VLBC0422c Biochemistry II

Faculty of Medicine
spring 2021
Extent and Intensity
0/3/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. Mgr. Zdenka Fohlerová, Ph.D. (seminar tutor)
Mgr. David Galuška (seminar tutor)
Mgr. Jana Gregorová, Ph.D. (seminar tutor)
MUDr. Miroslava Hlaváčová, Ph.D. (seminar tutor)
MUDr. Michaela Králíková, Ph.D. (seminar tutor)
RNDr. Hana Paulová, CSc. (seminar tutor)
doc. Mgr. Ondřej Peš, Ph.D. (seminar tutor)
Mgr. Jiří Slanina, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
Mgr. Marie Tomandlová, Ph.D. (seminar tutor)
Mgr. Jan Víteček, Ph.D. (seminar tutor)
Mgr. Martina Drabinová (assistant)
MUDr. Lenka Gescheidtová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. RNDr. Michal Řiháček, Ph.D., EuSpLM (assistant)
doc. RNDr. Jiří Dostál, CSc. (alternate examiner)
prof. RNDr. Eva Táborská, CSc. (alternate examiner)
doc. RNDr. Josef Tomandl, Ph.D. (alternate examiner)
Guaranteed by
prof. RNDr. Eva Táborská, CSc.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
VLBC0422c/01: Fri 11:00–13:30 A16/216, O. Peš
VLBC0422c/02: Fri 11:00–13:30 A16/211, J. Slanina, J. Smutná
VLBC0422c/03: Fri 11:00–13:30 A16/212, M. Králíková
VLBC0422c/04: Mon 11:00–13:30 A16/216, O. Peš
VLBC0422c/05: Mon 11:00–13:30 A16/211, Z. Fohlerová
VLBC0422c/06: Mon 11:00–13:30 A16/212, J. Gregorová
VLBC0422c/07: Wed 14:00–16:30 A16/211, J. Gregorová
VLBC0422c/08: Wed 14:00–16:30 A16/216, J. Víteček
VLBC0422c/09: Wed 14:00–16:30 A16/212, M. Králíková
VLBC0422c/10: Mon 8:00–10:30 A16/211, J. Smutná
VLBC0422c/11: Mon 8:00–10:30 A16/212, D. Galuška
VLBC0422c/12: Mon 8:00–10:30 A16/216, J. Gregorová
VLBC0422c/13: Mon 14:00–16:30 A16/211, J. Smutná
VLBC0422c/14: Mon 14:00–16:30 A16/212, Z. Fohlerová
VLBC0422c/15: Mon 14:00–16:30 A16/216, O. Peš
VLBC0422c/16: Wed 11:00–13:30 A16/211, J. Smutná
VLBC0422c/17: Wed 11:00–13:30 A16/212, J. Víteček
VLBC0422c/18: Wed 11:00–13:30 A16/216, M. Králíková
Prerequisites (in Czech)
( VLBI0222p Biology II-lect. || VSBI0222p Biology II-lect. ) && VLBF011p Biophysics-lect. && VLBC0321s Biochemistry I sem && VLBC0321c Biochemistry I && VLFY0321c Physiology I - practice && VLFY0321s Physiology I - seminar
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Course is complementary to VLBC0421p a VLLC0421s. The aim of the course it to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics.
Learning outcomes
The student will explain the ways of obtaining biological material for biochemical analysis and is familiar with the rules of manipulation. Describes the physiological values of the most important biochemical parameters. Interprets the results of biochemical analyzes, discusses the significance of the findings. Understands the principles of the most important biochemical-analytical methodologies. Is oriented in basic laboratory manipulations.
Syllabus
  • • Directions for laboratory work. Handling biological material. Laboratory examination. Blood, plasma, serum. Blood collection. Blood processing. Manual methods, pipetting. Biochemical analyzers. Test requisition forms. • Investigations of glucose metabolism. Diabetes mellitus, Enzymatic determination of plasma glucose. Determination of glycaemia by personal glucometer. Oral glucose tolerance test (oGTT). Detection and determination of glucose in urine. Detection of ketone bodies in urine. Determination of glycated hemoglobin (HbA1c). • Investigation of lipid and cholesterol metabolism. Enzymatic determination of serum total cholesterol. Determination of blood cholesterol using Accutrend. Determination of blood triacylglycerols using Accutrend. Determination of HDL-cholesterol and LDL-cholesterol. Calculation of LDL-cholesterol and derived parameters. • Investigations in liver disease I – Enzymes. Determination of catalytic concentrations of aminotranferases in serum. Determination of ALP catalytic concentration in serum. • Selected investigations in liver disease II – Bile pigments and albumin. Determination of total bilirubin in serum. Detection of bilirubin in urine. Detection of urobilinogens in urine. Determination of serum albumin. • Investigation of plasma proteins. Determination of total protein by the biuret test. Determination of albumin in serum. Electrophoresis of serum proteins. Assay for increased level of C-reactive protein. Determination of serum IgE by ELISA method. • Biochemical tests of renal function – I. Investigation of urine by inspection and physical methods. Determination of creatinine in serum and urine. Estimation of glomerular filtration rate from creatinine clearance. Fractional reabsorption and excretion of water. • Biochemical tests of renal function – II. Detection of proteinuria. Urine test for haem compounds. Determination of protein concentration in urine. Detection of microalbuminuria. Detection of Bence Jones protein in urine. Determination of urea in serum and urine. Approximate assessment of the nitrogen balance. • Routine urinalysis. Test strips. Multipurpose diagnostic strips in urinalysis. Detection of addictive drugs in urine. Detection of barbiturates in urine. Detection of luteinizing hormone in urine. • Examinations in urolithiasis. Solubility of uric acid. Detection of uric acid by murexide reaction. Simplified chemical analysis of renal calculi. Determination of uric acid in serum and urine. • Some investigations of the gastrointestinal tract. Selected examinations of stomach functions. Laboratory tests in pancreas diseases. Test of occult bleeding in gastrointestinal tract.
Literature
    required literature
  • Podklady ke cvičení dostupné v Informačním systému
Teaching methods
laboratory practicals
Assessment methods
Practicals in biochemistry lab.
Full attendance in all lessons (making up all missing and justified lessons. Obtaining of course-unit credits of practices is the pre-requisite for registration to the examination of Biochemistry II.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
Information on the extent and intensity of the course: 45.
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (spring 2021, recent)
  • Permalink: https://is.muni.cz/course/med/spring2021/VLBC0422c