FAMG1_16 Methods of Molecular Biology

Faculty of Pharmacy
Autumn 2024
Extent and Intensity
1/0/0. 2 credit(s). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
doc. RNDr. Jan Hošek, Ph.D. (lecturer)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Daniel Renčiuk, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Jan Hošek, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
each even Tuesday 12:00–13:40 44-037
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
FAME1_16 Molecular Biology
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 30 student(s).
Current registration and enrolment status: enrolled: 9/30, only registered: 0/30
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to interpret the basic principles of molecular biology and to practice the basic operations in the molecular biology used.
Learning outcomes
Fundamental knowledge of principles of methods used in molecula biology, which create basics for following lectures Drug biotechnology, Practices of drug biotechnology, Pharmacogemonics, Practices of pharmacogenomics.
Syllabus
  • 1) Isolation and characterisation of nucleic acids and proteins
    2) Centrifugation
    3) Electrophoresis - agarose, polyacrylamide, pulse-field, capillary
    4) Modifying enzymes
    5) Restriction endonucleases
    6) Polymerase chain reaction including real-time PCR
    7) Real-time PCR
    8) Southern a northern blotting, hybridisation, DNA and RNA chips
    9) Sequencing of DNA and proteins
    10) ELISA, western blotting
    11) Basic calculation in molecular biology
Literature
    required literature
  • Šmarda J, et al. Metody molekulární biologie. Masarykova Universita Brno, 2005. info
  • Sambrook a Russell. Molecular cloning, A laboratory manual. the 3rd edition. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
    recommended literature
  • Barker K. At the Bench. A laboratory navigator. Cold Spring Harbor Laboratory Press, USA, 2005. info
    not specified
  • Bustin SA. A-Z of quantitative PCR. International University Line, USA, 2004. info
Teaching methods
lectures, videos
Assessment methods
Written examination test - multiple choices and suplemented (to successfully complete 60 %)
Language of instruction
English
Further Comments
Study Materials
The course can also be completed outside the examination period.
  • Enrolment Statistics (recent)
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