ZLBC0422c Biochemistry II -practice

Faculty of Medicine
spring 2022
Extent and Intensity
0/1/0. 1 credit(s). Type of Completion: z (credit).
Teacher(s)
doc. PharmDr. Jiří Kos, Ph.D. (seminar tutor)
Mgr. Jindra Smutná, Ph.D. (seminar tutor)
doc. RNDr. Josef Tomandl, Ph.D. (seminar tutor)
doc. RNDr. Jiří Dostál, CSc. (assistant)
Mgr. David Galuška (assistant)
Mgr. Jana Gregorová, Ph.D. (assistant)
Martina Hanousková (assistant)
MUDr. Michaela Králíková, Ph.D. (assistant)
Miriam Minaříková (assistant)
RNDr. Hana Paulová, CSc. (assistant)
doc. Mgr. Ondřej Peš, Ph.D. (assistant)
Mgr. Jiří Slanina, Ph.D. (assistant)
Bc. Ivana Ševčíková (assistant)
Mgr. Marie Tomandlová, Ph.D. (assistant)
Guaranteed by
doc. RNDr. Josef Tomandl, Ph.D.
Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Contact Person: RNDr. Hana Paulová, CSc.
Supplier department: Department of Biochemistry – Theoretical Departments – Faculty of Medicine
Timetable of Seminar Groups
ZLBC0422c/21: Thu 24. 2. 8:00–9:40 A16/211, Thu 17. 3. 8:00–9:40 A16/211, Thu 7. 4. 8:00–9:40 A16/211
ZLBC0422c/22: Wed 23. 2. 8:00–9:40 A16/211, Wed 16. 3. 8:00–9:40 A16/211, Wed 6. 4. 8:00–9:40 A16/211
ZLBC0422c/23: Thu 3. 3. 8:00–9:40 A16/211, Thu 24. 3. 8:00–9:40 A16/211, Thu 14. 4. 8:00–9:40 A16/211
ZLBC0422c/24: Wed 2. 3. 8:00–9:40 A16/211, Wed 23. 3. 8:00–9:40 A16/211, Wed 13. 4. 8:00–9:40 A16/211
Prerequisites (in Czech)
( ZLBC0321s Biochemistry I - seminar || ZLBC0221s Biochemistry I - seminar ) && ZLFY0321s Physiology I - sem. && ZLBI0222p Biology II-lect.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course is complementary to ZLBC0422p. The aim of the course is to bring experience with solving practical problems in clinical biochemistry, to acquire practical laboratory skills and to learn students to use results of biochemical analysis for diagnostics
Learning outcomes
After completing the course, the student will be able to:
- manipulate basic laboratory aids and follow the principles of safe work in the laboratory
- explain the methods of obtaining biological material for biochemical analyzes and is acquainted with the rules of handling it.
- interpret the results of biochemical analyzes and discuss the significance of the findings.
- understand the principles of the most important biochemical-analytical methodologies.
Syllabus
  • Organization of work in practical exercises.
  • Enzymatic determination of plasma glucose. Determination of glycemia by personal glucometer. Detection and determination of glucose in urine. Evidence of urinary ketone bodies.
  • Determination of calcium in serum. Determination of catalytic activity of total ALP and bone isoenzyme in serum.
  • Examination of saliva secretion rate. Determination of saliva buffering capacity. Identification of selected organic substances in saliva. Detection of amylase in saliva and its substrate specificity.
Literature
    required literature
  • Návody ke cvičením dostupné v IS MUNI.
Teaching methods
Laboratory practical classes
Assessment methods
Course-unit credit.
Conditions for giving the course-unit credit (all conditions should be met):
  • completion of all lessons,
  • elaboration of all protocols and insertion into the Homework vaults in IS MUNI.
    The detailed conditions are posted in Information System (Study materials – Course-related instructions).
    • Language of instruction
    Czech
    Further comments (probably available only in Czech)
    The course is taught annually.
    General note: Úspěšné ukončení předmětu je podmínkou přístupu ke zkoušce v předmětu ZLBC0422p.
    Information on the extent and intensity of the course: 15.
    Listed among pre-requisites of other courses
    Teacher's information
    https://is.muni.cz/auth/el/1411/jaro2022/ZLBC0422c/
    Information to practical exercises, the conditions for giving the course-unit credit and the syllabus are available in the IS MUNI in the Course-related instructions. The syllabus is also posted on the notice board of the Department of Biochemistry.

    A white coat, slippers or sturdy shoes with overshoes, a respirator type FFP2, gloves, a calculator are required.

    Consultations are possible after a personal arrangement with the teacher.

    The course is also listed under the following terms spring 2020, spring 2021, spring 2023, spring 2024, spring 2025.
    • Enrolment Statistics (spring 2022, recent)
    • Permalink: https://is.muni.cz/course/med/spring2022/ZLBC0422c