LF:aVLHE0221c Histology and Embryology I -pr

aVLHE0221c Histology and Embryology I - practice

Faculty of Medicine
spring 2020

Extent and Intensity

0/3/0. 3 credit(s). Type of Completion: z (credit).

Teacher(s)

Guaranteed by

doc. MVDr. Aleš Hampl, CSc.
Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine
Contact Person: doc. MUDr. Miroslava Sedláčková, CSc.
Supplier department: Department of Histology and Embryology – Theoretical Departments – Faculty of Medicine

Timetable of Seminar Groups

aVLHE0221c/30: Mon 17. 2. to Fri 15. 5. Fri 7:30–10:00 F01B1/122
aVLHE0221c/31: Mon 17. 2. to Fri 15. 5. Fri 7:30–10:00 F01B1/122
aVLHE0221c/32: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 F01B1/122
aVLHE0221c/33: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 F01B1/122
aVLHE0221c/34: Mon 17. 2. to Wed 13. 5. Wed 7:30–10:00 F01B1/122
aVLHE0221c/35: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 F01B1/122
aVLHE0221c/36: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 F01B1/122
aVLHE0221c/37: Mon 17. 2. to Mon 11. 5. Mon 7:30–10:00 F01B1/122
aVLHE0221c/38: Mon 17. 2. to Thu 14. 5. Thu 14:00–16:30 F01B1/122
aVLHE0221c/39: Mon 17. 2. to Thu 14. 5. Thu 14:00–16:30 F01B1/122

Prerequisites (in Czech)

aVLAN0121s Anatomy I -sem. && aVLBI0121c Biology I - pract.

Course Enrolment Limitations

The course is only offered to the students of the study fields the course is directly associated with.

fields of study / plans the course is directly associated with

General Medicine (eng.) (programme LF, M-VL)
General Medicine (programme LF, M-VL) (2)

Course objectives

Course objectives
At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs.
5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.

Learning outcomes

At the end of the course students should be able to:
1. Describe tissue processing for light and electron microscopy.
2. Understand and explain the structure of a cell, basic structural principles and function of connective tissues, features of epithelial tissue, structure of different types of muscle tissue, structure and function of nervous tissue.
3. Identify tissue types in histologic slides.
4. Characterize microscopic structure of some organs of human body and explain correlations of tissues within organs.
5. Describe gamete development, explain principle of meiosis and regulation of gametogenesis.
6. Define the early embryonic development as well as development of fetal membranes, placenta and umbilical cord.
7. Characterize stages of embryonic development.

Syllabus

1. Introduction, organization of practicals, teaching aids. Overview of histological methods. Tissue processing for light and electron microscopy - explanation and film. Basic histochemistry and immunocytochemistry.
2. Cytology: Ultrastructure of the cell nucleus during interphase. Ultrastructure of cell organelles I (mitochondria, the Golgi apparatus, the endoplasmic reticulum, ribosomes, lysosomes).
3. Ultrastructure of cell organelles II (peroxisomes, and the centriol). Cell inclusions. The arrangement of cell surfaces: apical, lateral, and basal ones. Intercellular junctions: adhering, occluding, and communicating ones.
4. General embryology I. Aids: Set of embryological schemes and pictures (I).
5. General embryology II. Aids: Set of embryological schemes and pictures (II).
6. General histology: Use of the light microscope, common faults in microscopy. Results of basic staining methods in histology: Hematoxylin-eosine /HE/ (hepar), hematoxylin–eosine-saffron /HES/ (esophagus), AZAN (prostate), impregnation (cerebellum). Connective tissue proper - classification and examples. Slides: Funiculus umbilicalis, Esophagus, Posterior segment of the eye, Lien, Aorta.
7. Supporting tissues: cartilage and bone. Histogenesis of the bone tissue (ossification). Slides: Trachea, Auricula, Elastic cartilage, Lamellar bone, Chondrogenic ossification.
8. Epithelial tissue: epithelial membranes (covering epithelia) and glandular epithelium. Slides: Ren, Vesica fellea, Trachea, Esophagus, Ureter, Palpebra, Skin from the finger tip.
9. Glandular epithelium. Skides: Intestinum tenue, Glandula parotis, Glandula submandibularis.
10. Nerve tissue. Slides: Cortex cerebri, Cerebellum, Medulla spinalis, Ganglion spinale, Peripheral nerve, Motor end plate.
11. Muscle tissue. Slides: Apex linguae, Cardiac muscle tissue Intestinum tenue.
12. Blood cells: Preparation of blood smears and their staining. Cytology of formed elements of blood. Differential white cell count (dWCC) - average values, abnormities and terminology of dWCC. Slide: A smear of peripheral blood stained by Pappenheim's method. Credits.

Literature

recommended literature

VAŇHARA, Petr, Miroslava SEDLÁČKOVÁ, Irena LAUSCHOVÁ, Svatopluk ČECH and Aleš HAMPL. Guide to General Histology and Microscopic Anatomy. 1st ed. Brno: Masarykova univerzita, 2017. ISBN 978-80-210-8453-7. info
MESCHER, Anthony L. Junqueira's basic histology : text and atlas. 13th ed. New York: McGraw-Hill Medical, 2013, xi, 544. ISBN 9781259072321. info

not specified

EROSCHENKO, Victor P. Di Fiore's atlas of histology with functional correlations. 9th ed. Philadelphia: Lippincott Williams-Wilkins, 2000, xv, 363. ISBN 0683307495. info

Teaching methods

Content of this course includes study of slides and electron micrographs in microscopic hall as well as class discussion on the base of materials in the text book. Electronic textbooks are presented also in: http://www.med.muni.cz/histol/atlas.htm

Assessment methods

Testing of knowledge: Student must prove sufficient level of knowledge by written test examination. Each student completes 4 partial tests during semester. Tests are evaluated by point for correct answer. More than half number of correct answers (points) is evaluated as "Passed". All of these tests must be successful. In case of failure, only 1 resit is possible. There is condition 4 from 4 (ie. 4 P / 4 regular tests) or 4 from 5 (ie. 3 P / 4 regular tests + 1 P / 1 resit). If student does not fulfill this condition, credit test follows in the relevant exam period. This test covers all topics studied during semester. In case of failure in credit test, credit will not be given and student must enroll the course again.
Conditions for obtaining credit: 1. Attendance at all practical exercises (100% participation, all absences must be regularly excused (in IS) and substituted).
2. Successful completion of all tests.
3. Submission of all protocols (correctly completed forms of protocols signed by teacher).

Language of instruction

English

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 45.

Listed among pre-requisites of other courses

The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.

Enrolment Statistics (spring 2020, recent)
Permalink: https://is.muni.cz/course/med/spring2020/aVLHE0221c

LF:aVLHE0221c Histology and Embryology I -pr - Course Information

aVLHE0221c Histology and Embryology I - practice

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