EMIT0211p Instrumental technique

Faculty of Medicine
spring 2025
Extent and Intensity
2/0/0. 5 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
RNDr. Miroslava Beňovská, Ph.D. (lecturer)
doc. MUDr. Milan Dastych, CSc., MBA (lecturer)
doc. MVDr. Aleš Hampl, CSc. (lecturer)
Mgr. Alice Hoffmannová, Ph.D. (lecturer)
RNDr. Alena Mikušková (lecturer)
Ing. Martina Podborská, Ph.D. (lecturer)
Mgr. Ondřej Wiewiorka, Ph.D. (lecturer)
MUDr. Ing. David Zeman, Ph.D. (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
doc. MUDr. Milan Dastych, CSc., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: doc. MVDr. Aleš Hampl, CSc.
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
“The aim of the theoretical part of the preparation is to provide a theoretical foundation of the physical principles used in laboratory and analytical techniques. It aims to familiarize students in detail with the application of these physical principles in specific instruments, including existing modifications in the current equipment of medical laboratories. By examining specific cases, students will observe various variants and modifications, understanding their purpose, advantages, and critical points. They will assess the advantages, disadvantages, and limitations of individual instrumental and analytical systems. Students will apply the acquired theoretical knowledge to practical activities in the laboratory and follow standard technical operating procedures.”
Learning outcomes
"After completing the course, the student will be able to: Navigate new instrumental technologies; Understand the technical parameters of laboratory instruments and compare them with each other; Assess the advantages, disadvantages, and limitations of individual instrumental and analytical systems; Apply the acquired theoretical knowledge to practical activities in the laboratory."
Syllabus
  • - Basic physical principles used in laboratory techniques; - Optical methods; - Electrochemical methods; - Ion-selective electrodes; - Cryoscopy, Ebullioscopy; - Isotopes; - Centrifugation; - Demonstrations of basic physical principles used in laboratory techniques; - Spectrophotometer construction (light sources, monochromators, interference filters, absorption environment, application of physical principles in laboratory techniques); - UV/VIS spectrophotometer, atomic absorption spectrophotometer; pH meter, polarograph, coulometer, conductometer; - Electrophoresis equipment; - Thin-layer chromatography equipment, liquid chromatograph – HPLC, gas chromatograph, detectors; - Osmometer; - ISE (Na, K, Cl, Ca, Mg); - Clark oxygen electrode, Severinghaus pO2 electrode, enzyme electrodes; - Centrifuges; - Thermostats, water baths; - Equipment for RIA techniques; - Luminometer.
Literature
  • DASTYCH, Milan. Instrumentální technika : obor zdravotní laborant. 2., dopl. vyd. Brno: Masarykova univerzita, 2014, 201 s. ISBN 9788021071032. info
Teaching methods
“Lecture using audiovisual teaching aids (PowerPoint presentations, narrated videos) including interactive inputs.”
Assessment methods
written test
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms spring 2021, spring 2022, spring 2023, spring 2024.
  • Enrolment Statistics (spring 2025, recent)
  • Permalink: https://is.muni.cz/course/med/spring2025/EMIT0211p