LF:BLTS0411p Immunohaematology - lec. - Course Information

BLTS0411p Immunohaematology and Blood Banking - lecture

Extent and Intensity

2/0/0. 3 credit(s). Type of Completion: zk (examination).

Teacher(s)

MUDr. Eva Tesařová (lecturer), doc. MUDr. Milan Dastych, CSc., MBA (deputy)
Bc. Dana Peňázová (assistant)

Guaranteed by

doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine

Timetable

Mon 7:30–9:10 N01231

Prerequisites (in Czech)

BLKH0311c Clinical Haematology - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

• Laboratory Assistant (programme LF, B-SZ)

Course objectives

Course main objectives The aim of theoretical part of education in immunohaematology is given to understand separate laboratory procedures principles which are currently used in immunohaematology and acquirement of knowledge in relation to its application in laboratory praxis. The accent is putting on ability of separate laboratory results interpretation and/or group laboratory results interpretation to clinical patient hood. Professional theoretical training in HLA covers laboratory testing HLA complex and laboratory results interpretation in relation to solid organs transplant strategy and in relation to strategy of bone marrow donors registry as far as laboratory results interpretation in relation to immunized patients. Theoretical part of education of transfusion service covers background and principles of good manufacturing praxis in preparation, use and quality assurance of transfusion products (allogeneic, autologous) and plasma for fractionation, including quality management and process control, personnel and organisation, equipment and materials, documentation, donor selection, processing, storage and distribution, quality monitoring, quality control, contract management, deviations, complaints, corrective and preventive actions, self inspection, audits and improvement, data processing systems, record keeping, statistical process control, background of safe haemotherapy, principles of haemovigilance system, including types of adverse reactions description and its reporting. After course completing programme the student will be able: - transfusion service legislation orientate, - respect principles of good laboratory praxis and good manufacturing praxis, - know principles of haemovigilace and haemotherapy, - qualify accuracy of indication and interpretation of immunohaematology laboratory results, - recognize cases for following testing, - analytic methods orientate.

Syllabus

• Laboratory theory Introduction, definition of immunohaematology, HLA, transfusion service ; Laboratory diagnostics in immunohaematology ; Red blood cell antigens and antibodies, background of blood groups inheritance ; AB0 blood group system, discovery and development of blood group knowledge, state of art, possibilities of detection, importance, ; Lewis blood group system, secretor status ; Rh blood group system ; Others blood group systems, low-prevalence, high-prevalence antigens ; Clinical significance of blood group antibodies, medical aspects, laboratory procedure, screening and identification, selection, crossmatching ; Leucocytes and platelets antigens, production of antibodies and its medical aspects, possibilities of laboratory detection ; Laboratory diagnostics in HLA ; HLA system, medical aspects of HLA antigens, HLA antibodies ; HLA system link to solid organs transplant strategy and bone marrow donors registry; Background of good laboratory praxis ; Internal and external quality process control; Preanalytic, analytic and postanalytic fasis of laboratory procedure ; Good manufacturing praxis in preparation, use and quality assurance of transfusion products (allogeneic, autogenous) and plasma for fractionation,; Transfusion preparations, blood derivates, definition, indication for clinical use ; Plasma for fractionation, definition ; Blood collection, testing ; Autologous transfusion preparations; Legislature on transfusion service, audits of state authority; Organisation of transfusion service in Czech republic ; Data processing systems, record keeping, background of on-line data transfers ; Statistical process control ; Clinical theory ; Safe haemotherapy, ordering and administration, transfusion equipment, uses and contraindications, expected response and potential adverse effects, alternatives to allogeneic transfusion, registry of rare blood group donors; Transfusion management of infants and children, haemolytic disease of foetus and newborn, AB0 and Rh incompatibility, others blood group incompatibility special requirements for intrauterine and neonatal transfusions ; Autoimmune haemolytic anaemia, definition, medical aspects, selection, laboratory testing, safe transfusion, options of haemotherapy ; Acute and delayed transfusion reactions, laboratory findings, clinical evaluation at the bedside; Principles and usage of specific laboratory testing in immunohaematology, absorbing tests, elution techniques, neutralising tests; Quality assurance in immunohaematology; Trouble-shooting and problem-solving in laboratory ; Transplant of bone marrow and haemotherapy blood group conversion ; Infectious complications of transfusion ; Informed consent for transfusion, lookback and recipient notification and product recall and withdrawls ; Haemovigilance system, transfusion committee, process improvement;

Literature

• HRABČÁKOVÁ, Viera, Petr ČOUPEK, Marie NENIČKOVÁ and Eva TESAŘOVÁ. Hladiny cytokinů a kostimulačních molekul (interleukinů IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6) v plazmě zdravých dárců krve. (Plasma levels of cytokines and co-stimulatory molecules (interleukins IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, TNF-alpha, TGF-beta1, sCD23, sCD40L and receptor for IL-6) in healthy blood donors.). Transfuze a hematologie dnes. Praha: Česká lékařská společnost JEP, 2006, vol. 12, No 3, p. 166-172. ISSN 1213-5763. info
• KLABUSAY, Martin, Viera HRABČÁKOVÁ, Petr ČOUPEK, Marie NENIČKOVÁ and Eva TESAŘOVÁ. Simultaneous analysis of cytokines and co-stimulatory molecules concentrations by ELISA technique and of probabilities of measurable concentrations of interleukins occurring in plasma of healthy blood donors. New York: Hindawi Publishing Corporation, 2006. ISSN 0962-9351. info
• HRABČÁKOVÁ, Viera, Petr ČOUPEK, Marie NENIČKOVÁ and Eva TESAŘOVÁ. Hladiny cytokinů a kostimulačních molekul (interleukinů-2, 4, 5, 6, 8, 10, 13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6) v plazmě zdravých dárců krve. (Plasma levels of cytokines and co-stimulatory molecules (interleukin-2, 4, 5, 6, 8, 10, 13, TNF-alpha, TGF-beta1, sCD23, sCD40L and receptor for IL-6) in healthy blood donors.). In XIV. Slovensko-český hematologický a transfuziologický zjazd s medzinárodnou účasťou, 29.9.-2.10.2005, Štrbské Pleso. 2005. ISBN 80-968685-9-4. info
• HRABČÁKOVÁ, Viera, Marie NENIČKOVÁ, Eva TESAŘOVÁ and Martin KLABUSAY. Hladiny interleukinů-2, 4, 5, 6, 8, 10, 13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6 v plazmě zdravých dárců krve. (Plasma levels of interleukin-2, 4, 5, 6, 8, 10, 13, TNF-alpha, TGF-beta1, sCD23, sCD40L and sIL-6 receptor in healthy blood donors.). In XIX. Olomoucké hematologické dny s mezinárodní účastí. 2005. ISBN 80-7346-051-3. info
• HRABČÁKOVÁ, Viera, Marie NENIČKOVÁ, Eva TESAŘOVÁ and Martin KLABUSAY. Stanovení interleukinů-2, 4, 5, 6, 8, 10, 13, TNF-alfa, TGF-beta1, sCD23, sCD40L a receptoru pro IL-6 v plazmě zdravých dárců krve. (Detection of plasma levels of interleukin-2, 4, 5, 6, 8, 10, 13, TNF-alpha, TGF-beta1, sCD23, sCD40L and receptoru pro IL-6 in healthy blood donors.). In Analytická cytometrie III, konference se zahraniční účastí, Červenohorské sedlo, 21.-25.6.2005. 2005. ISBN 80-239-5155-6. info

Teaching methods

- audiovisual lectures (pp-presentations), - excursions to laboratory and manufacturing spaces - technical handbook, - professional discussion.

Assessment methods

Three tests with questions, which cover all field of immunohaematology and transfusion service.

Language of instruction

Czech

• Enrolment Statistics (Spring 2013, recent)
  
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