PřF:C3620 Biochemistry-lab.course - Course Information
C3620 Biochemistry - laboratory course
Faculty of ScienceAutumn 2024
- Extent and Intensity
- 0/3/0. 3 credit(s). Type of Completion: z (credit).
In-person direct teaching - Teacher(s)
- Mgr. Lukáš Faltinek (seminar tutor)
Mgr. Marta Pelcová, Ph.D. (seminar tutor)
Mgr. Vojtěch Sedláček, Ph.D. (seminar tutor) - Guaranteed by
- doc. Mgr. Jan Lochman, Ph.D.
Department of Biochemistry – Chemistry Section – Faculty of Science
Contact Person: doc. Mgr. Jan Lochman, Ph.D.
Supplier department: Department of Biochemistry – Chemistry Section – Faculty of Science - Timetable of Seminar Groups
- C3620/01: Wed 16:00–18:50 C15/212, V. Sedláček
C3620/02: Mon 14:00–16:50 C15/212, M. Pelcová - Prerequisites (in Czech)
- NOW( C3580 Biochemistry )
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- At the end of the course students should be able to understand and explain - basic properties of natural substances (aminoacids, carbohydrates, lipids, nucleic acids, proteins, enzymes and coenzymes, their properties and structure, active site), thermodynamics, enzyme kinetics, inhibition, allostery, regulation of enzyme activity.
- Learning outcomes
- Student will be able to: - Perform basic laboratory operations in the biochemical laboratory - Control common laboratory instruments used in the biochemical laboratory - Mathematically evaluate measured data - Interpret the simple results of his work
- Syllabus
- 01. Work safety rules. Organization of laboratory exercises. Preparation of dilute solution, pipetting, and photometry.
- 02. Isolation, analysis, and determination of deoxyribonucleic acid (DNA).
- 03. Qualitative and quantitative determination of amino acids.
- 04. Quantitative determination of proteins.
- 05. Qualitative and quantitative determination of carbohydrates.
- 06. Lipids and their analysis.
- 07. Sucrase - immobilization, substrate specificity, activity determination.
- 08. pH optimum of enzyme reaction.
- 09. Enzyme kinetics.
- 10. Analytical use of enzymes.
- 11. Biological electron transfer chains.
- Literature
- Teaching methods
- laboratory courses
- Assessment methods
- The first exercise is devoted to learning about safety in biochemical laboratory. Students also write the entrance test, whose successful completion is a prerequisite for participation in the exercise. Before each next exercise is a written test verifies the student's preparation for the job. In all tests, including entrance test, the student must correctly answer at least 60% of the questions.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- Study Materials
The course is taught annually.
General note: Vyučují učitelé katedry biochemie. - Listed among pre-requisites of other courses
- C6260 Methods of Protein Separation
!C6200 && ( C3580 && (C3600 || C3620))
- C6260 Methods of Protein Separation
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/autumn2024/C3620