BLIT0222p Instrument Technology II - lecture

Faculty of Medicine
Spring 2013
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
RNDr. Miroslava Beňovská, Ph.D. (lecturer)
RNDr. Petr Breinek (lecturer)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Mon 13:20–15:00 B09/324
Prerequisites (in Czech)
BLIT0121c Instrument Technology I - pr. && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The goal of the theoretical preparation part is to provide a theoretical base of physical principles used in analytical and laboratory technology. To familiarize students in detail with the application of these physical principles in specific instruments including existing modifications in contemporary medical laboratory equipment. To observe variations and modifications of specific examples and determine their purpose, advantages and critical sections in seminars, demonstrations and practical exercises.; A student will be able to do the following upon completing the course: Have an overview of new instrumental technologies; Understand the technical parameters of laboratory instruments and be able to compare them with one another; Judge the advantages, disadvantages and limitations of individual instrumental and analytical systems; Apply acquired theoretical knowledge to practical work in a laboratory; Follow standard technical operational procedures;
Syllabus
  • Basic physical principals used in instrumental technology; Optical methods (spectrophotometry, turbidimetry, fluorimetry, nephelometry, denzitometry, refractometry, reflectance photometry, phlame emission photometry, atomic absorption spectrophotometry; Electrochemical methods (potentiometry, amperometry, polarography, coulometry, conductometry, electrophoresis); Ion-selective electrodes (ISE); Kryoscopy, ebullioscopy; Microscopy; Radioactive isotopes; Centrifugation; Basic physical principals used in instrumental technology – Demonstration; Spectrophotometer-design (sources of lightradiation, monochromatores, interference filtrs, cuvettes, detectors; Phlame emission photometer -design, sampling of analytical sample, internal standard; pH meter; Polarograph; Coulometer; Conductometer; Denzitometer; Application of basic physical principals in laboratory instrumentation; Spectrophotometer UV/VIS, fluorimeter, nephelometer, denzitometer, refractometer, reflectance photometer, phlame emission photometer, atomic absorption spectrophotometer; pH meter, polarograph, coulometer, conductometer; electrophoretic device (agarose, polyakrylamid, isoelectric focusation); Thin layer chromatography device, liquid chromatograph-HPLC, gas chromatograph-GC; Osmometer; ISE (Na, K, Cl, Ca, Mg); Clarck electrode (pO2), Severinghaus electrode (pCO2 ), enzymatic electrodes; Centrifuges; Termostats, incubation bathes; Microwave mineralization device; Devices for RIA; Luminometer;
Literature
  • DASTYCH, Milan. Instrumentální technika : obor zdravotní laborant. 1. vyd. Brno: Masarykova univerzita, 2007, 131 s. ISBN 9788021042261. info
Teaching methods
a lecture with the use of audiovisual teaching aids (pp-presentations, commented videos) including interactive input;
Assessment methods
two written tests, a final oral examination with questions from the general and specialized portions of the course;
Language of instruction
Czech
Further Comments
Study Materials
The course is also listed under the following terms Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2013, recent)
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