PřF:Bi6589 Plant biosystematics methods - Course Information
Bi6589 Laboratory and bioinformatic methods of plant biosystematics
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 0/3/0. 3 credit(s) (plus extra credits for completion). Type of Completion: k (colloquium).
In-person direct teaching - Teacher(s)
- prof. RNDr. Petr Bureš, Ph.D. (seminar tutor)
Mgr. Petra Šarhanová, Ph.D. (seminar tutor)
Ing. Jakub Šmerda, Ph.D. (seminar tutor) - Guaranteed by
- prof. RNDr. Petr Bureš, Ph.D.
Department of Botany and Zoology – Biology Section – Faculty of Science
Contact Person: Ing. Jakub Šmerda, Ph.D.
Supplier department: Department of Botany and Zoology – Biology Section – Faculty of Science - Timetable
- Wed 9:00–11:50 D31/239
- Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Plant Biosystematics (programme PřF, N-BOT)
- Ecological and Evolutionary Biology (programme PřF, B-EKB)
- Course objectives
- Theoretical and practical introduction to basic laboratory, molecular biological and bioinformatic methods of plant biosystematics.
- Learning outcomes
- The student will learn to solve questions of biosystematics and taxonomy of plants using different methodological approaches.
The student will acquire theoretical knowledge about the strategy of collecting and holding plant material, basic laboratory, molecular biological and bioinformatics methods of plant biosystematics.
Students will acquire basic practice in the molecular biological, cytometric and karyological laboratories at the same time should better understand the results and arguments presented in biological journals. - Syllabus
- The concept of the subject is based on a model situation where there are two species from the same genus and individuals with intermediate characters between the species of interest. Using different methodological approaches, students will test whether difficult-to-classify individuals represent a manifestation of phenotypic plasticity of one/both species or whether it is a consequence of interspecies hybridization.
- From the methodological approaches, will be presented: strategies for collecting and holding of plant material, basics of morphometrics, flow cytometry, karyological methods; students will also be introduced to the theoretical and practical basics of molecular biology (isolation and quantification of DNA, PCR, electrophoresis, sequencing). The course will also include an introduction to and work with selected statistical and bioinformatics software (R, MEGA, STRUCTURE, SplitsTree, etc.), which will enable analysis and visualization of the obtained data.
- The all knowledge should provide enough evidence to support one of the hypotheses.
- Students will prepare a summary paper in which they will present methodological approaches, partial analyzes and a final evaluation. Credit will be awarded based on this paper.
- Literature
- required literature
- MICHÁLKOVÁ, Ester, Jakub ŠMERDA, Klára PLAČKOVÁ, Aleš KNOLL and Petr BUREŠ. Hybridization may endanger the rare North Apennine endemic Cirsium bertolonii. Plant Systematics and Evolution. Springer, 2023, vol. 309, No 3, p. 1-21. ISSN 0378-2697. Available from: https://dx.doi.org/10.1007/s00606-023-01854-2. URL info
- BUREŠ, Petr, Jakub ŠMERDA, Ester MICHÁLKOVÁ, Petr ŠMARDA, Aleš KNOLL and Martin VAVRINEC. Cirsium greimleri: a new species of thistle endemic to the Eastern Alps and Dinarides. Preslia. Česká botanická společnost, 2018, vol. 90, No 2, p. 105-134. ISSN 0032-7786. Available from: https://dx.doi.org/10.23855/preslia.2018.105. URL info
- MICHÁLKOVÁ, Ester, Jakub ŠMERDA, Aleš KNOLL and Petr BUREŠ. Cirsium ×sudae: a new interspecific hybrid between rare Alpine thistles. Preslia. Česká botanická společnost, 2018, vol. 90, No 4, p. 347-365. ISSN 0032-7786. Available from: https://dx.doi.org/10.23855/preslia.2018.347. URL info
- recommended literature
- HEYWOOD, V. H. Modern methods in plant taxonomy. 1st ed. London & New York: Academic Press, 1968, 312 pp. ISBN 68-9103. info
- BUREŠ, Petr. Cirsium Mill. – pcháč (Cirsium Mill. – thistle). In Zdeněk Kaplan. Klíč ke květeně České republiky. Druhé, přepracované. Praha: Academia, 2019, p. 1034-1036. 11961. ISBN 978-80-200-2660-6. info
- ŠMARDA, Jan, Jiří DOŠKAŘ, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ and Jana KOPTÍKOVÁ. Metody molekulární biologie (Methods of molecular biology). 2. dotisk 1. vydání. Brno: Masarykova univerzita, 2010, 194 pp. ISBN 978-80-210-3841-7. info
- BUREŠ, Petr. Cirsium Mill. - pcháč. In Klíč ke květeně České republiky. 1st ed. Praha: Academia, 2002, p. 669-671. 4928. ISBN 80-200-0836-5. info
- SILVERTOWN, Jonathan and Deborah CHARLESWORTH. Introduction to plant population biology. 4th ed. Oxford: Blackwell Science, 2001, viii, 347. ISBN 0-632-04991-X. info
- Teaching methods
- Teaching takes the form of theoretical lectures and practical exercises. Practical methods are explored by students in the laboratory and by working with selected software. Students will regularly prepare homeworks, the fulfillment of which is a prerequisite for entering the next lesson, as homework often involves preparing the data needed for the next lesson.
- Assessment methods
- To get the credit for the course, students have to elaborate final report, which includes a brief introduction to the working hypotheses, description of methodological approaches, analysis of the obtained data and subsequent interpretation of the results. To pass the course attendance is required in classes. Two absences are possible. However, in the case absences, the obligation to prepare a homework for the next lesson remains.
- Language of instruction
- Czech
- Further Comments
- The course is taught annually.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/Bi6589