FAAI2_15 Analytical Chemistry II

Faculty of Pharmacy
Spring 2021
Extent and Intensity
2/3/0. 7 credit(s). Type of Completion: zk (examination).
Teacher(s)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (lecturer)
Ing. Klára Odehnalová, Ph.D. (seminar tutor)
doc. RNDr. Bc. Jiří Pazourek, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Bc. Jiří Pazourek, Ph.D.
Department of Chemical Drugs – Departments – Faculty of Pharmacy
Timetable
Wed 13:00–14:30 44-037
  • Timetable of Seminar Groups:
FAAI2_15/01: each even Thursday 8:00–12:30 44-339, J. Pazourek
Prerequisites
getting grades for 3150/F1AI1
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 24 student(s).
Current registration and enrolment status: enrolled: 1/24, only registered: 0/24
fields of study / plans the course is directly associated with
Course objectives
Analytical chemistry
The aim is acquiring theoretical and practical knowledge for qualified
chemical and instrumental analysis of inorganic and organic materials.

Practical training is aimed at acquiring good laboratory practice and
creative approach to solving analytical problems.
Learning outcomes
After completing the course the student will be able to: - understand the meaning and principles of classical and modern quantitative analytical chemistry - have an overview of methods and practically perform gravimetry, volumetry, photometry, potentiometric titration, HPLC and capillary electrophoresis
Syllabus
  • Lessons:
    1.-2. Quantitative analysis: basic concepts and methods of quantitative analysis. Sample preparation, dissolution of samples. Solutions, expressing their composition, the measurement of volume. Gravimetry, laboratory balances, calculations in gravimetry. Expression of results (the gravimetric factor).
    3.-6. Introduction to volumetric analysis: definition. Standard solutions, primary standards, finding the exact concentration of standard solutions (standardisation). Types of titrations, titration curve, indications of the end point. Alkalimetry, acidimetry, complexometry, argentometry, redox titrations. Use volumetric methods in pharmaceutical analysis.
    7.-8. The basics of optical methods. Properties of the electromagnetic radiation: interaction of mass and radiation. The emission, absorption of radiation, change of the direction and speed, optical rotation, magnetic field effect. Principles and utilization of optical methods in pharmaceutical analysis.
    9.-10. Basics of electroanalytical methods: basic concepts and principles - electrode, galvanic cell, electrolytic cell. The methods used in pharmaceutical analysis: potentiometry, voltammetry, polarography, striping voltammetry.
    11.-12. Introduction to separation methods: Classification, basic principles, examples of applications. Extraction, SPE. Chromatographic methods (IEC, GC, HPLC, TLC).
    13.-14. Electromigration methods (CZE, IEF, ITP, CE). Detectors. Typical applications
    Laboratory exercises (manuals in MOODLE) :
    1. Gravimetric Determination of Iron in the Form of Ferric Oxide
    2. Volumetric Determination of an Insoluble Carbonate by Back Titration
    3. Complexometric Determination of Bismuth and Zinc by Stepwise Titration
    4. Spectrophotometric determination of copper with Complexon III
    5. Argentometric determination of halides mixture with potentiometric end-point indication
    6. Determination of caffeine in tablets of Acifein by high-performance liquid chromatography (HPLC)
    7. Determination of acetylsalicylic acid in tablets of Acifein by capillary zone electrophoresis (CZE)

Literature
    required literature
  • Skoog, West. Fundamentals of Analytical Chemistry. URL URL info
    recommended literature
  • European Pharmacopoeia. URL URL info
Teaching methods
oral lessons laboratory exercises
Assessment methods
written test (online)
Language of instruction
English
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
To gain credits, a student must:
*take part in all the laboratory exercises
*hand over reports from all the labs
*pass a final examination test with a minimum of 51%
Requirements for examination:
Analytical reactions: types of analytical reactions, requirements for qualitative (proof) and quantitative (determination) analysis. Agent, analyte, sample, matrix.
Protolytic reactions: water as a solvent, definition of pH, calculation of pH of aqueous solutions, weak/strong acids and bases, buffers.
Qualitative chemical analysis: general procedure of qualitative analysis.
Tests for important inorganic cations and anions.
Proof and identification of organic compounds: preliminary tests. Determination of basic physical properties (constants). Elemental analysis. Solubility classes. Tests for oxygen and nitrogen functional groups. Classical and modern methods of identification of organic compounds.
Quantitative analysis: basic terms, method classification. General procedure of quantitative analysis. Calculations and results evaluation. Sample preparation, dissolution of samples. Solutions, expression of solution concentrations, precise volume measurement.
Gravimetry, analytical balances and weighing, calculations in gravimetry, Gravimetry factor.
Volumetry: volumetric solutions, primary standards, determination of precise concentration of volumetric solutions. Indication of the titration end-point - indicators. Types of titrations, titration curves. Precipitation titrations - the solubility product. Complexometric titrations - stability constants. Principles of volumetric methods - alkalimetry, acidimetry, complexometry, argentometry, redox titrations. Calculations in volumetric analysis - end-point determination.
Instrumental methods - determination with calibration curve.
Optical methods: interaction of mass and radiation, electromagnetic radiation (EMG). Classification of optical methods (radiation absorption/emission, changes of direction, speed and optical rotation of radiation). Principles instrumentation and applications of optical methods in pharmaceutical analysis - molecular and atomic methods, absorption and emisson analysis (UV-VIS, infrared spectroscopy, Raman spectroscopy, AAS, AES, ICP-MS), refractometry, polarimetry, circular dichroism (CD). Nephelometry, turbidimetry. Absorbance, blank sample, spectrum.
Electroanalytical methods: basic terms and principles, redox analytical reactions, redox potential, its measurements, factors affecting the redox potential, electrochemical series of reduction potentials.
Electrochemical methods used in pharmaceutical analysis - potentiometry (type of electrodes incl. ISE), potentiometric titrations, Voltametry - polarography, coulometry, electrogravimetry, conductometry.
Separation methods: classification, basic principles. Extraction - LLC. Chromatographic methods (TLC, HPLC, IEC, GC). Special detectors in chromatographic methods (FID, ECD, MS).
Electromigration methods (CZE, IEF, ITP, HPCE). Detection in electromigration methods. Evaluation of chromatograms - capacity factor k´, efficiency of a column N, HETP, resolution Rs. Evaluation of electroforeogram - electroosmotic flow (EOF), electrophoretic mobility u.
The course is also listed under the following terms Spring 2020, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2021, recent)
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