FaF:F1PB1_16 Pathobiochemistry - Course Information

F1PB1_16 Pathobiochemistry

Extent and Intensity

2/1/0. 4 credit(s). Type of Completion: zk (examination).

Teacher(s)

Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Marek Petr, Ph.D. (lecturer)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
Mgr. Robert Helma, Ph.D. (seminar tutor)
Mgr. Marek Petr, Ph.D. (seminar tutor)
PharmDr. Zuzana Soldánová (seminar tutor)

Guaranteed by

Mgr. Marie Brázdová, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• Pharmacy (programme FaF, M-FARM)

Course objectives

Pathobiochemistry follows the course of Biochemistry taught in the winter semester. Biochemistry deals with the metabolic processes in living organisms.
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy.
The aim of the practical exercises are analyzes of some selected diagnostically important compounds, the correct interpretation of the results of analyzes and their use for the purpose of human diagnostic and therapeutic practice.

Syllabus

• Syllabus Pathobiochemistry
  
  1. Introduction, the importance of studying pathobiochemistry. The scope and requirements for successful completion of the course exam, recommended literature. Understanding the regulation of metabolism. Hormones. Biochemical communication.
  2. Metabolism disorders, types and causes. Hereditary metabolic diseases. Enzymes, regulation of metabolism. Causes increased activity of cellular enzymes in the plasma. Clinically significant enzymes.
  3. Pathobiochemistry of carbohydrates, glucose metabolism and its disorders. Glycemic control disorders. Pathobiochemistry of diabetes mellitus, types of DM. Disorders of glycogen metabolism, glykogenosis.
  4. Amino acid metabolism and its disorders. Types of diseases and therapy.
  5. Disorders of lipid metabolism. Cholesterol, lipoproteins. Lipidosy, dyslipoproteinaemia.
  6. Pathobiochemistry of arteriosclerosis. Ischemic heart failure - cardiac markers.
  7. The nucleic acid metabolism disorders of purine and pyrimidine. Hyperuricemia, orotacidurie, therapy.
  8. Blood, blood plasma proteins. Blood clotting, coagulopathy. Dysproteinaemias. Porphyrins. Biosynthesis, metabolism disorders. Porphyria, hemoglobinopathies.
  9. Xenobiotics and their effects on the body. Detoxification mechanism. Biological oxidation. The effects of free radicals on the organism. Lipoperoxidation antioxidants.
  10. Tumor, tumor markers. Basic characteristics of tumor cells. Strategy laboratory tests. Requirements ideal tumor marker. Used tumor markers.
  11. Relation between Pathobiochemistry and Clinical Biochemistry. Clinical and biochemical analysis and its specific features. Terminology of Clinical Biochemistry. The analyzed material. Material removal.
  12. Analysis of urea and the urinary sediment. Immunochemical methods.
  13. Mechanization and automation in clinical biochemistry. Analyzers, their distribution from different perspectives. Diagnostic kits. The organization of work in clinical-biochemical laboratory, laboratory and hospital information systems.
  Sylabus of practical exercises in pathobiochemistry:
  1. Exercise: Analysis of tumor suppressor by immunodetection on the membrane.
  2. Exercise: Basic biochemical determinations using a biochemical analyzer Dimension.
  3. Exercise: Immunochemical methods. Analyzer Immulite Immunoassay Analyzer.
  4. Exercise: Hematological methods. Use of automated hematology analyzers in clinical practice. Coagulation methods.
  5. Exercise: Final test
  Guarantors: Mgr. Marie Brázdová, Ph.D.,
  Tutorial lecturers:
  Mgr. Marie BRÁZDOVÁ, Ph.D.
  Mgr. J. Jelinek, Mgr. I. Kučerová, Mgr. Vesely, Mgr. Čech
  Mgr. A. Polášková, Mgr. R. Helm, Mgr. M. Peter, Mgr. Adamík
  Lecturers:
  Mgr. Marie BRÁZDOVÁ, Ph.D.
  Mgr. J. Jelinek, Mgr. I. Kučerová, Mgr. Vesely, Mgr. Čech
  Garant of discipline:
  Mgr. Marie Brázdová, PhD.
  
  

Literature

required literature
• G.F,.Hoffmann et al. Dědičné metabolické poruchy. Grada,, 2012. info

recommended literature
• J.Koolman, K.H.Rohm. Barevný atlas biochemie. Grada, 2012. info
• Murray et al. Harpeŕ´s Illustrated Biochemistry. 29th Edition. Lange, 2012. info
• Robbins SL, Cotrans RS, Kumar V. The Pathologic Basis of Disease, 3rd Edition. Saunders, 2010. info
• Ledvina a kol. Biochemie pro studující medicíny - I. a II.díl. Karolinum, 2005. info
• KARLSON, P., GEROK, W., GROSS, W. Pathobiochemie. Academia, Praha, 1987. info

Language of instruction

Czech

Further comments (probably available only in Czech)

The course can also be completed outside the examination period.

• Enrolment Statistics (Spring 2020, recent)
  
• Permalink: https://is.muni.cz/course/pharm/spring2020/F1PB1_16