FAAO2_12 Antioxidants and Free Radicals - practical classes

Faculty of Pharmacy
Autumn 2024

The course is not taught in Autumn 2024

Extent and Intensity
0/1/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching
Teacher(s)
doc. PharmDr. Renata Kubínová, Ph.D. (seminar tutor)
doc. Mgr. Jan Muselík, Ph.D. (seminar tutor)
Mgr. Ing. Jiří Václavík, Ph.D. (seminar tutor)
Guaranteed by
doc. PharmDr. Renata Kubínová, Ph.D.
Department of Natural Drugs – Departments – Faculty of Pharmacy
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
Fundamentals of analytical chemistry, organic chemistry, physiology and pathophysiology.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this subject is to offer the students with pharmacological aspects oxidative stress, chemistry of free radicals, free radical reactions, antioxidants natural origin, and methods for assesment of oxidive stress and for detection of antioxidant activity of natural compounds.
Learning outcomes
Mastering of methods for testing antioxidant activity, overview of oxidative processes in the body, oxidative stress, and its pathophysiology.
Syllabus
  • 1.Detection of reactive oxygen species
    2.Free- radicals reaction
    3.HPLC-ABTS methods for detection of antioxidant activity of compounds
    4.In vitro screening for antioxidant activity of natural compounds - spectrophotometric methods
    5.In vitro screening for antioxidant activity of natural compounds - luminiscence techniques
    6.Tyrosine nitration assay
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Passing practical exercises, written exam including information corresponding to the content of the syllabus.
  • Permalink: https://is.muni.cz/course/pharm/autumn2024/FAAO2_12