FC3017 Biochemistry Laboratory Training

Faculty of Education
Spring 2022
Extent and Intensity
0/0/2. 3 credit(s). Type of Completion: k (colloquium).
Teacher(s)
Mgr. Petr Ptáček, Ph.D. (seminar tutor)
Guaranteed by
Mgr. Petr Ptáček, Ph.D.
Department of Physics, Chemistry and Vocational Education – Faculty of Education
Contact Person: Jana Jachymiáková
Supplier department: Department of Physics, Chemistry and Vocational Education – Faculty of Education
Timetable of Seminar Groups
FC3017/01: Mon 14. 2. 16:00–19:50 laboratoř 89, Fri 18. 2. 15:00–18:50 laboratoř 89, Mon 21. 2. 16:00–19:50 laboratoř 89, Fri 25. 2. 15:00–18:50 laboratoř 89, Mon 28. 2. 16:00–19:50 laboratoř 89, Fri 4. 3. 15:00–18:50 laboratoř 89, Mon 7. 3. 16:00–19:50 laboratoř 89, Fri 11. 3. 15:00–18:50 laboratoř 89, Mon 14. 3. 16:00–19:50 laboratoř 89, Fri 18. 3. 15:00–18:50 laboratoř 89, Mon 21. 3. 16:00–19:50 laboratoř 89, Fri 25. 3. 15:00–18:50 laboratoř 89, P. Ptáček
Prerequisites (in Czech)
Teoretická znalost problematiky.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Main objectives can be summarized as follows:
to practically prove the basic characteristics of the biochemical compounds and methodical advancements for its study and enzyme analytical improvement
practical performance and prove of qualitative and quantitative reactions of carbohydrate, lipids, amino-acids, proteins, nucleic acids and enzymes
to practically and expertly present own laboratory work and results
Learning outcomes
Upon completion of this course the student will gain:  knowledge of acquiring basic habits and skills in conducting experiments in the biochemistry laboratory and achieving as accurate and accurate as possible the results of practical analyzes of samples  Skills and skills for performing practical experiments in an biochemistry laboratory
Syllabus
  • Qualitative reactions of carbohydrates
  • Carbohydrate thin-layer chromatography
  • Preparation and identification of lipids in egg yolk
  • Verification and chromatography separation of leaf pigments
  • Characteristic reactions of amino acids and proteins
  • Verification of amino acids and peptide
  • Amino acids paper chromatography
  • Separation of proteins
  • Separation and properties of nucleic acids
  • Enzyme reaction temperature dependency
  • Verification of proteins
  • Ezyme substrate specification
  • Enzyme activity determination
  • Kinetics of enzyme reaction
Literature
  • ŠIBOR, Jiří and Hana CÍDLOVÁ. Praktická cvičení z biochemie a bioorganické chemie (Hands-on Training of Biochemistry and Bioorganic Chemistry). 1. vydání. Brno: Masarykova univerzita v Brně, 2005, 49 pp. ISBN 80-210-3883-7. info
Teaching methods
Teaching type: laboratory practice
Assessment methods
Requirements for completion: laboratory practice acquittance
elaboration of laboratory protocols
colloquium
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 8 hodin.
The course is also listed under the following terms Spring 2020, Spring 2021, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2022, recent)
  • Permalink: https://is.muni.cz/course/ped/spring2022/FC3017