MOEB1041p Experimental biophotonics - lecture

Faculty of Medicine
spring 2025
Extent and Intensity
1/0/0. 1 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
Ing. Zbyněk Dostál, Ph.D. (lecturer)
prof. RNDr. Radim Chmelík, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Radim Chmelík, Ph.D.
Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Contact Person: Lenka Herníková
Supplier department: Department of Optometry and Orthoptics – Departments of Non-medical Branches – Faculty of Medicine
Prerequisites
Students are expected to have theoretical knowledge as from "Applied optics" course.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The course provides an overview of modern experimental techniques used to determine structure, function and dynamics of macro and micro biological samples by means of electromagnetic radiation. Attention will be focused on non-invasive methods used for detection, diagnosis and treatment of diseases, including methods used in ophthalmology and optometry.
Learning outcomes
Deeper theoretical and chiefly practical knowledge of some modern techniques that use electromagnetic radiation to study structure, function and dynamics of biological samples and methods used to detect, diagnose and treat diseases.
Syllabus
  • 1. Brief summary (reminding) of fundamentals in: Electromagnetic radiation. Structure of matter. Structure of atom. Structure of molecule. Energy levels of electrons. Absorption and emission of radiation. Spectra of atoms and molecules. Light. X-ray and gamma rays.
  • 2. Brief summary (reminding) of fundamentals in: Propagation of light and its interactions. Electromagnetic waves, photons. Description and properties (interference, diffraction, energy, momentum). Filters of light (interference, neutral, polarizing), filters in ophthalmology. UV filter and glasses.
  • 3. Sources of radiation, lasers (reminding of the priciple). Laser types, use in ophthalmology. Properties of the laser beam. Detectors of radiation (classical and digital camera - principle and applications in medicine). Luxmeter. Detection of UV and IR radiation. Remote TV controls.
  • 4. Interactions of radiation with a matter and how it affects tissues. Absorption, transmission, emission + spectroscopy.
  • 5. Applications in diagnosis and therapeutic methods: tomography, irradiation, "light acupuncture"
  • 6. Optical micromanipulations. Optical tweezers and scalpel.
  • 7. Other practical applications of lasers in medicine (ophthalmology): LASIK, femto-LASIK, surgery, stomatology (dental laser, photoactivation), dermatology, cosmetics, cytometry, microdissection.
  • 8. Brief summary of fundamentals in optical microscopy: bright and dark field, Zernike phase contrast, polarization microscopy, differential interference contrast (DIC), 3D visualization methods.
  • 9. Brief summary of fundamentals in confocal microscopy and OCT/OCM. Holographic microscopy. 3D image formation. Endoscopy.
  • 10. Fluorescence microscopy and its modifications (fluorescence resonance energy transfer - FRET, total internal reflection fluorescence - TIRF), raman spectroscopy, multiphoto microscopy, second harmonic generation - SHG, stimulated emission depletion - STED, structured illumination microscopy - SIM, light-sheet microscopy.
  • 11. Photodynamic therapy, optical biosensors.
  • 12. Applications of micro and nano opto-technologies in medicine (retinal implants), nanosurgery.
Literature
    recommended literature
  • P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003.
  • J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995.
  • HRAZDIRA, Ivo and Vojtěch MORNSTEIN. Lékařská biofyzika a přístrojová technika. Dotisk 1. vyd. Brno: NEPTUN, 2004, 381 s. ISBN 8090289614. info
  • VLACH, Bohumil and Josef FUKA. Vlnová povaha světla. Vyd. 1. Praha: Státní pedagogické nakladatelství, 1971, 199 s. URL info
  • FUKA, Josef and Bedřich HAVELKA. Optika a atomová fyzika. fyzikální kompendium pro vysoké školy. Vyd. 1. Praha: Státní pedagogické nakladatelství, 1961, 845 s. URL info
Teaching methods
lecture, class discussion
Assessment methods
The credit is awarded on the basis of active participation in teaching.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught: every week.
Information on the extent and intensity of the course: 15.
The course is also listed under the following terms Spring 2014, Spring 2015, Spring 2016, Spring 2017, Spring 2018, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024.
  • Enrolment Statistics (spring 2025, recent)
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