VLLM0421c Medical Microbiology I - practice

Faculty of Medicine
spring 2019
Extent and Intensity
0/3/0. 4 credit(s). Type of Completion: z (credit).
Teacher(s)
MUDr. Ondřej Zahradníček (lecturer)
MUDr. Lenka Černohorská, Ph.D. (seminar tutor)
Mgr. Milada Dvořáčková, Ph.D., MBA (seminar tutor)
Mgr. Monika Dvořáková Heroldová, Ph.D. (seminar tutor)
Ing. Veronika Holá, Ph.D. (seminar tutor)
Mgr. Ing. Veronika Korecká (seminar tutor)
prof. MUDr. Filip Růžička, Ph.D. (seminar tutor)
MUDr. Alena Siváková (seminar tutor)
Mgr. Lukáš Vacek, Ph.D. (seminar tutor)
Guaranteed by
prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable of Seminar Groups
VLLM0421c/01_02: Mon 16:00–18:30 MiÚ N02112, Mon 16:00–18:30 MiÚ N02112, M. Dvořáková Heroldová
VLLM0421c/03_04: Tue 13:00–15:30 MiÚ N02112, Tue 13:00–15:30 MiÚ N02112, V. Korecká
VLLM0421c/05_06: Wed 18:00–20:30 MiÚ N02112, Wed 18:00–20:30 MiÚ N02112, M. Dvořáčková
VLLM0421c/07_08: Mon 13:00–15:30 MiÚ N02112, Mon 13:00–15:30 MiÚ N02112, M. Dvořáková Heroldová
VLLM0421c/09_10: Wed 10:10–12:40 MiÚ N02112, Wed 10:10–12:40 MiÚ N02112, L. Černohorská
VLLM0421c/11_12: Wed 13:00–15:30 MiÚ N02112, Wed 13:00–15:30 MiÚ N02112, O. Zahradníček
VLLM0421c/13_16: Mon 7:30–10:00 MiÚ N02112, Mon 7:30–10:00 MiÚ N02112, A. Siváková, O. Zahradníček
VLLM0421c/14_15: Thu 18:00–20:30 MiÚ N02112, Thu 18:00–20:30 MiÚ N02112, O. Zahradníček
VLLM0421c/41: Fri 10:10–12:40 MiÚ N02112, V. Korecká
VLLM0421c/42: Mon 10:10–12:40 MiÚ N02112, L. Vacek
Prerequisites
( VSBI0222p Biology II-lect. || VLBI0222p Biology II-lect. ) && ( VSBF011p Biophysics || VLBF011p Biophysics-lect. )
Students are supposed to have considerable knowledge of anathomy, histology, biology, biochemistry and physiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to teach students to define the basic concepts of medical diagnostic virology, mycology and parasitology
Learning outcomes
At the end of this course, students should be able:
to prepare wet mount, Gram stained preparation, to observe the results in a microscope, to list other staining methods
to list culture media, their use, differences between them, to reinoculate a strain/specimen to a culture medium
to identify bacteria and yeasts by means of various methods of biochemical identification and other identification methods
to describe antibiotic susceptibility/resistance testing methods and to read their results
to describe animal experiment (only basically)
to read results of nucleic acid detection in microbiology (only basically)
to describe methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; to read the results of these methods including titers, titer dynamics, detection of IgM/IgA/IgG; to interprete the results; to describe precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting
Besides that, students will be able to describe the basic of medical virology
Syllabus
  • J01 Safety in laboratory, technique of work with loop, wet mount. Gram staining, demonstration of more staining methods. Demostration of pictures of various organisms and structures in Gram staining.
  • J02 Bacterial culture
  • J03 Physiology of bacteria, idenctification according to biochemical activity and virulence factor determination
  • J04 Molecular methods in microbiology
  • J05 Microbes and outer influences, decontamination methods
  • J06 Testing of bacterial susceptibility to antimicrobial drugs, detection of resistance factors
  • J07 Biofilm infections
  • J08 Introduction to serology, agglutination and precipitation, dilution and measuring titres
  • J09 Following dynamics of titres, complement fixing test, neutralisation
  • J10 Reactions with labelled components (IMF, RIA, ELISA) including immunoblotting
  • J11 Medical virology I (hepatitis, HIV, herpesviruses)
  • J12 Medical virology II (influenza, respiratory viruses, tick-borne encefalitis)
  • J13 Medical parasitology (basics)
  • J14 Medical mycology (basics)
  • J15 No practical sessions (only exceptionally individual substitutions)
Literature
  • VOTAVA, Miroslav. Lékařská mikrobiologie obecná. (General Medical Microbiology.). 1st ed. Brno: Neptun, 2001, 247 pp. ISBN 80-902896-2-2. info
  • VOTAVA, Miroslav. Lékařská mikrobiologie speciální (Medical microbiology special). 1st ed. Brno: NEPTUN, Březová 18, 637 00 Brno, 2003, 495 pp. ISBN 80-902896-6-5. info
  • VOTAVA, Miroslav, Filip RŮŽIČKA, Vladana WOZNICOVÁ, Lenka ČERNOHORSKÁ, Milada DVOŘÁČKOVÁ, Monika DVOŘÁKOVÁ HEROLDOVÁ, Veronika HOLÁ and Ondřej ZAHRADNÍČEK. Lékařská mikrobiologie: Vyšetřovací metody (Medical Microbiology: Laboratory Procedures). 1st ed. Brno: Neptun, 2010, 495 pp. ISBN 978-80-86850-04-7. info
  • GREENWOOD, David. Lékařská mikrobiologie : přehled infekčních onemocnění : patogeneze, imunita, laboratorní diagnostika a epidemiologie. 1. vyd. Praha: Grada Publishing, 1999, 686 s. ISBN 80-7169-365-0. info
  • RULÍK, Martin, Veronika HOLÁ, Filip RŮŽIČKA, Miroslav VOTAVA, Dana BAUDIŠOVÁ, Jiří GALLO, Simona KAPRÁLOVÁ, Kateřina KOHUŠOVÁ, Dagmar KOUKALOVÁ, Václav KŮDELA, Jiří MIKEŠ, Radko NOVOTNÝ, Martina SIGLOVÁ, Věra TORŠOVÁ and Jiří ZIMÁK. Mikrobiální biofilmy (Microbial Biofilms). 1. vydání. Olomouc: Univerzita Palackého v Olomouci, 2011, 448 pp. Monografie. ISBN 978-80-244-2747-8. info
  • Tituly 1, 2, přednášky v IS MU a veškeré materiály z praktik jsou základní studijní materiály ke zkoušce, ostatní jsou doplňkové.
Teaching methods
practical training in lab
self-study with use of e-learning materials
Assessment methods
Conditions for credits:
(1) absolving all practicals, with following notes:
- no substitution is needed for one justified absence + one without official justification
- in case of more justified absences some form of substitution (e. g. some homework) is needed
- not justified absences (except that one "sick day") are not allowed
(2) complete laboratory report (signature of teacher is not necessary)
(3) succesfully written final test (usually 10 multiple choice questions, each for one point; 7 points needed)
(4) all ROPOT questionaires completed succesfully; if they are always fulfilled in time (prior to particullar lab session), the student has a bonus of one point for final test
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
Information on completion of the course: Podmínky udělení zápočtu viz Doplňkové údaje o předmětu
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2000, Spring 2001, Spring 2002, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2019, recent)
  • Permalink: https://is.muni.cz/course/med/spring2019/VLLM0421c