LF:BLMT0211p Toxicological Techniques - lec - Course Information
BLMT0211p Toxicological Techniques - lecture
Faculty of Medicinespring 2021
- Extent and Intensity
- 2/0/0. 3 credit(s). Type of Completion: zk (examination).
- Teacher(s)
- Mgr. Andrea Brzobohatá, Ph.D. (lecturer)
Michaela Gregorovičová (assistant) - Guaranteed by
- Mgr. Andrea Brzobohatá, Ph.D.
Department of Forensic Medicine – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Mgr. Andrea Brzobohatá, Ph.D.
Supplier department: Department of Forensic Medicine – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine - Timetable
- Mon 1. 3. 10:00–11:40 ÚSL N01902, Mon 8. 3. 10:00–11:40 ÚSL N01902, Mon 15. 3. 10:00–11:40 ÚSL N01902, Mon 22. 3. 10:00–11:40 ÚSL N01902, Mon 29. 3. 10:00–11:40 ÚSL N01902, Mon 12. 4. 10:00–11:40 ÚSL N01902, Mon 19. 4. 10:00–11:40 ÚSL N01902, Mon 26. 4. 10:00–11:40 ÚSL N01902, Mon 3. 5. 10:00–11:40 ÚSL N01902, Mon 10. 5. 10:00–11:40 ÚSL N01902, Mon 17. 5. 10:00–11:40 ÚSL N01902, Mon 24. 5. 10:00–11:40 ÚSL N01902, Mon 31. 5. 10:00–11:40 ÚSL N01902, Mon 7. 6. 10:00–11:40 ÚSL N01902
- Prerequisites (in Czech)
- BLLC0111s Med.Chem.-seminar
- Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
- fields of study / plans the course is directly associated with
- Medical Laboratory Technologist (programme LF, B-LABD)
- Laboratory Assistant (programme LF, B-SZ)
- Course objectives
- The aim of this subject is to acquaint students with principles of toxicological examining methods used in clinical, industrial, hygienic and pharmacological toxicology. They are physiochemical methods used for proof and determination of toxic substances, medicaments, eventually their metabolites in stomach and intestine content, in bodily secretions and excretions, in all bodily fluids and organs with the aim to assess the cuase of poisoning.
- Learning outcomes
- acquiring knowledge of clinical toxicology, possibilities of examining biological material, drug detection and inclusion in patient diagnosis
- Syllabus
- Toxicity of the substance; Principles of the Good laboratory practice; Principles of the proper sampling, transporting and preservation of biological symplex; Metabolic pathways of substances, pharmacokinetics and pharmacodynamics ; Biological material treatment for toxicological analysis (types of the biological material, sensory examination, deproteinisation, isolation – elimination of balast substances, transfer to the organic phase, preconcentration, diffusion methods, mineralisation, vapour distillation, atc.); Volatile substances, determination of ethanol, head-space – methods, metabolites; Inorganic substances, acids, bases, metalic substances, cyanides, carbon monooxide; Extractable substances, medicaments, drugs of abuse, agrochemicals, evidence; Principles of the isolation (separation) methods (extraction liquid-liquid, solid-phase extraction, microextraction, effect of pH, hydrolysis); Methods of isolation of unknown noxa (toxic, narcotic and psychotropic substances in biological material); Principles of immuno-assay tests for proof of unknown toxic substance (Systematic toxicological analysis, immunochemistry, TLC, GC/MS, HPLC); Principles of substance identification, GC/MS, derivatization, target analysis; Principles of methods for determination of concentrations of medicaments, volatile toxic substances, narcotic and psychotropic substances (GC, HPLC, CE, specific detectors, standards, atc.); Biological and exposure tests (testing of substance toxicity, testing on animals, determination of noxa metabolites in urine, resp. in blood related to exposition to some substances in workplace; Profession testing, cut-off – values; Alternative materials (hair, saliva, sweat, meconium); Biological proof of toxic substance; Interferences in toxicological practice (immunochemistry, cross-reactivity, physiological levels of endogenous substances: hippuric acid, urea, foodstuff additives, phthalates, disinfection artefacts, poppy diet); Internal and external quality control in toxicology (validation of methods, precision, accuracy, reproducibility, cut-off – values, internal standards, comparative blank samples, skill testing – unknown sample, proof by two independent methods at least); Laboratory calculations in toxikology; Statistical data treatment in toxikology; Fundamentals of method establishment in toxicology ;
- Literature
- HIRT, Miroslav, Michal ZELENÝ, Tomáš VOJTÍŠEK, Jan KRAJSA, Andrea BRZOBOHATÁ and Eva MATĚJŮ. Soudní lékařství. Brno: Masarykova Univerzita Právnická fakulta, 2008, 82 pp. Edice učebnic PrF MU č. 413. ISBN 978-80-210-4583-5. info
- Teaching methods
- lecture
- Assessment methods
- examination
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
Information on the extent and intensity of the course: 30. - Listed among pre-requisites of other courses
- Enrolment Statistics (spring 2021, recent)
- Permalink: https://is.muni.cz/course/med/spring2021/BLMT0211p