FaF:FABL2_16 Biotechnology of Drugs - Pract - Course Information
FABL2_16 Biotechnology of Drugs - Practical Classes
Faculty of PharmacySpring 2025
- Extent and Intensity
- 0/2/0. 2 credit(s). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- PharmDr. Dominik Rotrekl, Ph.D. (seminar tutor)
PharmDr. Jakub Treml, Ph.D. (seminar tutor) - Guaranteed by
- PharmDr. Jakub Treml, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy - Prerequisites
- NOW( FABL1_16 Biotechnology of Drugs )
The knowledge of subject in range of lectures.s - Course Enrolment Limitations
- The course is only offered to the students of the study fields the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12 - fields of study / plans the course is directly associated with
- Multidisciplinary studies at Faculty of Pharmacy (programme CST, KOS)
- Pharmacy (programme FaF, M-FARMA)
- Course objectives
- This subject is mainly focused on cytology, molecular biology and microbiology principles, which are used in recombinant therapeutics preparation.
Students will be familiarized with recombinant techniques, gene and protein engineering, and gene therapy as well.
Attention will be also paid to industrial biotechnology and production of significant products, such as antibiotics, vitamins, organic acids, amino-acids and alkaloids. - Learning outcomes
- Basic knowledge in methods of pharmaceutical biotechnology
- Syllabus
- 1) Isolation of plant chromosomal DNA
2) Amplification of selected plant gene by polymerase chain reaction
3) Purification of PCR products
4) Isolation of plasmid DNA
5) Nucleic acids analysis by agarose gel electrophoresis, spectrophotometry
6) Digestion of vector DNA by restriction endonucleases
7) Ligation of PCR product into a vector
8) Transformation of bacterial cells Escherichia coli
9) PAGE
- 1) Isolation of plant chromosomal DNA
- Literature
- required literature
- Bartoš, M,, Forejtníková, H., Bartošová, L. Biotechnologie pro farmaceuty (Návody k praktickým cvičením). VFU Brno, 2006. info
- Šmarda, J, et al. Metody molekulární biologie. Masarykova univerzita v Brně, 2005. info
- not specified
- Sambrook, J., Russell, D. W. Molecular cloning, A laboratory manual. Cold Spring Harbor Laboratory Press, New York, U, 2001. info
- Teaching methods
- LAabs
- Assessment methods
- Analysis of student work products (technical work)
- Language of instruction
- English
- Further Comments
- The course can also be completed outside the examination period.
- Enrolment Statistics (Spring 2025, recent)
- Permalink: https://is.muni.cz/course/pharm/spring2025/FABL2_16