Bi7230 Advanced methods of biophysics in experimental biology

Faculty of Science
Autumn 2012
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Ctirad Hofr, Ph.D. (lecturer)
Guaranteed by
prof. RNDr. Jiří Fajkus, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Ctirad Hofr, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 9:00–10:50 C02/211
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 14 fields of study the course is directly associated with, display
Course objectives
The lecture course is focused on acquiring theoretical knowledge needed for implementation of modern spectroscopic methods for analysis of protein-protein and protein-DNA interactions. From the methodical approaches, the main emphasis is put on highly sensitive fluorescent methods and their practical use in biological laboratories. The main lecture topics are detection, techniques for fluorescent labeling of macromolecules and their application for analysis of function and interaction of important biomolecules in genome and proteome.
Syllabus
  • Definition and characteristics of absorption, fluorescence and phosphorescence
  • Instrumentation - spectrophotometer, spectrofluorometer
  • Steady-state fluorescence
  • Time-resolved fluorescence
  • Fluorescence anisotropy and its change after interaction of macromolecules
  • Absorption spectroscopy in biological analysis of molecules
  • Fluorescence quenching, resonance energy transfer - application for studies of structural changes of biomolecules
  • Intrinsic fluorescence of biologically important molecules
  • Extrinsic fluorescence – fluorescent labels, probes, and indicators
  • Fluorescence labeling of DNA and proteins
  • Fluorescence microscopy
  • Analytical applications of fluorescence for concentration determination
  • Examples of fluorescence application in biology
Literature
  • Lakowicz J.R.: Principles of Fluorescence Spectroscopy. Third Edition, Springer + Business Media, New York, 2006.
Assessment methods
lectures, written test + oral exam
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Teacher's information
http://is.muni.cz/el/1431/podzim2011/Bi7230/um/6053971/PBMEBuvod.html
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation.
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