VLBC0321s Biochemistry I - seminar

Faculty of Medicine
autumn 2022
Extent and Intensity
0/4/0. 7 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
prof. RNDr. Eva Táborská, CSc. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
doc. Mgr. Zdenka Fohlerová, Ph.D. (assistant)
Mgr. David Galuška (assistant)
Mgr. Patrícia Kittová (assistant)
doc. PharmDr. Jiří Kos, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
VLBC0321s/01: Mon 14:00–15:40 A16/215, Wed 10:00–11:40 A16/215, M. Králíková, J. Smutná
VLBC0321s/02: Mon 14:00–15:40 A16/215, Wed 10:00–11:40 A16/215, M. Králíková, J. Smutná
VLBC0321s/03: Mon 14:00–15:40 A16/213, Wed 14:00–15:40 A16/215, H. Paulová
VLBC0321s/04: Mon 14:00–15:40 A16/213, Wed 14:00–15:40 A16/215, H. Paulová
VLBC0321s/05: Mon 12:00–13:40 A16/215, Wed 12:00–13:40 A16/215, M. Králíková
VLBC0321s/06: Mon 12:00–13:40 A16/215, Wed 12:00–13:40 A16/215, M. Králíková
VLBC0321s/07: Tue 12:00–13:40 A16/213, Fri 12:00–13:40 A16/213, O. Peš, J. Smutná
VLBC0321s/08: Tue 12:00–13:40 A16/213, Fri 12:00–13:40 A16/213, O. Peš, J. Smutná
VLBC0321s/09: Mon 16:00–17:40 A16/215, Wed 14:00–15:40 A16/213, O. Peš
VLBC0321s/10: Mon 16:00–17:40 A16/215, Wed 14:00–15:40 A16/213, O. Peš
VLBC0321s/11: Mon 8:00–9:40 A16/213, Wed 8:00–9:40 A16/213, J. Slanina
VLBC0321s/12: Mon 8:00–9:40 A16/213, Wed 8:00–9:40 A16/213, J. Slanina
VLBC0321s/13: Tue 15:00–16:40 A16/213, Thu 15:00–16:40 A16/215, O. Peš, M. Tomandlová
VLBC0321s/14: Tue 15:00–16:40 A16/213, Thu 15:00–16:40 A16/215, O. Peš, M. Tomandlová
VLBC0321s/15: Mon 10:00–11:40 A16/213, Fri 10:00–11:40 A16/215, J. Dostál
VLBC0321s/16: Mon 10:00–11:40 A16/213, Fri 10:00–11:40 A16/215, J. Dostál
VLBC0321s/17: Mon 12:00–13:40 A16/213, Wed 16:00–17:40 A16/215, E. Táborská
VLBC0321s/18: Mon 12:00–13:40 A16/213, Wed 16:00–17:40 A16/215, E. Táborská
VLBC0321s/19: Mon 8:00–9:40 A16/215, Wed 8:00–9:40 A16/215, J. Gregorová
VLBC0321s/20: Mon 8:00–9:40 A16/215, Wed 8:00–9:40 A16/215, J. Gregorová
Prerequisites (in Czech)
VLBI0222p Biology II-lect. && VLBF011p Biophysics-lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cellular level. Understanding of these proceses is a base for comprihension of metabolism on the tissue and organ level. In the introductory lessons are summarized basic terms from chemistry needed for understanding of body structure a physico-chemical processes occuring in it ((chemical composition of the body, survay of biologically important elements,water, elektrolytes, non-elektrolytes, osmotic pressure, acid-base, redox and precipitation reactions), the following lectures are focused on biochemichal pathways in cells.
Learning outcomes
In the end of the course will students understand the meaning of basic chemical terms (pH, osmolality, electrolyte, buffer, etc.) and apply this knowledge when describing the properties of body fluids.
Describe the role of macro- and microbiogenic elements in the organism
Discusse the properties and function of enzymes
Describes basic catabolic and anabolic pathways of carbohydrate, lipid and protein metabolism, and their relationships.
Understand the principles of energy production, utilization and deposition at the cellular level.
Explain the function of cell membranes and the principle of compartmentalization at the cellular level and the transport processes on the membrane.
Describe protein synthesis, starting with the replication and transcription, translation and post-translational modifications. Understand the relationship between protein structure and function.
Explain the function of hemoglobin in oxygen transport and maintaining acid-base balance.
Discusses the principles of some diseases at the molecular level.
Syllabus
  • Chemical composition of human body.
  • Water, electrolytes, osmotic pressure, osmolality. pH, buffers. Structure of haemoglobin and its relationship to the function. Hb types in the blood of healthy subjects, HbCO and MetHb, abnormal Hb types. Enzymes - reaction rate, progress curve, the Michaelis plot and Km, enzyme inhibition. Coenzymes, their relationship to vitamins. Membrane structure and assembly. Transport across membranes. Metabolism of glucose: Glycolysis under anaerobic and aerobic conditions and the oxidation of pyruvate. Gluconeogenesis. Glycogenesis and glycogenolysis. Metabolism of proteins. Common features of amino acid conversion. The synthesis of urea. Nitrogen balance. Important reactions in amino acid catabolism. Biosynthesis and desaturation of fatty acids. The sources of essential fatty acids. Metabolism of triacylglycerols. Metabolism of phospholipids. Biosynthesis of eicosanoids. Peroxidation of lipids. The citric acid cycle. The respiratory chain and oxidative phosphorylation. Replication, transcription, proteosynthesis.
Literature
    required literature
  • Seminární texty dostupné v IS.
  • MURRAY, Robert K., David A. BENDER, Kathleen M. BOTHAM, Peter J. KENNELLY, Victor W. RODWELL and P. Anthony WEIL. Harperova ilustrovaná biochemie. Translated by Bohuslav Matouš. Páté české vydání, prv. Praha: Galén, 2012, xii, 730. ISBN 9788072629077. info
    recommended literature
  • LIEBERMAN, Michael and Alisa PEET. Marks' basic medical biochemistry : a clinical approach. Illustrated by Matthew Chansky. 5th edition. Philadelphia: Wolters Kluwer, 2018, xii, 1051. ISBN 9781496387721. info
  • VASUDEVAN, D. M., S. SREEKUMARI and Kannan VAIDYANATHAN. Úvod do všeobecnej a klinicky aplikovanej biochémie. Edited by Jozef Čársky. Prvé slovenské vydanie. Bratislava: Balneotherma s.r.o., 2015, 669 stran. ISBN 9788097015688. info
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Translated by Vladimír Benda - Martin Vejražka - Jiří Jonák. 1. české vydání. Praha: Grada, 2012, xiv, 498. ISBN 9788024729770. info
Teaching methods
Theoretical preparation and discussion on individual topics.
Assessment methods
Course-unit credit. Conditions for giving the course-unit credit (all conditions should be met):
  • Full attendance in lessons. All absences should be justified and made up.
  • At least 70% success rate from partial revision tests or 50% success rate in credit test, the resit credit test can be taken only once.
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    Information on the extent and intensity of the course: 60.
    Listed among pre-requisites of other courses
    The course is also listed under the following terms Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2023, autumn 2024.
    • Enrolment Statistics (autumn 2022, recent)
    • Permalink: https://is.muni.cz/course/med/autumn2022/VLBC0321s