BLTM0211c Techniques of Molecular Biology and Genetics - practice

Faculty of Medicine
Spring 2014
Extent and Intensity
0/3/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Michal Jurajda, Ph.D. (seminar tutor)
prof. RNDr. Michal Masařík, Ph.D. (seminar tutor)
prof. MUDr. Anna Vašků, CSc. (lecturer)
Dipl. Ing. Martin Piskáček, Dr. rer. nat. (seminar tutor)
doc. RNDr. Sabina Ševčíková, Ph.D. (seminar tutor)
Mgr. Bc. Tomáš Pruša (assistant)
Guaranteed by
MUDr. Michal Jurajda, Ph.D.
Department of Pathophysiology – Theoretical Departments – Faculty of Medicine
Contact Person: Věra Ježková
Supplier department: Department of Pathophysiology – Theoretical Departments – Faculty of Medicine
Timetable
Fri 13:30–16:00 A18/108
Prerequisites (in Czech)
BLMB011c Mol.andCell.Biol.-pract. && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
At the end of this course, students should be able to understand principles of basic techniques of molecular biology and human genetics. They should understand stucture of human genome, principles of genome analyses and principles of heritability. They should be able to employ basic mathods of molecular biology and genetics.
Syllabus
  • Introduction, basic statistics in analysis of experimental results.
  • Internet resources for molecular biology, databases and tools (Web of Science, Pubmed..).
  • Computer assisted image analysis. Light microscopy, basics of photographic techniques.
  • Workflow of handling with samples of biologic material.
  • Enzymes as a markers of diseases, electrophoretic analysis of proteins.
  • Polyacrylamid gel electrophoresis with SDS (SDS-PAGE). Principles of the method, densitometric analysis of electrophoregrams.
  • Western blotting and immunochemistry.
  • Nucleid acids isolation, principles of polymerase chain reaction (PCR).
  • Detection of single nucleotide polymorphisms using PCR a agarose gel electrophoresis.
  • Computer assisted analysis of agarose gel electrophoresis results.
  • Cell cultures.
  • DNA sequencing, DNA quantitation using real-time PCR.
  • Pharmacogenomics and gene therapy.
Literature
  • ALBERTS, Bruce. Základy buněčné biologie :úvod do molekulární biologie buňky. 1. vyd. Ústí nad Labem: Espero, 1999, xxvi, 630. ISBN 80-902906-0-4. info
Teaching methods
practical training
Assessment methods
Full attendance with only one absence and written experimental records are necessary for giving credits.
Language of instruction
Czech
The course is also listed under the following terms Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2014, recent)
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