F1BC1_16 Biochemistry

Faculty of Pharmacy
Autumn 2024
Extent and Intensity
3/1/0. 6 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
PharmDr. Zuzana Soldánová (lecturer)
Mgr. Petra Herczogová (seminar tutor)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
PharmDr. Zuzana Soldánová (seminar tutor)
Mgr. Ondřej Smištík (seminar tutor)
Guaranteed by
Mgr. Marie Brázdová, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Thu 13:00–15:30 45-135
  • Timetable of Seminar Groups:
F1BC1_16/01: Tue 17. 9. 7:30–10:10 45-229, Tue 8. 10. 7:30–10:10 45-229, Tue 29. 10. 7:30–10:10 45-229, Tue 19. 11. 7:30–10:10 45-229, D. Nykodýmová, O. Smištík
F1BC1_16/02: Tue 24. 9. 7:30–10:10 45-229, Tue 15. 10. 7:30–10:10 45-229, Tue 5. 11. 7:30–10:10 45-229, Tue 26. 11. 7:30–10:10 45-229, P. Herczogová, D. Nykodýmová
F1BC1_16/03: Tue 1. 10. 7:30–10:10 45-229, Tue 22. 10. 7:30–10:10 45-229, Tue 12. 11. 7:30–10:10 45-229, Tue 3. 12. 7:30–10:10 45-229, P. Herczogová, O. Smištík
F1BC1_16/04: Tue 17. 9. 10:25–13:05 45-229, Tue 8. 10. 10:25–13:05 45-229, Tue 29. 10. 10:25–13:05 45-229, Tue 19. 11. 10:25–13:05 45-229, D. Nykodýmová, O. Smištík
F1BC1_16/05: Tue 24. 9. 10:25–13:05 45-229, Tue 15. 10. 10:25–13:05 45-229, Tue 5. 11. 10:25–13:05 45-229, Tue 26. 11. 10:25–13:05 45-229, P. Herczogová, D. Nykodýmová
F1BC1_16/06: Tue 1. 10. 10:25–13:05 45-229, Tue 22. 10. 10:25–13:05 45-229, Tue 12. 11. 10:25–13:05 45-229, Tue 3. 12. 10:25–13:05 45-229, P. Herczogová, O. Smištík
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
The knowledge of subject in range of lectures
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Biochemistry is the study of the chemical processes (changes) which condition and accompany life in its most varied forms and manifestations, and attemps to explain its essence on a molecular level. Medical biochemistry is biochemistry in use, applied quite specifically in veterinary medicine (for example, the peculiarities of the metabolism of nutrients in invidual species of livestock). Further, in teaching of biochemistry, it is necessary to concentrate on detailed interpretation of selected chapters, that are most significant for the formation of the European profile of a graduate of veterinary university. Practical (laboratory) work where verbal information is confirmed by practical experience is also an integral component of the biochemistry teaching.

Learning outcomes
Basic knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology, Pharmacogenomics, Structure and evolution of genomes.
Syllabus
  • Syllabus Biochemistry
    1. Biochemistry: introduction Fundamentals of biochemistry, living systems, prokaryotic/eukaryotic cell, animal/plant cell, organelles, chemical reactions, weak interactions, water medium of life, pH, pKa, buffer systems, osmotic pressure, inorganic substances in organism 2. Structure and function of proteins Amino acids, peptides, structure and function of proteins, hemoglobin and myoglobin 3. Enzymes Properties of enzymes, classification of enzymes, cofactors, catalysis, kinetics of enzyme reactions, enzymes in therapy 4. Biological membranes and membrane transport Membranes - composition, properties, types of membrane transport 5. Bioenergetics and metabolism of saccharides General characteristics, mono, di, polysaccharides, glycosaminoglycans, proteoglycans, lectins, metabolism, macroergic compounds, glucose transport, glucose metabolism glycolysis, pyruvate conversions, gluconeogenesis, glycogen synthesis and degradation, glycogenosis, pentosophosphate pathway, fructose metabolism, galactose, ascorbic acid 6. Metabolism of amino acids Protein degradation, protein sources, amino acid catabolism, synthesis of non-essential amino acids, transformations of the carbon skeleton of amino acids 7. Lipids and lipid metabolism Lipid types and metabolism in general, transport of lipids, fatty acids, metabolism of fatty acids (ß-oxidation and synthesis), ketone bodies, synthesis of triacylglycerols, phospholipids, sphingolipids, lipid peroxidation 8. Cholesterol metabolism and icosanoids Synthesis, transport and excretion of cholesterol, steroid division, icosanoids (characteristics and synthesis) 9. Aerobic metabolism Citrate cycle: acetyl CoA, pyruvate decarboxylation, citrate cycle reaction, citrate regulation. cycle, anaplerotic reactions 10. Respiratory chain, reactive oxygen species: energy transformation, aerobic phosphorylation, NADH + H+, FADH2, transport mechanisms (shuttles), respiratory chain cofactors, respiratory chain enzyme complexes, ATP synthesis, reactive oxygen species, antioxidants 11. Nucleic acids Basic characteristics, structure, DNA/RNA differences, biosynthesis and degradation of purine and pyrimidine nucleotides, disorders of purine metabolism, replication, transcription, translation, genetic code, mutation, glycosylation of proteins, regulation of gene expression 12. Biochemistry of extracellular and intracellular communication Regulation of metabolism, nerve cell, interorgan relationships, biotransformation


    Lecturer: M.Sc. Marie Brázdová, Ph.D., Exercising: PharmDr. Daniela Nykodýnová PharmDr. Dominik Rotrekl M.Sc. Zuzana Soldánová Dr. Ing. Branislav Ruttkay-Nedecky M.Sc. Marie BRAZDOVÁ, Ph.D. Discipline guarantor: M.Sc. Marie Brázdová, PhD.
Literature
    required literature
  • Josef Tomandl, Eva Táborská a kolektiv. Biochemie I : semináře. Brno, 2012. ISBN 978-80-210-5740-1. info
    recommended literature
  • MURRAY,R.K., GRANNER,D.K.,RODWELL,V.W. Harper´s Illustrated Biochemistry. Appleton & Lange, 2006. ISBN 07-147885-X. info
  • Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Ro. Harperova biochemie. ČR, 2002. ISBN 80-7319-013-3. info
  • CHAMPE,P.C.,HARVEY,R.A. Lippincott´s Illustrated Reviews: Biochemistry. Lippincott Wiliams & Wilkins, 1994. ISBN 0-397-51091-8. info
  • MURRAY,R.K., GRANNER,D.K.,MAYES,P.A.,RODWELL,V.W. Harperova biochemie (český překlad). Nakladatelství a vydavatelství H & H, 1993. ISBN 80-85787-38-5. info
  • APPS,D.K.,COHEN,B.B.,STEEL,C.M. Biochemistry (A concise text for medical students). Bailliére Tindall, 1992. ISBN 70-20-1444-3. info
Teaching methods
lectures, class discussion, laboratory work, homework,
Assessment methods
Successful completion of the course with a written and oral exam. The grade depends mainly on the written part, which consists of a test with 40 questions, 150 points. To pass the course successfully, you must pass the test at 60%. The prerequisite for passing the exam is passing all practical exercises, passing the test from the exercises with 80%, adequate knowledge of biochemical issues and correct preparation of all exercise protocols. At the same time, a short task readiness test will be conducted before each exercise. During the course of study, it is mandatory to complete 4 tests from basic biochemical pathways. Furthermore, the group task of developing 2 chapters of seminars on Biochemistry (Josef Tomandl, Eva Táborská and the team. Biochemistry I : seminars. Brno, 2012. ISBN 978-80-210-5740-1). The written examination test is prepared on the basis of lectures and questions from Biochemistry I-seminar 2012 - see recommended literature. He will then make up the teaching at the time and in the manner determined by the relevant teacher.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Student requirements Successful completion of the course with a written and oral exam. The grade depends primarily on the written part of the test. To pass the course successfully, you must pass the test at 60%. Requirements for the student's readiness for practical teaching, conditions for passing the test from the practical part of the teaching. During the semester, students are required to demonstrate their readiness for practical teaching according to the requirements set by the head of the discipline at the beginning of the semester. The prerequisite for mastering the subject "BIOCHEMISTRY" is the completion of all practical exercises, adequate knowledge of biochemical issues and the correct preparation of all exercise protocols. At the same time, a short task readiness test will be conducted before each exercise. During the course of study, it is mandatory to complete 4 tests from basic biochemical pathways. The written test is prepared on the basis of lectures and questions from Biochemistry I-Seminars - see recommended literature (Tomandl et al. 2012). In case of poor-quality preparation, the student will be excluded from the exercise, as it could immediately endanger the course of the exercise or cause harm to the health of any of the participants of the practical training. He will then make up the teaching at the time and in the manner determined by the relevant teacher. If the student fails the test, he/she will take the test on a date set by the relevant practical teacher. Absences from practical classes must be substantiated by valid documents. He is responsible for the technical content of the practical exercises in biochemistry, and the guarantor of the discipline ensures the material security and operation of the practical exercises.
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2022, Autumn 2023.
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