aZLBI0121c Biology I - practice

Faculty of Medicine
autumn 2023
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Ondřej Slabý, Ph.D. (lecturer)
prof. MUDr. Iva Slaninová, Ph.D. (lecturer)
Ing. Lívia Eiselleová, Ph.D. (lecturer)
Mgr. Kateřina Cetkovská, Ph.D. (lecturer)
Mgr. Kateřina Francová, Ph.D. (lecturer)
Mgr. Kateřina Straková, Ph.D. (lecturer)
Mgr. Andrej Bešše, Dr. sc. nat. (seminar tutor)
Mgr. Lenka Bešše, Ph.D. (seminar tutor)
Pooja Dudeja (seminar tutor)
Monica Medappa (seminar tutor)
Mgr. Petra Pospíšilová, Ph.D. (seminar tutor)
Ing. Mgr. Michaela Ručková (seminar tutor)
Vlad-Constantin Ursachi (seminar tutor)
Ing. Natália Vadovičová (seminar tutor)
Jana Růžičková (assistant)
Guaranteed by
prof. MUDr. Iva Slaninová, Ph.D.
Department of Biology – Theoretical Departments – Faculty of Medicine
Contact Person: Ing. Lívia Eiselleová, Ph.D.
Supplier department: Department of Biology – Theoretical Departments – Faculty of Medicine
Timetable
Thu 13:00–13:30 B07/214, Thu 13:30–14:30 B07/217, Thu 13:30–14:30 B07/214, Thu 14:30–14:40 B07/214
Prerequisites
Knowledge of high-school biology on the level of Overview of High School Biology (https://is.muni.cz/auth/do/med/el/overview/overview_ENG.qwarp) or similar is required.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The main objectives of the course are:
- learning to operate the light microscope while observing prokaryotic and eukaryotic cells and various cell structures and processes
- understanding the methodology of assembling and examining the karyotype (evaluation of basic numerical and structural aberrations)
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunction and chromosomal translocations
- use some of the basic techniques of molecular biology to monitor the cell cycle and cell death
- describe the stages of cell division
Learning outcomes
After completing the course, the student will be able to:
- operate the light microscope
- perform some of the basic cytochemical staining techniques in observation of bacterial and human cells
- explain the basic principles and use of electron microscopy
- describe the basic techniques and applications of tissue and cell cultures
- deduce the impact of selected DNA mutations on the primary structure of proteins
- explain the methodology of assembling and examining the karyotype and its use, and evaluate basic numerical and structural aberrations in assigned human karyotypes
- evaluate the risk of numerical and structural aberrations resulting from meiotic non-disjunction and chromosomal translocations
Syllabus
  • Week 1: Instructions. Non-cellular organisms and infectious agents
  • Week 2: Prokaryotic cells, basics of light microscopy
  • Week 3: Microscopic observation of eukaryotic cells
  • Week 4: In vitro culture of human cells
  • Week 5: Principles of electron microscopy, cellular ultrastructure
  • Week 6: Control test 1 (knowledge of the weeks 1 to 5 - from lectures and practices) + pipetting workshop
  • Week 7: DNA structure, DNA replication, gene expression
  • Week 8: Human karyotype and chromosomal aberrations
  • Week 9: Study of DNA damage
  • Week 10: Methods for studying cell cycle
  • Week 11: Mitosis and meiosis observation under light microscope
  • Week 12: Methods for studying cell death
  • Week 13: Control test 2 (knowledge of the weeks 7 to 12 - from lectures and practices) + microscopy exam
  • Week 14: practices do not take place/re-sit of control tests
Literature
    required literature
  • Actual protocols for practices are provided in electronic form in the Information System of the Masaryk University (IS): Study Materials of the course aZLBI0121c
Teaching methods
laboratory practice, class discussion
Assessment methods
Practical classes are mandatory.
Requirements for course completion: full attendance in the practices (1 unexcused absence is allowed); all protocols completed (also for missed lessons) and checked and signed by appropriate teachers; successfully passed 2 written control tests during the semester (knowledge from lectures and practices, 20 questions in each test, 1 answer correct to be chosen from 4 possibilities, student needs to reach at least 24 points out of 40 from both of the tests). In case you do not pass control tests (you have less than 24 points), you write a re-sit of control test (knowledge from lectures and practices of entire semester, 20 questions, 1 answer correct to be chosen from 4 possibilities, student needs to reach at least 12 points). If you do not pass the re-sit of control test, you have to write the final test (Credit test) based on knowledge of the entire semester lectures and practices. Credit test comprises 20 questions: 10 test questions (multiple answers can be correct, negative marking is obtained for incorrect answers) + 10 given terms for a written explanation. At least 10 points out of 20 are needed to pass.
In case of a student’s late arrival to the practice or poor activity at the practice, the student is required to write an essay in the length of 2 pages on a given topic. Same for the case of two unexcused absences in practices. Find the instructions for essay writing in the Instructions for the course in Interactive syllabus in the IS. Bring the essay printed to the teacher who specified the topic (after making an appointment in consulting hours), and be ready to answer the teacher's questions regarding the topic.
In case of 3 absences (excused or unexcused), (or more than 3 absences all excused by the Office for Studies within 5 days from the beginning of the absence and introduced to the Information System), it is addressed individually and course-unit credit is awarded only after the proof of the student's knowledge of the entire semester topics (Credit test, see above).
In the case of 4 or more unexcused absences, the course-unit credit is not awarded.
Language of instruction
English
Follow-Up Courses
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
Listed among pre-requisites of other courses
Teacher's information
https://is.muni.cz/auth/el/med/podzim2023/aZLBI0121c/index.qwarp
Interactive syllabus of the course: https://is.muni.cz/auth/el/med/podzim2023/aZLBI0121c/index.qwarp

What to bring to each practice: printed protocol for the respective practice, your own writing materials. Download the protocol for each practice from the Study materials or Interactive syllabus in the IS, and study it in advance. Lab shoes/slippers are required; lab coats and disposable gloves are not required.

Substitution of practices: In exceptional cases (illness, appointment at the doctor or foreign policy, once or two times per semester as maximum) student can arrange a substitution with another group. To arrange a substitution, write an e-mail to the contact person at least 24 hours before your planned substitution and specify the group (date and time) you would like to attend instead (see Interactive syllabus - Instructions for the course - Timetable). Substitution is only possible on the same teaching week. When the capacity of the group you intended to substitute with is fully used, the substitution does not need to be approved. Repeated substitution with other groups is not allowed.

Complete Biology instructions to be found in Interactive syllabus of the course.

The course is also listed under the following terms Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2022, autumn 2024.
  • Enrolment Statistics (autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2023/aZLBI0121c