FaF:F1EP1_12 Explant Cultures - Course Information
F1EP1_12 Explant Cultures
Faculty of PharmacySpring 2025
- Extent and Intensity
- 1/1/0. 3 credit(s). Type of Completion: zk (examination).
In-person direct teaching - Teacher(s)
- Ing. Marcela Nejezchlebová (seminar tutor)
prof. PharmDr. Petr Babula, Ph.D. (lecturer) - Guaranteed by
- prof. PharmDr. Petr Babula, Ph.D.
Department of Natural Drugs – Departments – Faculty of Pharmacy
Supplier department: Department of Natural Drugs – Departments – Faculty of Pharmacy - Prerequisites
- FAKULTA(FaF) || OBOR(MUSFaF)
Fundamentals of Plant Physiology, Pharmaceutical Botany, Laboratory practice in the analytical laboratory. - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12 - fields of study / plans the course is directly associated with
- Pharmacy (programme FaF, M-FARM)
- Multidisciplinary studies at Faculty of Pharmacy (programme CST, KOS)
- Course objectives
- Subject includes topics implying plant growth regulators and their physiological effects, explants cultures, basic methodes of fluorescent microscopy for work with explants cultures.
- Learning outcomes
- Understanding of basic processes, experiments, and exploitation of explant cultures.
- Syllabus
- Lectures:
1. Introduction to plant cultures ? from back to future.
2. Culture media and their composition, preparation and sterilization of explants.
3. Types of explant cultures
4. Initiation and maintenance of callus and suspension cultures
5. Characterization of growth and development of callus and suspension cultures.
6. Protoplast cultures ? isolation of protoplast, protoplast cultivation and characterization.
7. Protoplast technologies ? fusion and selection.
8. Selection of plant cells for desiderable characteristics ? mutant selection, cold tolerance, salt tolerance, disease/toxin resistance.
9. Aspects of plant regeneration. Micropropagation.
10. Cryopreservation and artificial seeds.
11. Secondary metabolites from plant in vitro cultures I.
12. Secondary metabolites from plant in vitro cultures II.
13. Transgenic plants in pharmacy.
Practical classes:
1. Plant growwth regulators - biotests
2. Preparation of cultivation media, establishing of plant cell tissue cultures
3. Determination of growth and biosynthetical parameters of plant tissue cultures
4. Methods of fluorescence microscopy in plant cell cultures
- Lectures:
- Literature
- Teaching methods
- Lectures, practical classes
- Assessment methods
- Written examination
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
- Teacher's information
- 100% of attendance on practical classes
- Enrolment Statistics (Spring 2025, recent)
- Permalink: https://is.muni.cz/course/pharm/spring2025/F1EP1_12