BLKBC0211p Biochemistry - lecture

Faculty of Medicine
Spring 2017
Extent and Intensity
0/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Jiří Dostál, CSc. (lecturer)
RNDr. Hana Paulová, CSc. (lecturer)
Mgr. Jiří Slanina, Ph.D. (lecturer)
prof. RNDr. Eva Táborská, CSc. (lecturer)
doc. RNDr. Josef Tomandl, Ph.D. (lecturer)
MUDr. Michaela Králíková, Ph.D. (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: Lenka Nerudová
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Prerequisites (in Czech)
BLKLC011s Med.Chem. seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to obtain knowledge on essential metabolic processes on the cell level. The graduate is acquainted with properties and function of enzymes, is oriented in catabolic and anabolic pathways of metabolism of saccharides, lipids and proteins and their consequences.Understands the principles of cell bioenegetics.Become familiar with the funcion of cell membranes, principle of cell compartmentation and membrane transport.Has knowledge on proteosynthesis including processes replication,transcription, translation and posttranslantion modifications.Understands the relation between the structure and function of proteins. Is acquinted with function of hemoglobine in oxygen transport.Molecular bases of some diseases are introduced. The course provides the essential knowledge for future understanding of metabolism on organe and inter-organe level and its disturbances.
Syllabus
  • Membrane structure, the assembly and recycling of membranes. Specialized structures of plasma membrane – lipid rafts, caveols, tight junctions. membrane transport. Enzymes. Characteristic features of biocatalysis, enzyme structure and function, nomenclature and classification of enzymes. Enzyme cofactors, review of structures and functions. Mechanisms of enzyme action. Kinetics of enzyme catalyzed reactions. Assays of enzyme activity, the conditions used. Factors affecting catalytic activity of enzymes, types of enzyme inhibition. Metabolism: basic concepts and design. Biological oxidations, generation of high-energy compounds. Saccharide metabolism: the glycolytic pathway and aerobic decarboxylation of pyruvate. Gluconeogenesis. Glycogen biosynthesis and breakdown. The pentose phosphate pathway. The glucuronate pathway. Interconversions of monosaccharides and of their derivatives. Protein and amino acid metabolism. The common reactions in amino acid degradation. The ureosynthetic cycle. Metabolic breakdown of individual amino acids. Biosynthesis and breakdown of fatty acids, ketogenesis. Synthesis of triacylglycerols. Metabolism of phospholipids and glycolipids. Synthesis of eicosanoids. Biosynthesis and transformations of cholesterol, biosynthesis of bile acids. Interrelationships among the major pathways involved in energy metabolism. The citric acid cycle. Synthesis of haem. Mitochondria. Oxidative phosphorylation - mitochondrial electron transport chain, synthesis of ATP. Structure of haemoglobin, structure-function relationships (the oxygen saturation curve, inducement of haemoglobin saturation and oxygen transport. Bohr effect. Normal haemoglobin types in blood, haemoglobin concentration. Other forms (glycohaemoglobin, methaemoglobin, carboxyhaemoglobin) and abnormal haemoglobins. Biosynthesis and catabolism of purine and pyrimidine nucleotides. Chromatin, DNA replication. DNA transcription. Regulation of gene expression. Protein synthesis and post-translational processing.
Literature
    required literature
  • KOOLMAN, Jan and Klaus-Heinrich RÖHM. Barevný atlas biochemie. Grada. Praha, 2012, 498 pp. ISBN 978-80-247-2977-0. info
    recommended literature
  • LEDVINA, Miroslav, Alena STOKLASOVÁ and Jaroslav CERMAN. Biochemie pro studující medicíny. Vyd. 1. Praha: Karolinum, 2004, s. 281-562. ISBN 8024608510. info
Teaching methods
Teaching form are lectures. Supplementary subjects are seminars BLBC021s and practicals BLBC021c.
Assessment methods
Course is completed by oral examination. Student must have course-unit credits of both paralel courses in the day of exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on completion of the course: Přihlášení ke zkoušce je podmíněno složením zkoušky z Lékařské chemie
The course is taught annually.
The course is taught: every week.
General note: Všechny formy kontaktní výuky předmětu vyjma přednášek jsou povinné.
Information on the extent and intensity of the course: 10 hodin/semestr.
Information on course enrolment limitations: Přihlášení ke zkoušce je podmíněno vykonáním zkoušky z Lékařské chemie.
The course is also listed under the following terms Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2018, spring 2019, spring 2020.
  • Enrolment Statistics (Spring 2017, recent)
  • Permalink: https://is.muni.cz/course/med/spring2017/BLKBC0211p