FAMB1_16 Microbiology

Faculty of Pharmacy
Autumn 2023
Extent and Intensity
2/2/0. 5 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. MUDr. Josef Feit, CSc. (lecturer)
PharmDr. Jakub Treml, Ph.D. (lecturer)
PharmDr. Jakub Treml, Ph.D. (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (lecturer)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
Guaranteed by
PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Mon 8:30–10:10 45-224
  • Timetable of Seminar Groups:
FAMB1_16/01: Mon 10:15–11:55 45-323, D. Rotrekl
Prerequisites (in Czech)
FAKULTA(FaF) || OBOR(MUSFaF)
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The subject provides an overview of pharmaceutical and medical aspects of microbiology, virology, mycology and parasitology. It provides basic information on the structure, function, genetics and taxonomy of medically important bacteria, viruses, fungi, yeasts and parasites. Describes the principles of good laboratory and manufacturing practice. It deals with basic methods of prevention, prophylaxis and treatment of infectious diseases and provides information on clinically important infections in human medicine.
Learning outcomes
Basic knowledge of microbiology forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology, Pharmacogenomics.
Syllabus
  • Syllabus of lectures
    1. The importance of microorganisms in biosphere habitats. The structure of prokaryotic and eukaryotic cells. Morphology and physiology of bacteria. Bacteria reproduction. Sporulation and germination
    2. Basics of the bacteria genetics. Variability and adaptation. Expression and mutation proof. Bacterial viruses, extrachromosomal elements. Transmission of the genetic information - conjugation, transformation and transduction
    3. Structure and replication of animal viruses. Structure and proliferation of medically important fungi. Environmental impacts on microorganisms
    4. Relationships between organisms. Pathogenicity, virulence and its factors. Etiology and pathogenesis of infectious diseases
    5. Classification of bacteria, main bacterial infections
    6. Classification of antimicrobial substances, their structure and mechanism of action. Selective toxicity. Main principles of the production of antibiotics and chemotherapeutics.
    7. Resistance of microorganisms to antimicrobial substances. Principles of targeted therapy of infectious diseases.
    8. Classification of animal viruses. Main viral infections. Antivirotics.
    9. Types and therapy of infectious hepatitis
    10. Basics of medical mycology and parasitology, infections overview, therapy
    11. Prophylaxis and prevention of infectious diseases.
    12. Epidemiology principles. Nosocomial infections.
    13. Final lecture, revision.
    Syllabus of Practices
    1. Work safety in the laboratory of microbiology, main principles of Good Laboratory Practice. Equipment of the laboratory of microbiology. Cultivation boxes. Aseptic procedures.
    2. Microscopic proof of bacteria. Section preparation. Methods of staining.
    3. Disinfection and sterilization. Classification of antiseptic agents and disinfectants. Disinfection procedures in healthcare facilities
    4. Culture media, bacteria cultivation methods. Evaluation of the morphology of bacterial colonies. Determining bacteria concentrations. Isolation and identification of bacterial strains.
    5. Testing methods of bacterial sensitivity to antimicrobial substances.
    6. Methods of molecular biology in microbiology. Hybridization, PCR, DNA sequencing. Identification of resistance genes
    7. Clinical manifestations of infectious diseases, foodborne and respiratory infections
    8. Vertically transmitted diseases
    9. Late consequences of infectious diseases. Travel medicine, exotic infections
    10. Diagnostics of infectious diseases
    11. Early, skin and venereal diseases
    12. Principles of aseptic manufacturing procedures in the production of pharmaceutics. Ensuring microbiological compliance of medicinal substances and medical supplies
    13. Final classification test
Literature
    required literature
  • Votava, M. Lékařská mikrobiologie speciální. Brno, Neptun, 2003. info
  • Votava, M. Lékařská mikrobiologie obecná. Brno, Neptun, 2001. info
  • Čížek, A. Praktika z mikrobiologie pro farmaceuty. Brno, ÚMI FVL VFU Brno, 2001. info
    recommended literature
  • Buchta, V. Základy mikrobiologie a parazitologie pro farmaceuty. Karolinum, 1998. info
    not specified
  • Bednář, M. Lékařská mikrobiologie. Praha, Marvil, 1999. info
  • Hugo, W. B., Russel, A. D. Pharmaceutical microbiology. Blackwell Science, 1998. info
Teaching methods
Lectures and theoretical seminars that prepare for laboratory exercises. The output of the exercise are independently processed protocols.
Assessment methods
The course is completed by a short written test and oral exam.
Language of instruction
English
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
Teacher's information
To obtain the credit, students must fulfill the obligatory 100 % attendance of practices. To pass the exam, students must successfully write the classification test (min. 60 %) on the regular date. The correction tests shall have a form of oral exam.
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2022, Autumn 2024.
  • Enrolment Statistics (Autumn 2023, recent)
  • Permalink: https://is.muni.cz/course/pharm/autumn2023/FAMB1_16