Human karyotype and chromozomal aberrations
- DNA structure, gene expression and DNA replication
- In vitro culture of human cells
- Methods of cell cycle studying
- Mitosis observation under light microscope
- Biological significance of meiosis - gametogenesis
- Genetic disorders - autosomal and gonosomal inheritance
- Literature
- required literature
- Aktuální protokoly ke cvičením jsou dostupné v elektronické podobě na stránkách informačního systému MU (ISu): Studijní materiály předmětu BLMB011c
- Teaching methods
- laboratory practice, class discussion
- Assessment methods
- Practical classes are mandatory.
Requirements for course completion: full attendance in the practices (1 absence, excused or unexcused, is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; activity in the practices.
In case of student’s late arrival to the practice or poor activity at the practice, the student is required to write an essay in the length of 2 pages on a given topic. Same for the case of 2nd and any additional absence in practices (excused or unexcused). Find the instructions for essay writing in the Course-related instructions in the IS, bring the essay printed to the teacher who specified the topic and be ready to answer the teacher's questions regarding the topic.
In case of 3 absences, (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics from practices in a Credit test (20 questions, 4 options with only one of them being correct. At least 12 points out of 20 are needed to pass.)
In case of 4 or more unexcused absences, course-unit credit is not awarded. - Language of instruction
- Czech
- Follow-Up Courses
- Further comments (probably available only in Czech)
- The course is taught annually.
Information on the extent and intensity of the course: 45. - Listed among pre-requisites of other courses
- Teacher's information
- https://is.muni.cz/auth/el/1411/podzim2021/BLMB011c/index.qwarp
- Enrolment Statistics (autumn 2021, recent)
- Permalink: https://is.muni.cz/course/med/autumn2021/BLMB011c