MBTS081c Transfusion services - practices

Faculty of Medicine
spring 2025
Extent and Intensity
0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
RNDr. Libuše Janků (seminar tutor)
MUDr. Hana Lejdarová, Ph.D., MBA (seminar tutor)
MUDr. Zdeňka Čermáková, Ph.D. (assistant)
Michaela Gregorovičová (assistant)
Guaranteed by
MUDr. Hana Lejdarová, Ph.D., MBA
Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Michaela Gregorovičová
Supplier department: Department of Laboratory Methods – Departments of Non-medical Branches – Faculty of Medicine
Prerequisites
master's studies focused on laboratory medicine or natural sciences
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
In the practical part of the course, the goal is to acquire practical skills of an extent that will allow the graduate to be easily included in laboratory teams of laboratories in which immunohematological laboratory performances and / or work teams are carried out in ZTS where the production of transfusion products (auto and allogeneic). Emphasis is placed on gaining the capabilities of focused attention and precision and awareness of responsibility for your work. The aim is for graduates to master the basics of safety and health at work with biological materials and to adopt the ethics of a health worker.
Learning outcomes
Upon completion of the course the student will be able to practically perform, control and release the results of immunohematological examinations including HLA examination, evaluate the quality of the analytical sample, use laboratory information systems, know and observe the principles of good laboratory practice and good manufacturing practice, participate in the production of quality management documents, communicate with clinicians.
Syllabus
  • Blood group examination in the AB0 system, examination of weak blood groups and abnormalities, excretion, Rh blood group examination, examination of weak and variant D antigens, importance of interpretation of patients and blood donors, immunoprophylaxis, examination of antierythrocytic antibodies, methods of detection, screening, determination of specificity of antierythrocyte antibodies, resolution of mixtures, quantitative determination, importance for the clinic, pre-transfusion examination, obligatory laboratory tests and procedures, laboratory compatibility of transfusions, phenotyping, laboratory determination of antigens erythrocytes using different types of IVD and various methods, immunohematological examination of patients with   AIHA and laboratory transfusions, direct antiglobulin assay, quantitative PAT assay, use of polyspecific and monospecific antiglobulin human (AGH) sera, complementary tests, immunohematological examination of newborn, blood group, PAT, free antibody immuno examination of patients with laboratory-HDN and ensuring transfusion laboratory examination transfusion reactions, resolution, anti-erythrocyte IgG and IgM antibodies using immunohaematological tests
  • Laboratory diagnostics HLA, methods of detection of HLA antigens I. and II. class, antibody detection, organization of work on detection of HLA signs of donor cadaveris organs and related transplant candidates, work with registers
  • Pre-analytical, analytical and postanalytical phases in a laboratory, identification and continuity throughout the laboratory process, preparation of samples for analysis, centrifugation, sorting
  • Laboratory information systems, information systems for ZTS production, on-line and off-line communication, print assemblies and outputs, control system, work with barcodes
  • Work with automatic analyzers, method parameters, reagent preparation, reaction tracking and result processing
  • Systems of internal and external quality control
  • Economics of laboratory operation and ZTS, system of posting of performances and transfusion products to health insurance companies, creation of prices of transfusion products
  • Management documentation of quality management systems in the laboratory and in the ZTS
  • Production of blood transfusion products, work with donors of blood donors, assessment of blood donor eligibility, routine blood sampling, apheresis sampling, obligatory laboratory tests, transfusion markings, storage of transfusion products, dispensing and transport of blood transfusion products, quality control
  • Autologous transfusion products, principles of donor-patient work, methods of collection and processing
Literature
  • Transfuzní lékařství. Edited by Vít Řeháček - Jiří Masopust. 1. vyd. Praha: Grada, 2013, 237 s. ISBN 9788024745343. info
  • PENKA, Miroslav and Eva TESAŘOVÁ. Hematologie a transfuzní lékařství II. 1st ed. Praha: Grada Publishing, 2012, p. 95 - 102. ISBN 978-80-247-3460-6. info
Teaching methods
solving problems from the analytical part of the subject using analyzers, instruments and laboratory equipment in the teaching laboratory,
evaluation and correct interpretation of performed examinations,
continuous monitoring of assigned tasks, possible corrections, corrections and evaluation by the lecturer.
Assessment methods
active practical works participation, - evaluating of acquired knowledge and praxis skills, - fulfilling requirements - written exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.
  • Enrolment Statistics (spring 2025, recent)
  • Permalink: https://is.muni.cz/course/med/spring2025/MBTS081c