MOEB1041c Experimental biophotonics - practice

Faculty of Medicine
Spring 2015
Extent and Intensity
1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. RNDr. Radim Chmelík, Ph.D. (seminar tutor)
Anna Petruželková (assistant)
Guaranteed by
prof. RNDr. Radim Chmelík, Ph.D.
Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Anna Petruželková
Supplier department: Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Timetable
Thu 11:00–11:50 VUT-A4/501
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is intended to provide an overview of modern experimental techniques used to determine structure, function and dynamics of macro and micro biological samples by means of electromagnetic radiation. Attention will be focused on non-invasive methods used for detection, diagnosis and treatment of diseases, including methods used in ophthalmology and optometry. Prerequisites Students are expected to have theoretical knowledge as from "Applied optics" course. Acquired knowledge and skills Deeper theoretical and chiefly practical knowledge of some modern techniques that use electromagnetic radiation to study structure, function and dynamics of biological samples and methods used to detect, diagnose and treat diseases.
Syllabus
  • 1. Brief summary (reminding) of fundamentals in: Electromagnetic radiation. Structure of matter. Structure of atom. Structure of molecule. Energy levels of electrons. Absorption and emission of radiation. Spectra of atoms and molecules. Light. X-ray and gamma rays. Laboratory: Diffraction on a grating. Measurement of spectra of different light sources using a spectrometer. Measurement of coherence length using an interferometer. 2. Brief summary (reminding) of fundamentals in: Propagation of light and its interactions. Electromagnetic waves, photons. Description and properties (interference, diffraction, energy, momentum). Filters of light (interference, neutral, polarizing), filters in ophthalmology. UV filter and glasses. 3. Sources of radiation, lasers (reminding of the priciple). Laser types, use in ophthalmology. Properties of the laser beam. Detectors of radiation (classical and digital camera - principle and applications in medicine). Luxmeter. Detection of UV and IR radiation. Remote TV controls. Laboratory: Diffraction of a laser beam on 2D objects (apertures in opaque screen), calculation of parameters of the apertures from the diffraction image. 4. Interactions of radiation with a matter and how it affects tissues. Absorption, transmission, emission + spectroscopy. 5. Applications in diagnosis and therapeutic methods: tomography, irradiation, "light acupuncture" Laboratory: LIBS and tomograph. 6. Optical micromanipulations. Optical tweezers and scalpel. 7. Other practical applications of lasers in medicine (ophthalmology): LASIK, femto-LASIK, surgery, stomatology (dental laser, photoactivation), dermatology, cosmetics, cytometry, microdissection. Laboratory: Application of laser scalpel - microdissection technique. 8. Brief summary of fundamentals in optical microscopy: bright and dark field, Zernike phase contrast, polarization microscopy, differential interference contrast (DIC), 3D visualization methods. Laboratory: Practical demonstrations of the common methods enhancing contrast in an optical microscope. 9. Brief summary of fundamentals in confocal microscopy and OCT/OCM. Holographic microscopy. 3D image formation. Endoscopy. 10. Fluorescence microscopy and its modifications (fluorescence resonance energy transfer - FRET, total internal reflection fluorescence - TIRF), raman spectroscopy, multiphoto microscopy, second harmonic generation - SHG, stimulated emission depletion - STED, structured illumination microscopy - SIM, light-sheet microscopy. Laboratory: Confocal and fluorescence microscopy in biology. Application of holographic microscopy in study of alive mammalian cells. 11. Photodynamic therapy, optical biosensors. 12. Applications of micro and nano opto-technologies in medicine (retinal implants), nanosurgery.
Literature
  • J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995.
  • P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003.
Teaching methods
practice
Assessment methods
fulfilling requirements
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is also listed under the following terms Spring 2014, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2015, recent)
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