F1FY1_12 Phytochemistry

Faculty of Pharmacy
Spring 2020
Extent and Intensity
1/1/0. 3 credit(s). Type of Completion: z (credit).
Teacher(s)
prof. PharmDr. Karel Šmejkal, Ph.D. (lecturer)
PharmDr. Marie Čulenová, Ph.D. (seminar tutor)
PharmDr. Markéta Gazdová, Ph.D. (seminar tutor)
PharmDr. Dagmar Jankovská, Ph.D. (seminar tutor)
PharmDr. Alice Sychrová, Ph.D. (seminar tutor)
prof. PharmDr. Karel Šmejkal, Ph.D. (seminar tutor)
Guaranteed by
prof. PharmDr. Karel Šmejkal, Ph.D.
Department of Natural Drugs – Departments – Faculty of Pharmacy
Prerequisites
The students should have passed basic subject touching organic chemistry, analytical chemistry, biology, botany, and physiology.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The brief anotation determinating targets of the study subject and the content direction:
The targets of the study subject: The subject investigates the relationships of the primary and secondary metabolism of the plants and is directed on the study of the secondary metabolites, their biosynthesis, their properties, chemical structure and the ways of their isolation from the plant material. In the scope of this subject students get acquainted with important pharmaceutical plants, with their collection and conversion to drugs.
Learning outcomes
Basic knowledge of secondary metabolites and methods for their analysis.
Syllabus
  • 1 Phytochemistry as the field of science, primary and secondary metabolism.
    2 Principles of separation and isolation of natural substances.
    3 Systems for classification of natural compounds, secondary metabolites.
    4 Chromatography I.
    5 Chromatography II.
    6 Identification of Natural Products
    7 High-performance liquid chromatography, gas chromatography
    8 Spectral methods for identifying natural compounds (UV, IR).
    9 Spectral methods for identifying natural compounds (MS).
    Laboratory of phytochemistry
    1 Introduction, safety, theoretical basics of methods for obtaining content substances from plants
    2 Isolation of Natural Products - Floral pigment of Urticae herba extraction (Soxhlet), caffeine microsublimation, Species urologicae compounds extraction (maceration), hydrodistillation of essential oils
    3 Separation of natural substances - Thin layer chromatography of floral pigments of Urticae herba extract and phenolic glycosides from the extract of Species urologicae
    4 Separation of Natural Products II - Column chromatography of floral pigments
    5 Separation and identification of natural substances
    Determination of carotenoid pigments by spectrophotometry, measuring of the melting point of crystalline natural substances, SFE demonstration, IR spectrometry demonstration.
    6 Identification of Natural Products
    High-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), HPLC / MS,
    7 Final test
Literature
    recommended literature
  • Šmejkal K, Muselík J, Mokrý P. Laboratorní metody experimentální fytochemie. Brno, 2013. ISBN 978-80-7305-649-0. info
  • Walton NJ, Brown DJ. Chemicals from Plants: Perspectives on Plant Secondary Products. 1999. ISBN 981-02-2773-6. info
  • Opletal L.,Drašar P. Fytochemické metody: 1. izolace obsahových látek. UK Praha Karolinum, 1994. info
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course can also be completed outside the examination period.
Teacher's information
100% attendance of the practical classes
minimum of 60 % of the classification tests
Protocols of exercises
The course is also listed under the following terms Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2020, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2020/F1FY1_12