F1AO2_12 Antioxidants and Free Radicals - practical classes

Faculty of Pharmacy
Autumn 2022
Extent and Intensity
0/1/0. 2 credit(s). Type of Completion: z (credit).
Teacher(s)
PharmDr. Sabína Jusková, Ph.D. (seminar tutor)
doc. PharmDr. Renata Kubínová, Ph.D. (seminar tutor)
doc. Mgr. Jan Muselík, Ph.D. (seminar tutor)
Mgr. Ing. Jiří Václavík, Ph.D. (seminar tutor)
Guaranteed by
doc. PharmDr. Renata Kubínová, Ph.D.
Department of Natural Drugs – Departments – Faculty of Pharmacy
Supplier department: Department of Natural Drugs – Departments – Faculty of Pharmacy
Timetable of Seminar Groups
F1AO2_12/01: each odd Tuesday 16:10–17:50 45-229, S. Jusková, J. Muselík, J. Václavík
F1AO2_12/02: each even Tuesday 16:10–17:50 45-229, S. Jusková, J. Muselík, J. Václavík
Prerequisites
FAKULTA(FaF) || OBOR(MUSFaF)
Fundamentals of analytical chemistry, organic chemistry, physiology and pathophysiology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 23/24, only registered: 0/24, only registered with preference (fields directly associated with the programme): 0/24
fields of study / plans the course is directly associated with
Course objectives
The aim of this subject is to offer the students with pharmacological aspects oxidative stress, chemistry of free radicals, free radical reactions, antioxidants natural origin, and methods for assesment of oxidive stress and for detection of antioxidant activity of natural compounds.
Learning outcomes
Mastering of methods for testing antioxidant activity, overview of oxidative processes in the body, oxidative stress, and its pathophysiology.
Syllabus
  • 1.Detection of reactive oxygen species
    2.Free- radicals reaction
    3.HPLC-ABTS methods for detection of antioxidant activity of compounds
    4.In vitro screening for antioxidant activity of natural compounds - spectrophotometric methods
    5.In vitro screening for antioxidant activity of natural compounds - luminiscence techniques
    6.Tyrosine nitration assay
Literature
    required literature
  • Śtípek Stanislav. Antioxidanty a volné radikály ve zdraví a v nemoci. info
Teaching methods
Practical class
Assessment methods
participation in practical class
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Passing practical exercises
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2022/F1AO2_12