aVLMB031 Modern Imaging and Analytical Methods in Cellular Biology and Immunology

Faculty of Medicine
autumn 2022
Extent and Intensity
0/0.7/.3. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
Mgr. Kamila Bendíčková, Ph.D. (lecturer)
Mgr. Jan Frič, Ph.D., MHA (lecturer)
Mgr. Marcela Hortová Kohoutková, Ph.D. (lecturer)
Mgr. Petra Lázničková, Ph.D. (lecturer)
Mgr. Ivana Andrejčinová (seminar tutor)
Mgr. Veronika Bosáková, Ph.D. (seminar tutor)
Sofia Morazzo (seminar tutor)
Mgr. Ondřej Vymazal (seminar tutor)
Guaranteed by
Mgr. Jan Frič, Ph.D., MHA
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Mgr. Jan Frič, Ph.D., MHA
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine
Timetable
Fri 14. 10. 9:00–15:00 Virtuální místnost LF/Virtual room LF, Fri 21. 10. 9:00–16:00 A 1.08
Prerequisites
aVLBI0222p Medical Biology II - lect. || aZLBI0222p Biology II-lect.
Students need to have cell biology knowledge at the level of 1st year of university, proven with an exam (aVLBI0222p, aZLBI0222p or Bi1700).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 40 student(s).
Current registration and enrolment status: enrolled: 9/40, only registered: 0/40
fields of study / plans the course is directly associated with
Course objectives
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); confocal microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Learning outcomes
After completion of the course student: gets an overview of currently used methods of molecular and cell imaging and analysis; and will see some of their applications in practice. Students will see following laboratories and devices in operation: imaging of biomolecules in cells and tissues (cell-imaging); confocal microscopy; flow-cytometry. Students will understand the principle and possibilities of application of these methods; will be more familiar with the modern study of biological material and their use in biomedical research and modern medicine, and will be capable of basic interpretation of results obtained from these methods.
Syllabus
  • 14.10.2022: Online lectures 9:00-15:00 (MS Teams)
  • Lessons will be recorded and uploaded into study materials:
  • - Principles of confocal microscopy and applications
  • - Principles of flow cytometry and cell phenotype analysis using flow cytometry
  • - Cell separation methods (FACS sorting)
  • - Advanced flow cytometry methods, Spectral cytometry, Mass cytometry
  • - Advanced microscopy techniques - electron and multiphoton microscopy
  • - Other molecular biology methods for proteins and RNA analysis
  • 21.10.2022: In-person meeting (Biology park Bohunice near Campus)
  • Morning session 9:00-11.15
  • - Imaging and analytical methods in clinical research
  • Afternoon session from 12:00
  • - Lab tour with methods demonstration
  • Final test:
  • - 2 open questions from each theoretical online presentation
  • Contact person: ovymazal@mail.muni.cz
Literature
  • Relevant textbooks chapters and links to internet sources that will help to understand the basic principles of the methods will be provided during the course.
Teaching methods
a seminar, lab demonstration
Assessment methods
Course completion requires full attendance and passing a final written test (10 questions about the basic principles of demonstrated methods).
Attendance at an online seminar is highly recommended; in-person seminars require full attendance.
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 15.
Teacher's information
https://is.muni.cz/auth/el/1411/podzim2016/VLMB011/index.qwarp
The course is also listed under the following terms Autumn 2017, autumn 2018, autumn 2020, autumn 2021, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2022/aVLMB031