FaF:F1MG1_16 Methods of Molecular Biology - Course Information
F1MG1_16 Methods of Molecular Biology
Faculty of PharmacyAutumn 2024
- Extent and Intensity
- 1/0/0. 2 credit(s). Type of Completion: zk (examination).
In-person direct teaching - Teacher(s)
- doc. RNDr. Jan Hošek, Ph.D. (lecturer)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Daniel Renčiuk, Ph.D. (lecturer) - Guaranteed by
- doc. RNDr. Jan Hošek, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy - Timetable
- each odd Thursday 8:00–9:40 45-135
- Prerequisites
- FAKULTA(FaF) || OBOR(MUSFaF)
Molecular biology.The knowledge of subject in range of lectures. - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 100 student(s).
Current registration and enrolment status: enrolled: 94/100, only registered: 0/100 - fields of study / plans the course is directly associated with
- Pharmacy (programme FaF, M-FARM)
- Multidisciplinary studies at Faculty of Pharmacy (programme CST, KOS)
- Course objectives
- The aim of the course is to interpret the basic principles of molecular biology methods and to practice the basic operations in the molecular biology used.
- Learning outcomes
- The aim of this subject is to provide students with basic information from molecular biology methods. The ground of lectures is focused on structure of proteins and nucleic acids and functions and relationships of these informational macromolecules in transition of genetic information.
- Syllabus
- 1) Isolation and characterisation of nucleic acids and proteins
2) Centrifugation
3) Electrophoresis - agarose, polyacrylamide, pulse-field, capillary
4) Modifying enzymes
5) Restriction endonucleases
6) Polymerase chain reaction including real-time PCR, Real-time PCR
7) Southern a northern blotting, hybridisation, DNA and RNA chips
8) Sequencing of DNA and proteins
9) ELISA, western blotting proteomics
10) Transfection
11) Analysis of protein-protein interactions,
12) Analysis of DNA-protein interaction 13) Reporter systems
- 1) Isolation and characterisation of nucleic acids and proteins
- Literature
- required literature
- Šmarda J, et al. Metody molekulární biologie. Masarykova Universita Brno, 2005. info
- recommended literature
- Barker K. At the Bench. A laboratory navigator. Cold Spring Harbor Laboratory Press, USA, 2005. info
- Teaching methods
- lectures, videos
- Assessment methods
- Written exam, to successfully complete the need to master 60%.
- Language of instruction
- Czech
- Further Comments
- Study Materials
The course can also be completed outside the examination period.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/pharm/autumn2024/F1MG1_16